45 research outputs found
A decade of understanding secondary metabolism in Pseudomonas spp. for sustainable agriculture and pharmaceutical applications
No full textASSESSMENT OF ANXIETY AND DEPRESSION LEVEL AMONG PATIENTS OF OPEN-HEART SURGERY (CABG) IN PUNJAB INSTITUTE OF CARDIOLOGY, LAHORE
No full textBackground: Patients are found to suffer from diverse emotional and psychiatric problems after open-heart surgery. However, no clear cut statistics and evidence has been brought to light regarding the incidence and the course of symptoms, cognitive changes, and quality of life among the patients. This study is envisioned to outline the pre- and postoperative psychiatric portfolio, of patients undergoing open heart surgery. Methods: 100 otherwise stable patients prepared for open heart surgery meeting our inclusion criteria were inducted in this study during January 2019 and September 2019. Each patient was assessed on the Mini-Mental State Examination, and the self-structured questionnaire to assess psychiatric symptoms. Patients were assessed before and after 1 months and 6 months interval after the surgery. Results: The level of anxiety and depression was found to be less at 6 months preoperative than the preoperative stage. Conclusions: A gradual symptomatic recovery was noted in the emotional and psychiatric profile over time at follow visits in majority of patients undergoing open heart surgery. Since psychiatric symptoms have a negative impact on patients prognosis and quality of life therefore proper psychiatric consultation before and after cardiac surgeries important to hasten the recovery.
Keywords: Open Heart Surgery, Anxiety, Depression, Mini-Mental State Examinatio
A Healer's Uncertain State of Mind in COVID-19 Pandemic
No full textCurrent plight of COVID-19 in developing countries entails uncertain prognosis, impending severe shortages of resources for testing and treatment and inadequate safety measure for health care providers. The imposition of unfamiliar public health measures that may infringe on personal freedoms, large and growing financial losses, and conflicting messages from authorities are among the other major stressors that undoubtedly will contribute to widespread emotional distress and increased risk for mental fatigue associated with COVID-19.</p
Assessment of anticancer and antimicrobial potential of bioactive metabolites and optimization of culture conditions of pseudomonas aurantiaca PB-St2 for high yields
No full textThe following study aimed to characterize the biological potential of the purified compounds of Pseudomonas aurantiaca PB-St2. Optimization of temperature and incubation time of 32oC and 72 h yielded the highest crude extract weight and optical density of bacterial culture. HPLC analysis of the crude metabolite extract (purified using gravitational column chromatography) showed three fractions named as PC1, PC2, and PC3. HPLC-purified fractions were subjected to LC-MS/MS analysis and the data was compared using reference library. Fraction PC1 was identified as mupirocin, PC2 as phenazine-1-carboxylic acid (PCA), and PC3 as the mixture of three compounds including pyoluteorin, PCA and 2-hydroxyphenazine (2-OH-phz). Fungicidal potential of the purified compounds was assessed against phytopathogens including Fusarium equiseti, Fusarium incarnatum, Alternaria alternata, and Colletotrichum falcatum. Fraction PC3 showed the highest fungicidal activity of ~89%, whereas, the least antifungal activity (~27%) was noted for mupirocin. Antibacterial activity of the purified compounds against Gram-positive pathogen Bacillus cereus, and Gram-negative pathogens Pseudomonas aeruginosa, Salmonella enterica, and Klebsiella oxytoca was also assessed. Fraction PC3 demonstrated the highest antibacterial activity against B. cereus and P. aeruginosa showing 1.8 cm, and 0.9 cm zones of inhibition, respectively. Against K. oxytoca and S. enterica, the antibacterial activity of PB-St2 crude extract was slightly higher than the fraction PC3. The fraction PC3 also demonstrated the highest IC50 against HepG-2 and SF767 cancer cell lines at 25 μg and 20 μg concentrations, respectively. The multifaceted attributes of P. aurantiaca PB-St2 make it an ideal candidate for agricultural and pharmacological applications.Authors would like to acknowledge Alexander Von Humbolt (AvH) Foundation, Bonn-Bad Godesberg (Federal Republic of Germany) for providing equipment grant to Dr. Samina Mehnaz. Mahnoor Zameer was supported by a PhD fellowship by Higher Education Commission (HEC) of Pakistan
Insulinoma - atypical response to tests and uncommon adverse effects to medical treatment
No full textDiversity of Bacillus-like bacterial community in the rhizospheric and non-rhizospheric soil of halophytes (Salsola stocksii and Atriplex amnicola) and characterization of osmoregulatory genes in halophilic Bacilli
No full textSalinity is one of the major abiotic stresses, with a total of 3% of the worldâ s land mass being affected by salinity. Approximately 6.3 million hectares of land in Pakistan is affected by salinity to varying degree and most of the areas are arid to semiarid with low annual precipitation. The aim of present study is to identify and characterize Bacillus and Bacillus-derived bacterial genera from the rhizospheric and non-rhizospheric soil samples from Khewra Salt Mine, Pakistan by using culture-independent as well as culture-dependent methods. Seven Bacillus-like bacterial genera Bacillus, Halobacillus, Virgibacillus, Brevibacillus, Paenibacillus, Tumebacillus and Lysinibacillus were detected by using pyrosequencing analysis whereas only four genera Bacillus, Halobacillus, Oceanobacillus and Virgibacillus were identified by culture-dependent methods. Most of Bacillus-like isolates identified in this study were moderately halophilic, alkaliphilic and mesophilic bacteria and were considered as a good source of hydrolytic enzymes because of their ability to degrade proteins, carbohydrates and lipids. Eight Bacillus-like strains from the genera Bacillus, Halobacillus, Oceanobacillus and Virgibacillus showed positive results for the presence of ectABC gene cluster (ectoine), six strains could synthesize betaine from choline and six strains tested positive for the synthesis of proline from either glutamate or ornithine by using proline dehydrogenase enzyme.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Assessment of two carrier materials for phosphate solubilizing biofertilizers and their effect on growth of wheat (Triticum aestivum L.)
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Mitigation Strategies for Abiotic Stress Tolerance in Plants Through Stress-Tolerant Plant Growth-Promoting Microbes
No full textGeographical distribution of reported cases across Pakistan. (Map is created by author using QGIS software).
No full textGeographical distribution of reported cases across Pakistan. (Map is created by author using QGIS software).</p
