102 research outputs found
Lecba diabetu transplantaci inzulin produkujici tkane
No full textThe optimal goal of pancreas or islet of Langerhans transplantation is to normalize the intermediary metabolism of patients with Type-1 diabetes mellitus thereby preventing the development of late organ complications. To date, only whole pancreas transplantation has been clinically successful. Studies monitoring the course of advanced diabetic nephropathy demonstrated that development of chronic renal insufficiency in patients with Type-1 diabetes is very rapid and patients prognosis grim. Combined kidney and pancreas transplantation may dramatically improve the survival rates and quality of life. We found that, compared with segmental transplantation with pancreatic duct draining into the bladder is associated not only with longer graft survival rates but, also, with a higher degree of glucose tolerance. The achieved results have been instrumental in establishing pancreas transplantation as the most suitable therapeutic method for selected recipients and have created the preconditions for future study of preparing human islets of Langerhans for transplantation in manAvailable from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi
Changes in Tyden magazine after Frantisek Nachtigalls editor in chief
No full textThe bachelor's thesis Changes in Tyden magazine after Frantisek Nachtigall's becoming editor in chief tries to desribe changes in the new weekly Tyden between the beginning od June, 2008, and the end of May, 2010. There were three different editors in chief in this two years long time period. Dalibor Balsinek had been working at the top position till the end of January, 2009. After that he was temporarily replaced by Daniel Malek. At the end of September, 2010, Frantisek Nachtigall became the editor in chief. The thesis focuses on changes that Nachtigall made. He came to Tyden from a tabloid news paper Aha!, therefore some people were worried that Tyden could become more tabloids like. The author of this thesis tries to find out whether their assumptions were right. After the presenting of the Tyden weekly and the three editor in chief figures the thesis brings a description of the changes in each section and subsection. It focuses on the structural and personal changes, variations of the front page and differences in the choice of topics
Changes in Tyden magazine after Frantisek Nachtigalls editor in chief
No full textThe bachelor's thesis Changes in Tyden magazine after Frantisek Nachtigall's becoming editor in chief tries to desribe changes in the new weekly Tyden between the beginning od June, 2008, and the end of May, 2010. There were three different editors in chief in this two years long time period. Dalibor Balsinek had been working at the top position till the end of January, 2009. After that he was temporarily replaced by Daniel Malek. At the end of September, 2010, Frantisek Nachtigall became the editor in chief. The thesis focuses on changes that Nachtigall made. He came to Tyden from a tabloid news paper Aha!, therefore some people were worried that Tyden could become more tabloids like. The author of this thesis tries to find out whether their assumptions were right. After the presenting of the Tyden weekly and the three editor in chief figures the thesis brings a description of the changes in each section and subsection. It focuses on the structural and personal changes, variations of the front page and differences in the choice of topics
The IKEM Pancreas and Islet Transplant Program as Part of Healthcare for Type 1 Diabetes Patients: Retrospective Analysis of Outcome from 1983 to 2010
No full text13th World Congress of the International Pancreas and Islet Transplant Association (IPITA)
No full text
An Application of the DEDS Control Synthesis Method
No full textAn application of the method suitable for modelling and control of general discrete event dynamic systems (DEDS) to special kinds of communication systems is presented in this paper. The approach is based on Petri nets (PN) defined in [Peterson 1981] and directed graphs (DG) described e.g. in [Diestel 1997]. It is supported by the previous author s works, especially [Capkovic 2003], [Capkovic 1998], [Tzafestas and Capkovic 1997]
Imaging the Beta-cell mass:Why and How
No full textUdgivelsesdato: 2008 SpringDiabetes is a disorder characterized by beta-cell loss or exhaustion and insulin deficiency. At present, knowledge is lacking on the underlying causes and for the therapeutic recovery of the beta-cell mass. A better understanding of diabetes pathogenesis could be obtained through exact monitoring of the fate of beta-cells under disease and therapy conditions. This could pave the way for a new era of intervention by islet replacement and regeneration regimens. Monitoring the beta-cell mass requires a reliable method for noninvasive in vivo imaging. Such a method is not available at present due to the lack of a beta-cell-specific contrast agent. The only existing method to monitor islet cells in vivo consists of labeling islet transplants with iron nanoparticles prior to transplantation and visualization of the transplanted islets by magnetic resonance imaging (MRI). Therefore, accurate assessment of the native beta-cell mass is still limited to autopsy studies. Endeavors to find a biological structure specific for beta-cells led to the discovery of potential candidates that have been tested for noninvasive imaging. Among them are the ligand to the vesicular monoamine transporter type 2 (VMAT-2), which is called dihydrotetrabenazine (DTBZ), antibodies to zinc transporter (ZnT-8) and the monoclonal antibody IC2. While DTBZ and antibodies to ZnT-8 showed binding activities to more than beta-cells, the anti-IC2 monoclonal antibody showed binding properties exclusively to insulin-producing beta-cells. This effect was demonstrated in many previous investigations, and has been further substantiated more recently. Thus, at present, IC2 seems to be the only useful marker for noninvasive functional imaging of native beta-cells. Experiments with a radioisotope-chelated IC2 structure on pancreas ex vivo showed that the tracer specifically bound to the beta-cell surface and could be detected by nuclear imaging. In the near future, these promising findings may offer a new way to monitor the beta-cell mass in vivo under disease and therapy conditions so that we can learn more about diabetes pathogenesis and options for disease prevention
Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors
No full textDirect reprogramming of pancreatic nonendocrine cells into insulin-producing β-cells represents a promising approach for the treatment of insulin-dependent diabetes. However, its clinical application is limited by the potential for insertional mutagenesis associated with the viral vectors currently used for cell reprogramming. With the aim of developing a nonintegrative reprogramming strategy for derivation of insulin-producing cells, here, we evaluated a new approach utilizing synthetic messenger RNAs encoding reprogramming transcription factors. Administration of synthetic mRNAs encoding three key transcription regulators of β-cell differentiation'Pdx1, Neurogenin3, and MafA'efficiently reprogrammed the pancreatic exocrine cells into insulin-producing cells. In addition to the insulin genes expression, the synthetic mRNAs also induced the expressions of genes important for proper pancreatic β-cell function, including Sur1, Kir6.2, Pcsk1, and Pcsk2. Pretreating cells with the chromatin-modifying agent 5-Aza-2′-deoxycytidine further enhanced reprogramming efficiency, increasing the proportion of insulin-producing cells from 3.5 ± 0.9 to 14.3 ± 1.9% (n = 4). Moreover, 5-Aza-2′-deoxycytidine pretreatment enabled the reprogrammed cells to respond to glucose challenge with increased insulin secretion. In conclusion, our results support that the reprogramming of pancreatic exocrine cells into insulin-producing cells, induced by synthetic mRNAs encoding pancreatic transcription factors, represents a promising approach for cell-based diabetes therapy
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