102,038 research outputs found
EEF1A1 (eukaryotic translation elongation factor 1 alpha 1). Atlas Genet Cytogenet Oncol Haematol. May 2009 .
No full textAtlas of Genetics and Cytogenetics in Oncology and Haematolog
Interaction of G-rich GT oligonucleotides with nuclear-associated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines.
No full textG-rich GT oligonucleotides with a different content of G clusters have beenevaluated for their ability to exert cytotoxicity and to bind to nuclear-associatedproteins in T-lymphoblast CCRF-CEM cells. Only the oligomersthat did not form G-based structures or had a poor structure, under physiologicalconditions, were able to exert significant cellular growth inhibitioneffect. The cytotoxicity of these oligomers was related to their bindingto the nuclear-associated eEF1A protein, but not to the recognition ofnucleolin or other proteins. In particular, GT oligomers adopting a conformationcompatible with G-quadruplex, did not exert cytotoxicity and didnot bind to eEF1A. The overall results suggest that the ability of oligomersto adopt a G-quadruplex-type secondary structure in a physiological buffercontaining 150 mm NaCl is not a prerequisite for antiproliferative effect inhaematopoietic cancer cells. The cytotoxicity of G-rich GT oligomers wasshown to be tightly related to their binding affinity for eEF1A protein
Aptameric GT oligomers need to be complexed to ethoxylated polyethylenimine as pre-paired diplex to efficently exert their cytotoxic activity in human lynphoblastic cancer cells.
No full textThe aptameric oligonucleotides GT were found to exert a selective, specific and dose-dependent cell growth inhibition effect on a varietyof human cancer cells by recognising specific nuclear proteins and among these in particular an isoform of the eukaryotic elongation factor1A1 (EEF1A1). The potential development of these aptameric oligomers needs that they retain serum and intracellular stabilities. Polycationsare safe non-viral carriers of the nucleic acids.We demonstrated that a weakly basic polycation, the ethoxylated polyethylenimine (EPEI), canefficiently deliver cytotoxic GT oligomers when they were complexed as partial pre-paired duplex. In this way, nuclease-resistance of theoligomer was markedly improved and the administration of the duplex complexed with EPEI to lymphoblastic cancer cells caused a specificcytotoxic effect at concentrations lower than that of naked GT. However, the cytotoxic activity of the oligomer-EPEI complex resulted strictlyrelated to the GC content and Tm of the duplex region. The single-stranded GT and the duplex with high GC content and Tm, althoughcomplexed with EPEI failed to exert cytotoxicity. Overall results indicated that aptameric oligomers complexed with polycations can beefficiently delivered into the cells and display the desired biological effect designing a balanced partial duplex whose stability can allowoligomer release from the polycation under the physiological cellular conditions
A Single-Center Experience With Phoenix Atherectomy System in Patients With Moderate to Heavily Calcified Femoropopliteal Lesions
No full textPurpose: To evaluate efficacy and safety of a new rotational atherectomy (RA), the Phoenix AtherectomyTM System, for the treatment of de novo and re-stenotic or occlusions atherosclerotic moderate-heavily lesions of the femoro-popliteal axis. Material and methods: From January 2015 to August 2017, 52 patients with heavily calcified femoro-popliteal lesions causing severe stenosis or occlusions were enrolled in our center to be treated using Phoenix catheters. Primary endpoints of this study were acute efficacy and safety at 30 days. Secondary endpoints were freedom from restenosis and target lesion revascularization (TLR)/target vessel revascularization (TVR) at 1-, 6- and 12- months. Results: The mean lesion length was 9.2 cm (range 5-23 cm). The lesions were located in superficial femoral artery (SFA) in 61.5% (Fig. 1-A), in popliteal artery in 21.1% and involved femoral-popliteal axis in 15.4%. A primary technical success was achieved in 51/52 patients, with an optimal working channel after RA alone. Using Kaplan-Meyer analysis, primary vessel patency rates at 1, 6 and 12 -months was 96.1%, 86.5% and 76.9% respectively. Assisted primary patency at 1, 6- and 12 -months was 100%, 90.3% and 86.5% respectively. Conclusions: Recanalisation with the Phoenix Atherectomy System is simple and safe, with a high technical success rate
Winegrape Production Costs: A Comparison Of Different Training Systems Widely Used In Italy
No full text
Multifunctionality in Producers of Prosecco D.O.C. di Conegliano e Valdobbiadene: Analysis of some case studies and repercussions on the territory
No full textTriple-helix forming oligodeoxyribonucleotide, inhibiting mdr1 transcription may reduce mdr1 gene amplification level in multidrug-resistant leukaemic cell line
No full text- …
