1,721,491 research outputs found
MiRNAs controlling developmental angiogenesis and vascular maturation in zebrafish
No full textMicroRNAs (e.g. miRNAs), endogenously expressed small non- coding RNAs, control gene expression by regulating target messenger RNA at post-transcriptional level. Recent studies have identified a specific role for microRNAs as key regulators of vascular and smooth muscle function and. The zebrafish (Danio rerio) system is a useful model system to study endothelial cell homeostasis during normal and pathological conditions. We describe how the zebrafish model is becoming a useful tool to study the role of microRNAs and their target genes in endothelial and smooth muscle cells during development. Bymanipulating specific miRNAs in zebrafish we demonstrated their functions during cardiovascular development
European Perspectives in Cardiology.Funding: Human Frontier Science Program
Full text linkDr Santoro received an HFSP Career Development Award in 2008. It provided 3 years of funding for salaries, instruments, and reagents/disposables, which enabled him to set up his own lab at the University of Torino. His lab uses the zebrafish model system to identify new molecular mechanisms and pathways involved in the formation and maturation of blood vessels, particularly focusing on endothelial cells and vascular mural cells. Recently, they described vascular smooth muscle cells in the zebrafish during evolution, and they characterised, in collaboration with another lab, the role of catepsin D during zebrafish development. They have developed new techniques to study microRNA functions in the cardiovascular system in zebrafish, and, in collaboration with Pascal Meier, MD, in London, England, they are further characterising the vertebrate cIAP gene. “Before obtaining the Career Development Award, I had been working on cloning new zebrafish mutants with specific cardiovascular development defects,” says Dr Santoro. “I identified the tomato mutant, a null mutant of cIAP1 with specific apoptotic defects in endothelial cells. Later on, I was also involved in the characterisation of microRNA functions in the zebrafish cardiovascular system, in particular microRNA-126.
The admiR-able advances in cardiovascular biology through the zebrafish model system
Full text linkMicroRNAs are small non-coding RNAs endogenously expressed by all tissues during development and adulthood. They regulate gene expression by controlling the stability of targeted messenger RNA. In cardiovascular tissues microRNAs play a role by modulating essential genes involved in heart and blood vessel development and homeostasis. The zebrafish (Danio rerio) system is a recognized vertebrate model system useful to study cardiovascular biology; recently, it has been used to investigate microRNA functions during natural and pathological states. In this review, we will illustrate the advantages of the zebrafish model in the study of microRNAs in heart and vascular cells, providing an update on recent discoveries using the zebrafish to identify new microRNAs and their targeted genes in cardiovascular tissues. Lastly, we will provide evidence that the zebrafish is an optimal model system to undercover new microRNA functions in vertebrates and to improve microRNA-based therapeutic approaches
La genetica nella gestione dei tumori della tiroide: endocrinologo e genetista a confronto
No full textbasi molecolari dei carcinomi tiroide
Sviluppo e caratterizzazione di inibitori di proteine di fusione oncogenetiche quali farmaci innovativi in oncoematologia.
No full textMolecular targets in differentiated and medullary thyroid cancers
No full textMolecular basis of novel treatment modalities for thyroid cance
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