1,720,962 research outputs found
Chlorhexidine gel for control of subgingival bacterial plaque: experimental microbiological evaluation
No full textThe Authors studies effects of chlorexidine gel 1% on subgingival microbial flora in a group of periodontal patients. Microbial findings appear to demonstrate some activity of chlorexidine gel 1%
Human monocytes and gingival fibroblasts release tumor necrosis factor-alpha, interleukin-1 alpha and interleukin-6 in response to particulate and soluble fractions of Prevotella melaninogenica and Fusobacterium nucleatum
No full textIn this study we provide evidence that structural and soluble components of periodontopathogenic bacteria, such as Prevotella melaninogenica and Fusobacterium nucleatum, induce the release of cytokines in vitro known to cause in vivo necrotic inflammatory phenomena and bone resorption (tumor necrosis factor-α, interleukin-1α and interleukin-6). Human monocytes and gingival fibroblasts were cultivated in vitro in the presence of both particulate and soluble bacterial fractions. A dose-dependent production of tumor necrosis factor-α by monocytes and gingival fibroblasts was observed in the presence of fractions of P. melaninogenica and F. nucleatum. Interleukin-1α was produced in approximately the same quantities in the presence of soluble fractions of either P. melaninogenica or F. nucleatum, but in greater quantities in response to particulate fractions of P. melaninogenica. Monocytes release larger amounts of interleukin-1α (about 3000 pg/ml) than gingival fibroblasts (about 1500 pg/ml). Interleukin-6 was released in greater quantities by monocytes in the presence of the pellet fraction of P. melaninogenica (about 5.5 ng/ml), but gingival fibroblasts released larger amounts of interleukin-6, especially in the presence of particulate and soluble components of F. nucleatum (about 12 ng/ml). The ability to induce the release of these cytokines notably increases the pathogenic potential of the bacteria involved in the damage of periodontal tissue
Neutrophil function and microbial associations in rapidly progressing periodontitis and chronic periodontitis in the adult
No full textOur study was focused on the functional characteristics of neutrophil leukocytes (PMN) and the subgingival microflora in two different forms of periodontal disease: 1) adult periodontitis (AP); 2) rapidly progressive periodontitis (RPP). Our study dealt with the functional characteristics of neutrophil leukocytes in the gingival fluid and in the peripheral blood. These were found markedly reduced in the RPP group, while, in the AP group, they were comparable to those of a healthy control group. No difference between local and systemic values was detected. Moreover, some samples of subgingival plaque were taken from two groups of patients, affected by AP and RPP respectively. The above samples showed a predominance of Gram-negative flora over Gram-positive flora, and of anaerobic flora over the aerobic one, and the predominance of specific pathogens in each of the two forms of periodontal disease. The subgingival plaque samples taken at the end of the periodontal treatment from five out of ten patients affected by RPP showed inverse ratios, as well as the absence of the previously detected pathogens. The findings underline the relevance of tests of leukocytes functionality and that of microbiological analysis to allow correct diagnosis of dubious forms of periodontal disease and the checking of the posttreatment results
Studio sperimentale sugli effetti della clorexidina cimentata in vitro con colture cellulari di fibroblasti del legamento parodontale umano Nota I
No full textStudio sperimentale sugli effetti della clorexidina cimentata in vitro con colture cellulari di fibroblasti del connettivo gengivale umano . Nota II
No full textStudio della risposta immunitaria alla vaccinazione antiepatite B in pazienti affetti da diabete mellito tipo 1 al momento della prima copertura universale in Campania
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