586 research outputs found
Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain
No full textCastro, David J., Cerezo, Isabel, Sampedro, Inmaculada, Martínez-Checa, Fernando (2018): Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain. International Journal of Systematic and Evolutionary Microbiology 68 (6): 1851-1856, DOI: 10.1099/ijsem.0.002744, URL: http://dx.doi.org/10.1099/ijsem.0.00274
Roseovarius ramblicola Castro & Cerezo & Sampedro & Martínez-Checa 2018, SP. NOV.
No full textDESCRIPTION OF ROSEOVARIUS RAMBLICOLA SP. NOV. Roseovarius ramblicola [ram.bli′ co.la. Spanish fem. n. rambla sandy ground; L. suff. -cola (from L. masc. or fem. n. incola) inhabitant; N.L. n. ramblicola inhabitant of a rambla]. Cells are ovoid- or rod-shaped, Gram-stain-negative, nonmotile, 0.7–1.2×2.1–3.2 µm in size and reproduce by budding or asymmetrical division. Cell colonies are white, circular, convex and opaque when grown on MA and R2A media. The growth pattern is uniform in a liquid medium. Capable of growing in NaCl concentrations of 0.5 to 10 % (w/v), with optimum growth occurring at 3 %. Grows within a temperature range of 5–30 Ǫ C at pH values of between 6 and 9, the optimum values being 28 Ǫ C and pH 7. Catalase and oxidase are produced. Nitrate reduction, nitrite reduction, indol production, hydrolysis of arginine, aesculin and gelatine are negative. Urease is not produced. In API 20NE assays, assimilation of D- glucose, maltose, L- arabinose, mannose, N -acetyl-b- glucosamine, gluconate, adipic acid, malic acid, phenylacetic acid, capric acid and trisodium citrate are negative. In API ZYM assays, leucine arylamidase is positive, but esterase C4, esterase lipase C8, naphthol-AS-BIphosphohydrolase and alkaline phosphatase lipase C14, valine arylamidase, cystine arylamidase, trypsine, a- chymotrypsin, a- galactosidase, b- galactosidase, b- glucuronidase, a- glucosidase, b- glucosidase, N -acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase are negative. Acid is not produced from sugar in API 50 CH. It is susceptible to amoxicillin, amoxicillin/clavulanic acid, ampicillin, azithromycin, aztreonam, bacitracin, carbenicillin, cefotaxime, cefalotin, cefoxitin, cefuroxime, chloramphenicol, erythromycin, rifampicin, penicillin, tobramycin, trimethoprim/ sulfamethoxazole, norfloxacin, sulfonamides and streptomycin. Resistant to gentamicine, nalidixic acid, polymyxin B, ciprofloxacin, tetracyclin, amikacin and neomycin. The only detected lipoquinone is ubiquinone with ten isoprene units (Q10). The DNA G+C content of the type strain is 63 mol% (Tm method). The major fatty acids (>5 % of the total fatty acids) of strain D15 T are C 18: 1 Ɯ 7 c, C16: 0 and C12: 0. The polar lipids contain phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three unidentified polar lipids. The type strain, D15 T (= CECT 9424 = LMG 30322), was isolated from a saline soil sample from Rambla Salada (Murcia) south-eastern Spain.Published as part of Castro, David J., Cerezo, Isabel, Sampedro, Inmaculada & Martínez-Checa, Fernando, 2018, Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain, pp. 1851-1856 in International Journal of Systematic and Evolutionary Microbiology 68 (6) on page 1855, DOI: 10.1099/ijsem.0.002744, http://zenodo.org/record/622371
Solid-state cultures of Fusarium oxysporum transform aromatic components of olive-mill dry residue and reduce its phytotoxicity
No full textThe present study mainly investigated the ability of solid-state cultures of the non-pathogenic Fusarium oxysporum strain BAFC 738
to transform aromatic components to reduce the phytotoxicity in olive-mill dry residue (DOR), the waste from the two-phase manufac- turing process. Lignin, hemicellulose, fats and water-soluble extractives contents of DOR colonized by the fungus for 20 weeks were reduced by 16%, 25%, 71% and 13%, respectively, while the cellulose content increased by 25%. In addition, the ethyl acetate-extractable phenolic fraction of the waste was reduced by 65%. However, mass-balance ultra-Wltration and size-exclusion chromatography experi- ments suggested that the apparent removal of that fraction, mainly including 2-(3,4-dihydroxyphenyl)ethyl alcohol and 2-(4-hydroxyphe- nyl)ethyl alcohol, was due to polymerization. Mn-peroxidase and Mn-independent peroxidase activities were found in F. oxysporum solid-state cultures, while laccase and aryl alcohol oxidase activities were not detected. Tests performed with seedlings of tomato (Lyco- persicum esculentum L.), soybean (Glycine maximum Merr.), and alfalfa (Medicago sativa L.) grown on soils containing 6% (w/w) of bio- converted DOR (kg soil)¡1 showed that the waste’s phytotoxicity was removed by 20 weeks-old fungal cultures. By contrast, the same material exhibited a high residual toxicity towards lettuce (Lactuca sativa L.)The present study mainly investigated the ability of solid-state cultures of the non-pathogenic Fusarium oxysporum strain BAFC 738 to transform aromatic components to reduce the phytotoxicity in olive-mill dry residue (DOR), the waste from the two-phase manufacturing process. Lignin, hemicellulose, fats and water-soluble extractives contents of DOR colonized by the fungus for 20 weeks were reduced by 16%, 25%, 71% and 13%, respectively, while the cellulose content increased by 25%. In addition, the ethyl acetate-extractable phenolic fraction of the waste was reduced by 65%. However, mass-balance ultra-filtration and size-exclusion chromatography experiments suggested that the apparent removal of that fraction, mainly including 2-(3,4-dihydroxyphenyl)ethyl alcohol and 2-(4-hydroxyphenyl)ethyl alcohol, was due to polymerization. Mn-peroxidase and Mn-independent peroxidase activities were found in F. oxysporum solid-state cultures, while laccase and aryl alcohol oxidase activities were not detected. Tests performed with seedlings of tomato (Lycopersicum esculentum L.), soybean (Glycine maximum Merr.), and alfalfa (Medicago sativa L.) grown on soils containing 6% (w/w) of bioconverted DOR (kg soil)-1 showed that the waste's phytotoxicity was removed by 20 weeks-old fungal cultures. By contrast, the same material exhibited a high residual toxicity towards lettuce (Lactuca sativa L.). © 2006 Elsevier Ltd. All rights reserved
Libro de ingresos y egresos de los fondos de Providencias y de las Ánimas del Purgatorio
No full textLibro de ingresos y egresos de los fondos de Providencias y de las Ánimas del Purgatorio, 190
Libro 2 de ingresos y egresos de cera así como los de efectivo que corresponden al fondo de la divina providencia cuya hermandad se estableció el mes de noviembre de 1915
No full textLibro 2 de ingresos y egresos de cera así como los de efectivo que corresponden al fondo de la divina providencia, cuya hermandad se estableció el mes de noviembre de 191
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