236 research outputs found

    DNA ニンシキ ブンシ ナラビニ DNA ヒカリ セツダン ブンシ ノ デザイン

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    京都大学0048新制・課程博士博士(工学)甲第8974号工博第2065号新制||工||1209(附属図書館)UT51-2001-F304京都大学大学院工学研究科合成・生物化学専攻(主査)教授 齋藤 烈, 教授 北川 進, 教授 今中 忠行学位規則第4条第1項該当Doctor of EngineeringKyoto UniversityDA

    Protected Culture for Vegetable and Small Fruit Crops: Types of Structures

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    A protective structure is defined as any structure designed to modify the environment in which plants are grown. Protective structures, such as greenhouses, screen houses, and tunnels, are known worldwide as production systems for high-quality vegetable and fruit crops.  This minor revision by Shinsuke Agehara adds updated information regarding 2015 revisions to the Worker Protection Standards in the last paragraph, and removes one former author no longer affiliated with UF. https://edis.ifas.ufl.edu/hs1224 Previous version: Santos, Bielinski, Gary Vallad, and Emmanuel Torres-Quezada. 2013. “Protected Culture for Vegetable and Small Fruit Crops: Types of Structures”. EDIS 2013 (7). https://journals.flvc.org/edis/article/view/121080

    Correction to: Comparison of glycyrrhizin content in 25 major kinds of Kampo extracts containing Glycyrrhizae Radix used clinically in Japan

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    The article Comparison of glycyrrhizin content in 25 major kinds of Kampo extracts containing Glycyrrhizae Radix used clinically in Japan, written by Mitsuhiko Nose, Momoka Tada, Rika Kojima, Kumiko Nagata, Shinsuke Hisaka, Sayaka Masada, Masato Homma and Takashi Hakamatsuka, was originally published Online First without open access. After publication in volume 71, issue 4, page 711–722 the author decided to opt for Open Choice and to make the article an open access publication. Therefore, the copyright of the article has been changed to © The Author(s) 2018 and the article is forthwith distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.</jats:p

    Probing renal pH using hyperpolarized [1-13C]alaninamide

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    Hyperpolarized molecular probes can be effectively used as pH markers. To date, the only two probes reported in vivo as extracellular pH sensors are 13C-bicarbonate, and [1,5-13C2]zymonic acid. Alaninamide is a derivative of alanine which is found to be sensitive to variations of pH in the physiological range. The aim of the present study was to assess the feasibility of using alaninamide as a pH probe in vivo. The alaninamide titration curve was determined by performing 13C NMR measurements at 9.4 T, 37° on a set of 500 mM Ala-NH2∙HCl samples of varying pH referenced to 13C urea. [1-13C]Alaninamide was polarized at 1 K in a 7 T polarizer, then rapidly dissolved in a buffered solution and injected IV into a Sprague Dawley rat (n=6) located in a 9.4 T animal scanner. 13C FIDs were acquired with 30° BIR4 pulses using a single loop 1H / quadrature 13C surface coil placed over the left kidney. The pH was perturbed by injecting acetazolamide IV (10 mg/kg) one hour prior to infusion. The alaninamide titration curve shows a 13C1 chemical shift change of ≈ 8.4 ppm, and a pKa of 7.9. The pH sensitivity of 13C1 results in three distinct alaninamide spectral peaks, corresponding to three different extracellular pH compartments within the kidney (pH = 7.46, pH = 7.22, pH = 6.58) that can be tentatively assigned to the cortex/blood, medulla and calyx/ureter. With acetazolamide treatment, the pH in the first compartment follows the change in pH of the blood, while the pH in the third compartment does not reflect the urine pH and shifts during the brief experiment. No change is observed in the pH value of the second compartment.LIFMET2018, University of Southampton, A. Radaelli, R. Hata, S. Sando, O. Bonny, A. Comment R. Gruetter, H. A. I. Yoshihara. Oral presentation, last author Alice Radaeli T13, P7
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