39 research outputs found
Effect of Some Design Parameters on Performance of a Shutter Type Vertical Axis Wind Turbine
No full textMulti-Independent Optimization while Turning of Inconel-600 alloy using Grey Interactive Exploration
No full textThis investigation effort offers multi-quality attributes optimization while turning of Inconel-600 superalloy. Taguchi's L9 orthogonal planning is implemented to review the upshot of governing aspects such as machining speed, feed rate, and depth of cut on vibrations and surface roughness (SR). To heighten all the three leading variables, the grey interactive exploration (GIE) is implemented. The grey interactive rating (GIR) is practiced as a multi-quality exclusive key (MQEK). The finest formation of central variables acquired from the investigational grades is cutting speed 500 m/min, feed rate 0.22 mm/rev and depth of cut 0.5 mm. ANOVA scrutiny signposts that feed rate is a crucial variable relating to the superiority yields. Products of endorsement pilots display that the ideal foremost variables developed the grey interactive rating from 0.6932 to 0.8138 for the numerous retorts. Scanning Electron Microscopic (SEM) scrutiny of cutting tool spectacles that fracture, chipping, abrasion and adhesion are the primary wear phenomena
Theoretical Analysis of Composite Blade in Static Condition to Find Material Uncertainty and Validation with COMSOL Software
No full textDiurnal rhythm of a unicellular diazotrophic cyanobacterium under mixotrophic conditions and elevated carbon dioxide
No full textMixotrophic cultivation of cyanobacteria in wastewaters with flue gas sparging has the potential to simultaneously sequester carbon content from gaseous and aqueous streams and convert to biomass and biofuels. Therefore, it was of interest to study the effect of mixotrophy and elevated CO2 on metabolism, morphology and rhythm of gene expression under diurnal cycles. We chose a diazotrophic unicellular cyanobacterium Cyanothece sp. ATCC 51142 as a model, which is a known hydrogen producer with robust circadian rhythm. Cyanothece 51142 grows faster with nitrate and/or an additional carbon source in the growth medium and at 3 % CO2. Intracellular glycogen contents undergo diurnal oscillations with greater accumulation under mixotrophy. While glycogen is exhausted by midnight under autotrophic conditions, significant amounts remain unutilized accompanied by a prolonged upregulation of nifH gene under mixotrophy. This possibly supports nitrogen fixation for longer periods thereby leading to better growth. To gain insights into the influence of mixotrophy and elevated CO2 on circadian rhythm, transcription of core clock genes kaiA, kaiB1 and kaiC1, the input pathway, cikA, output pathway, rpaA and representatives of key metabolic pathways was analyzed. Clock genes’ transcripts were lower under mixotrophy suggesting a dampening effect exerted by an external carbon source such as glycerol. Nevertheless, the genes of the clock and important metabolic pathways show diurnal oscillations in expression under mixotrophic and autotrophic growth at ambient and elevated CO2, respectively. Taken together, the results indicate segregation of light and dark associated reactions even under mixotrophy and provide important insights for further applications
Numerical and experimental investigations on the performance of a serpentine microchannel with semicircular obstacles
No full textParametric Optimization for Photochemical Machining of Copper Using Grey Relational Method
No full textParametric Optimization for Photochemical Machining of Copper using Overall Evaluation Criteria
No full textInfluence of mixotrophic growth on rhythmic oscillations in expression of metabolic pathways in diazotrophic cyanobacterium Cyanothece sp ATCC 51142
No full textThis study investigates the influence of mixotrophy on physiology and metabolism by analysis of global gene expression in unicellular diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 ( henceforth Cyanothece 51142). It was found that Cyanothece 51142 continues to oscillate between photosynthesis and respiration in continuous light under mixotrophy with cycle time of similar to 13 h. Mixotrophy is marked by an extended respiratory phase compared with photoautotrophy. It can be argued that glycerol provides supplementary energy for nitrogen fixation, which is derived primarily from the glycogen reserves during photoautotrophy. The genes of NDH complex, cytochrome c oxidase and ATP synthase are significantly overexpressed in mixotrophy during the day compared to autotrophy with synchronous expression of the bidirectional hydrogenase genes possibly to maintain redox balance. However, nitrogenase complex remains exclusive to nighttime metabolism concomitantly with uptake hydrogenase. This study throws light on interrelations between metabolic pathways with implications in design of hydrogen producer strains. (C) 2015 Elsevier Ltd. All rights reserved
Rhythmic oscillations in KaiC1 phosphorylation and ATP/ADP ratio in nitrogen-fixing cyanobacterium <i>Cyanothece</i> sp. ATCC 51142
No full text<p>Cyanobacterial circadian clock composed of the Kai oscillator has been unraveled in the model strain <i>Synechococcus elongatus</i> PCC 7942. Recent studies with nitrogen-fixing <i>Cyanothece</i> sp. ATCC 51142 show rhythmic oscillations in the cellular program even in continuous light albeit with a cycle time of ~11 h. In the present study, we investigate correlation between cellular rhythms, KaiC1 phosphorylation cycle, ATP/ADP ratio, and the redox state of plastoquinone pool in <i>Cyanothece</i>. KaiC1 phosphorylation cycle of <i>Cyanothece</i> was similar to that of <i>Synechococcus</i> under diurnal cycles. However, under continuous light, the cycle time was shorter (11 h), in agreement with physiological and gene expression studies. Interestingly, the ATP/ADP ratio also oscillates with an 11 h period, peaking concomitantly with the respiratory burst. We propose a mathematical model with C/N ratio as a probable signal regulating the clock in continuous light and emphasize the existence of a single timing mechanism regardless of the cycle time.</p
Experimental Analysis of Louvered Rectangular Leaf Type Inserts in a Circular Pipe to Enhance Heat Transfer Coefficient
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