4 research outputs found

    Could β-hemolytic, group B Enterococcus faecalis be mistaken for Streptococcus agalactiae?

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    AbstractA β-hemolytic Enterococcus faecalis strain agglutinating Lancefield group A, B, C, D, F, and G antisera was observed from a rectovaginal swab, in the context of antenatal screening for Streptococcus agalactiae (group B Streptococcus [GBS]). This is the first multi-Lancefield antisera-agglutinating isolate of this species, and it raised particular concern, as it may mimic GBS, leading to false reporting and useless receipt of intrapartum antibiotics

    Impact of Salmonella enteritidis Infection and Mechanical Stress on Antimicrobial Peptide Expression in Hermetia illucens

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    Hermetia illucens, the black soldier fly, is a common and widespread fly of the family Stratiomyidae. Its ability to grow on contaminated substrates suggests the production of antimicrobial peptides that enable its survival. This study aimed to verify the impact of direct and indirect infection with Salmonella enteritidis on the expression of defensins and cecropins in Hermetia illucens larvae. In addition to an infection with a microorganism, it was interesting to verify if the expression of peptides and the relative action of hemolymph changed in larvae subjected to mechanical stress by abdominal puncture. The peptide fraction of the hemolymph of infected larvae was tested using antibiogram and minimum inhibitory concentration tests against Salmonella enteritidis and Salmonella typhimurium. Both molecular and microbiological tests were carried out at three different time points, on larvae not subjected to any treatment (T-0), four hours after treatment (T-1), and 24 h after treatment (T-2). The results of the microbiological tests showed the antimicrobial action of the peptide fraction of the hemolymph against both S. typhimurium and S. enteritidis; for the latter one, the action was more marked. Interesting results were also found for larvae subjected only to mechanical stress by puncture. Molecular tests on the expression of defensins and cecropins were in full agreement with those obtained in the microbiological tests, with expression more pronounced in larvae infected directly with Salmonella enteritidis. Temporal and condition-specific regulation of defensins and cecropins highlights the complexity of the immune response and suggests sophisticated mechanisms by which the host fine-tunes antimicrobial peptide expression to enhance pathogen defense while preventing excessive immune activation

    Retrospective analysis of animal poisoning in the province of Viterbo, Italy

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    Background: Intentional poisoning represents a serious health risk for domestic and wild animals, this phenomenon has been widespread in Italy for several years. Aim: Our study aims to examine data on animal poisonings that occurred in the province of Viterbo, Lazio region located in central Italy, from 2003 to 2017. The aim of this study is to provide data on the poisoning of animals in the province of Viterbo. Methods: A total of 1078 cases of suspected poisoning were analysed during the period under review. Of these, 761 (70.6%) were carcasses, 189 (17.6%) were baits, 107 (9.9%) were gastric contents, and 21 (1.9%) were samples of various origins. Results: A total of 631 cases (58.5%) were confirmed as involving toxic substances. Zinc phosphide (34.7%) was the most prevalent cause of poisoning, followed by metaldehyde (18.7%), a coumarin rodenticide (12.7%), other molecules were also identified with lower percentages. The animal species most frequently involved in poisoning are dogs (66.2%), followed by cats (25.5%), birds (4.9%), while wild animals, ruminants and fish represent a small percentage of animals involved. Conclusion: This phenomenon represents a significant threat to animals, humans and the environment. Therefore, it is essential to implement effective measures to combat this issue. [Open Vet J 2024; 14(12.000): 3241-3247
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