1,721,668 research outputs found
Genotyping Candida species and molecular analysis of C. albicans gene encoding mevalonate pyrophosphate decarboxylase
No full textpublished_or_final_versionDentistryDoctoralDoctor of Philosoph
The post-antifungal effect (PAFE) and its impact on the pathogenic attributes of Candida albicans
No full textpublished_or_final_versionOral BiologyDoctoralDoctor of Philosoph
In vitro studies on candida, antimycotics and oral defences
No full textpublished_or_final_versionabstracttocDentistryDoctoralDoctor of Philosoph
Predominant cultivable putative pathogens in Chinese adults with and without periodontal diseases
No full textpublished_or_final_versionDentistryDoctoralDoctor of Philosoph
The impact of the oral environment on Candida biofilm development
No full textpublished_or_final_versionabstractDentistryDoctoralDoctor of Philosoph
Hairy leukoplakia: a new clinical entity.
No full textRecent advances in therapy mean that prompt diagnosis of AIDS can prolong the life of those infected. Hairy leukoplakia is a new clinical entity that is one of the early signs of HIV seropositivity, and the GDP is well placed to recognize this lesion and refer patients on for treatment. In this second article in the twice-yearly Aids Commentary series the author describes how to diagnose this new condition, and the treatments that are available.link_to_subscribed_fulltex
Ultrastructural, histochemical and molecular features of the invasive phase of candida species
No full textpublished_or_final_versionabstractDentistryDoctoralDoctor of Philosoph
Molecular and phenotypic characteristics of the genus actinomyces withparticular reference to the human oral cavity
No full textpublished_or_final_versionabstracttocDentistryDoctoralDoctor of Philosoph
Pathogenic features of Candida parapsilosis: an emerging fungal pathogen
No full textpublished_or_final_versionOral BiologyDoctoralDoctor of Philosoph
A novel antifungal for Candida infections : its in vitro and in vivo efficacy and proposed mechanism of action
No full textCandida is the major fungal pathogen in humans that causes infections from superficial mucosal to systemic mycoses. In recent years, the incidence of Candida infections has increased due to the growing body of immunocompromised host populations. In addition, shifting of species distribution and the emergence of antifungal resistance have caused frequent treatment failure. These key issues highlight an urgent need for new antifungals.
In such a quest, this research involved a high-throughput screening of a library of 50,240 small molecules for yeast-to-hypha transition inhibitors, to identify and characterize novel small molecules with antifungal properties. From the highthroughput screening, 20 novel small molecules were identified as yeast-to-hypha inhibitors. Their antifungal and anti-biofilm properties were further examined with a wide range of Candida species. Following the extensive screening assays, SM21, which demonstrated the most potent antifungal activity, was selected as the final hit compound. Subsequently, SM21 was evaluated comprehensively for its in vitro and in vivo efficacy. In vitro, SM21 displayed strong inhibition of yeast-to-hypha transition at low concentrations and under hyphae-inducing conditions for a wide range of reference and clinical Candida strains. Moreover, SM21 exhibited anti-biofilm activity and also anti-adhesion activity of Candida on denture acrylic surfaces. With regard to the high prevalence of denture stomatitis, a new denture material incorporating SM21 was produced and evaluated. The SM21-incorporated denture material exhibited anti-adhesion activity and steadily released SM21 in a time-dependent manner. SM21 was also shown to be non-cytotoxic in vitro and in vivo. In vivo efficacy of SM21 was examined in systemic and oral candidiasis mouse models. SM21 treatment was successful in reducing mortality rate and fungal burden of the organs in the systemic candidiasis mouse model. SM21 treatment significantly reduced tongue lesions in the oral candidiasis mouse model. Lastly, the mechanism of action of SM21 was examined by transcriptomics (microarray) and proteomics approaches (label-free quantitative mass spectrometry). The expression of genes and proteins involved in the synthesis of components of the Candida cell wall and plasma membrane were significantly modulated by SM21. Hence, it was proposed that
SM21 targets both the Candida cell wall and plasma membrane. In conclusion, the newly discovered small molecule, SM21, which possesses potent in vitro and in vivo efficacy, appears to be potentially a promising agent in managing Candida infections.published_or_final_versionDentistryDoctoralDoctor of Philosoph
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