1,720,995 research outputs found
From Cells to QSAR: Alternative predictive models in toxicology
No full textmeeting report on From Cells to QSAR: Alternative predictive models in toxicology, hosted by The Italian Association for In Vitro Toxicology CELLTOX in collaboration with the Laboratory of Analysis and research in Physiopathology (LARF), Department of Experimental Medicine (DIMES) of the University of Genova, in April 2016. The meeeting was a a three-day course on alternative predictive models in toxicology. The course was also supported by ESTIV, The European Society of Toxicology In Vitro. Among speakers there were many international relevant experts in Toxicology in vitro, such as Thomas Hartung, Professor of Evidence Based Toxicology and Director of the Center for Alternatives to Animal Testing (CAAT) at the Johns Hopkins University in Baltimore (US) . Laura Gribaldo, from JRC (Ispra, Italy); Chantra Eskes (SECAM, Switzerland); Silvia Letasiova from MatTek (Bratislava, Slovakia); and other experts.The practical session included two training blocks, focused on a human skin model for prediction of the irritation potential of chemicals, and on 3D dynamic in vitro models-i A panel discussion among experts and trainees, during hands-on sessions and in the final roundtable, contributed to create a scientific network on alternative methods
Assessment of neurotoxicity assay based on neural-like cells from human adipose derived stem cells
No full text. In vitro animal models of the nervous system have been in use for many years provide an important tool for functional studies at cellular and molecular levels.
Aim of our study was to generate standardized neural precursor populations from human Adipose-derived Stem Cells (hADSC), by tissue, routinely discarded after liposuction surgeries. hADSC were submitted to neural differentiated procedures, and positiveness for Nestinn and GFAP confirmed their commitment to neural like cells (N-hADSC).
To test the suitability of N-hADSC as model for neural toxicity assessment, N-hADSC and rat cortical neuron cells were exposed for 24-48 hours to three neurotoxic compounds ( Carbaryl, Lidocaine and Phenytoin). A different behavior confirm the need of specie-specificity in toxicity tests
Development of in vitro testing strategies for toxicity evaluation of mixtures derived from discarded laboratory chemicals
No full textOur complementary methods can represent an high content sensitive tool to assess the safety of chemical mixtures, that can act on skin, as well on eye and neural functions.
Aim of this study was to assess the toxic potential of two mixtures (Cat. EWC160506, one inorganic and one organic), by different in vitro approaches
Effects of in vitro exposure to lipoperoxides in human colon adenocarcinoma and normal endotheliocyte cells are dependent by ROS-modulation of angiogenesis and proliferation markers.
No full textOxidation antitumor therapy is developed by delivering excess oxidative stress into tumor cells or targetedly disrupting the antioxidant systems. In present study, several markers related to oxidative stress, proliferation and angiogenesis were evaluated in human DLD-1 colon carcinoma, and HECV normal endothelial cells, during exposure to 0.1-1% Lipopeorxides (LOOH). To mimic the environment surrounding neoplasm mass, HECV are also exposed to conditioned medium derived by DLD-1 subjected or not to LOOHs. Observed LOOH cytostatic effects on tumor cells and angiogenesis inhibition, may be related to an imbalance of redox status and an increase of oxidative stress.
Thus LOOHs may be promising in co-adjuvant tumor therap
Extramitochondrial oxidative phosphorylation in myelin sheath: reactive oxygen species production and axonal degeneration in demyelinating diseases.
No full textA comparative study of leukaemia inhibitory factor and interleukin-1? intracellular content in a human keratinocyte cell line after exposure to cosmetic fragrances and sodium dodecyl sulphate
No full textAccording to European laws animal testing in cosmetic industry will be prohibited in a few years and it will be replaced by alternative methods based on cell and tissue culture. Many ingredients of cosmetic formulations are potentially causes of skin inflammation and sensibilization. Since cytotoxicity is known, among other factors, to trigger irritation, in an alternative model for evaluation of skin irritation, it can be considered also the precocious release of inflammatory mediators, i.e. cytokines, originating mainly
from keratinocytes.
In this in vitro study we have analysed some parameters directly or indirectly related to irritation/inflammation, in NCTC 2544 human keratinocytes during short-time exposure to some potential
irritants cosmetic fragrances, included in the European Laws 2003/15/EEC. IIC50 was extrapolated byMTT and NRU viability indexes after exposure of cell ultures to Geraniol Limonene and Benzylic Alcohol for 1, 3 and 6 h. NCTC cells were then exposed to sub-toxic doses of selected compounds and interleukin-1 (IL-1) and leukaemia inhibitory factor (LIF) expressions were analysed as early proinflammatory cytokines.
To our knowledge our findings demonstrated for the first time that NCTC cells synthesize and modulate LIF after exposure to selected irritating stimuli. Moreover, our results give evidence on LIF role as in vitro precocious endpoint for the assessment of the risk in cosmetic field, because its response under irritation stimuli is very quick and comparable to IL-1
Effetti di Aloe arborescens su proliferazione e equilibrio ossidativo in cellule di carcinoma umano del colon.
No full textExtramitochondrial oxidative phosphorylation in myelin sheath: reactive oxygen species production and axonal degeneration in demyelinating diseases.
No full textEffects of Aloe arborescens whole-leaf juice on proliferation rate and oxidative status in human colon carcinoma and normal endothelial cells.
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