33 research outputs found
Protective effects of Aristolochia longa and Aquilaria malaccensis against lead induced acute liver injury in rats
No full textObjective: To investigate the protective effects of Aristolochia longa (A. longa) and Aquilaria malaccensis (A. malaccensis) on acute hepatotoxicity induced by lead in female albino rats.Methods: Twenty five (25) apparently healthy female Wistar rats were randomly divided into five groups of five rats in each: control, Pb, Pb + A. longa (Ar), Pb+ A. malaccensis (Aq), and Pb+ A. longa (Ar) + A. malaccensis (Aq) lead (100 mg/kg b.w.) as Pb (C2H3O2)2 added in their drinking water for 75 days. A. longa (rhizome powder at a dose of 10 g/kg of diet) and A. malaccensis (heartwood powder at a dose 10 g/kg of diet) were added to the feed during the last 15 days of lead exposed in the animals.Results: Obtained results revealed that lead treatment caused a significant increase in serum GOT, GPT and ALP activities and in liver of MDA level and CAT activity. In contrast, it led to an decrease in the liver GOT, GPT and GST activities and in GSH level in rats. Also, the results clearly showed that lead causes alterations of hepatic tissue in comparison with controls. Our results showed that treatment with A. malaccensis and A. longa a partial correction of the previous parameters. The histological observations confirmed the hepatoprotection results by the biochemical parameters.Conclusions: Results demonstrated beneficial effects of A. longa and A. malaccensis treatment in Pb-induced oxidative stress and tissue damage in liver
Protective effect of zinc and Pine barks extract supplement against Indometacin-induced gastric ulcer in rats
No full textComparative study of immobilized enzyme on nano-composite (SCN) and free enzyme of invertase isolated from baker\u27s yeast
No full textThe objective of this study was to compare the properties and performance of invertase enzyme isolated from baker\u27s yeast, both in free and immobilized form on a starch-copper nanocomposite (SCN). The SCN was synthesized using starch as a reducing agent for the biological production of copper nanoparticles (CuNPs). The Characterization of SCN was performed using Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction to confirm nanoparticle formation and structural properties. The immobilization of invertase onto SCN was optimized by varying nanoparticle concentration, pH, incubation time, and temperature to maximize enzyme attachment and activity. Enzyme activity was measured for both free and immobilized forms to determine the immobilization efficiency. The study found that the high levels of enzyme immobilization were observed at pH = 9, temperature T = 30, and 3% SCN concentration. For both free and immobilized invertase, the ideal reaction temperatures were 35°C and 40°C, with corresponding pH values of 5 and 4.5. Reusability experiments revealed that the immobilized enzyme retained 49% of its activity after ten cycles, demonstrating improved stability and potential for repeated use. The results suggest that enzyme immobilization on SCN occurs through non-covalent interactions, providing a practical and sustainable approach for biocatalytic applications. This research highlights the potential of starch-based nanocomposites for enzyme stabilization, offering a cost-effective and environmentally friendly solution for industrial and biotechnological applications
STUDY OF THE EFFECT OF OBESITY ON IMMUNE CELLS ACTIVITY AND CARDIOVASCULAR DISEASE RISK IN EL-OUED (ALGERIA) WOMEN
No full textObjective: This study was conducted to find out the effect of obesity on immune cells activity and some biochemical markers in women.
Methods: The experimentation is carried out on 30 voluntary individuals were divided into two groups; 15 women with obesity (Body Mass Index [BMI]>30) were selected, On the other hand, we took 15 witnesses (BMI<30). All the volunteers were subjected to estimate the lipid profile, biochemical, and hematological parameters.
Results: Results obtained show that there was a significant increase of blood glucose (p<0.05), triglyceride (p<0.05) and uric acid (p<0.001) and no significant change of other biochemical markers in obese people’s group when compared to the control subjects. In addition, the hematological results demonstrated that there was a significant decrease (p<0.05) of lymphocytes, hemoglobin, hematocrit, and mean corpuscular volume levels (p<0.01) in women with obesity compared to the controls.
Conclusion: Our study reveals that the obesity may be induce the sever alteration in lipid profile which cause the cardiovascular risk for this population. In addition, the obesity causes a remarkable change in some biochemical markers and blood constituent’s anemia or other metabolic disorder in the body
Efficient Up-Conversion ZnO Co-Doped (Er, Yb) Nanopowders Synthesized via the Sol-Gel Process for Photovoltaic Applications
No full textIn this study, undoped and (Erbium, Ytterbium) co-doped ZnO nanopowders were prepared using the sol-gel method and the supercritical drying of ethyl alcohol. Doping ZnO nanopowders were elaborated with 5 mol% of Er (5 Er: ZnO), 5 mol% of Er and 5 mol% of Yb (5 Er, 5 Yb: ZnO), and 5 mol% of Er and 10 mol% of Yb (5 Er, 10 Yb: ZnO) concentrations. The effects of the Yb concentration on the structural, morphological, photoluminescent, and electrical properties of the ZnO nanopowders were investigated. The main findings of this work were the crystallinization of all of the nanopowders in a hexagonal Wurtzite structure with a spheroidal morphology and a size of 60 nm. Hence, the doping concentration would affect the crystallinity and the morphology of the ZnO nanopowder. The UC (Up-Conversion) emissions were investigated under a 980 nm excitation. It was observed that (Er, Yb: ZnO) exhibited green, ranging between 525 nm and 550 nm and red up-converted emissions of 655 nm, due to the efficient energy transfer process between Er3+ and Yb3+. The absolute quantum yield percentage (QY %) of the doped nanopowders was measured as a function of power density at each up-converted emission. This would prove that (5 Er, 5 Yb: ZnO) had the highest QY percentage value of 6.31 ± 0.2% at a power density of 15.7 W/cm2. Additionally, it had the highest excited state lifetime for green and red emissions. Moreover, the Hall effect measures showed that the resistivity decreased while the electron mobility increased after doping, suggesting that most of rare earth ions were located in the interstitial positions. The carrier concentration increased after doping until (5 Er, 5 Yb: ZnO), suggesting that the Zn2+ ions substituted the RE3+ ions. Then, the carrier concentration decreased, suggesting that doping with higher concentrations would cause grain boundary defects. These findings would suggest that (5 Er, 5 Yb: ZnO) would have the best electrical properties and the lowest band gap energy (3.24 eV). Therefore, the presented preparation of the (Er, Yb: ZnO) nanopowders elaborated, using the sol-gel process would be a potential interesting material for UC applications
Study of Carbonated Clay-Based Phosphate Geopolymer: Effect of Calcite and Calcination Temperature
No full textThis study aims to use natural carbonated Tunisian clay as an aluminosilicate precursor for the elaboration of phosphate-based geopolymers, which yields to the valorization of this common material in Tunisia. In addition, the presence of calcium carbonate in this clay allows the investigation of this associated mineral’s effect on the properties of geopolymeric materials. To achieve these purposes, several experimental techniques were used, namely fluorescence (FX), particle size analysis, thermogravimetric analysis (TGA), differential thermal analysis (DTA), dilatometric analysis, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The mechanical strength and the open porosity of the obtained geopolymeric samples were tested by the compression test and the standard test method for water absorption, respectively. The findings of this work show that the used Tunisian clay can present an attractive aluminosilicate precursor for the synthesis of phosphate-based geopolymers. It also shows that the chosen calcination temperature of the raw clay considerably modifies the reactivity of minerals during geopolymerization and, consequently, strongly affects the properties and structure of the geopolymeric samples. These effects were attributed essentially to the formation of new calcium crystalline phases in the obtained geopolymeric samples. In fact, the anorthite (CaAl2Si2O8) phase appears in all the samples but in greater abundance in those obtained from the clay calcined at 550 °C, and the brushite phase (CaHPO4·2H2O) appears only in the samples obtained from the clay calcined at 950 °C. All these new crystalline phases are strongly dependent on the state of the calcite present in the calcined clay
Assessment of Hematological and Electrolytes Levels and Oxidative Stress Predictive Factors by Logistic Regression Analysis of Acute Ischemic Stroke Disease
No full textBackground: Ischemic stroke is a common disease that affects the blood vessels in the brain. This disease is considered one of the most dangerous ailments that affect the nervous system all over the world.
Objectives: The aim of this study was to evaluate some hematological and biochemical parameters in men with ischemic disease in Touggourt (Algeria) region.
Methods: The study was done on 40 voluntary individuals divided into healthy men reserved as control with Mean±SD age of 56.13±3.32 years and ischemic stroke man (patients) with Mean±SD age of 57.75±4.01 years; their origin covered the whole Touggourt (Algeria) region. Some hematological and biochemical parameters were analyzed, Sensitivity and specificity of oxidative stress biomarkers in serum, erythrocytes, and leucocytes were estimated using a receiver operating characteristics curve (ROC) design.
Results: The results suggest that when compared to the controls, blood glucose levels significantly increased (P>0.05) while serum sodium, potassium, and chloride concentrations and serum alkaline phosphatase activity significantly decreased (P<0.05) in stroke patients. Red blood cell, hemoglobin, glutathione (GSH), and total thiol levels significantly decreased (P<0.05) while white blood cell, neutrophile, platelets, malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (TAC) and vitamine C levels significantly increased (P<0.05) in ischemic stroke patients compared to the controls with high sensitivity and specificity values of oxidative stress markers.
Conclusion: The results indicate that change in electrolytes, hematological, alkaline phosphatase, and oxidative stress markers contributes to the development or complications of ischemic stroke. Investigations on oxidative stress markers can early identify predictors of stroke disease
EFFECT OF EXTRACTS AQUEOUS OF PHRAGMITES AUSTRALIS ON CARBOHYDRATE METABOLISM, SOME ENZYME ACTIVITIES AND PANCREATIC ISLET TISSUE IN ALLOXAN-INDUCED DIABETIC RATS
No full textObjective: The present study was aimed to investigate the effects of the aqueous extract of rhizome of phragmites australis on carbohydrate metabolism, some enzyme activities and pancreatic tissue in alloxan induced diabetic rats.Methods: Wistar rats were divided into three groups (n=6) as control, diabetic group and test groups (Diabetic+AEPA). Diabetes in rats was induced by alloxane using a single peritoneal injection of 150 mg/kg dose. Aqueous extract of Phragmites australis was supplemented (200 mg/kg b. w) orally for three weeks. The aqueous extract of Phragmites australis was prepared and phytochemical were analyzed by using standard methods. Blood glucose level, pancreas histology and various biochemical parameters were assessed.Results: The results of the phytochemical analysis of aqueous extract of Phragmites australis (AEPA) revealed the presence of tannin, terpenoids, glycosides and flavonoids. Compared with the control, a significant decrease in the body weight gain (p<0.01) and increase in food intake (p<0.001) were noticed in the diabetic group. The biochemical evaluation showed significantly higher values for glucose (p<0.001), lipid profile (p<0.05), transaminases (p<0.05), amylase and alkaline phosphatase (p<0.001) activities in diabetic group compared with the control. Histology of the pancreas showed congestion of vessels and focal area of necrosis in diabetic untreated rats. However, treatment with aqueous extract of Phragmites australis significantly normalized blood glucose, serum biochemical profile and pancreas histomorphology.Conclusion: It is suggested that aqueous extract of Phragmites australis exhibits a benefic effect in rat to a great extent in attenuating and restoring the damage sustained by diabetes.</jats:p
