8 research outputs found
Effect of methionine and lactic acid bacteria as aflatoxin binder on broiler performance
No full textPHYSICAL AND CHEMICAL PROPERTIES OF ORGANIC MINERAL ADDITIVE FOR RUMINANT THROUGH BIOLOGICALLY INCORPORATED BY SACCHAROMYCES CEREVISIAE IN DIFERENCE SUBSTRATES
No full textThis research was conducted to evaluate physical and chemical properties of organic mineral additive incorporated by Saccharomyces cerevisiae which was cultivated on different growth media consisted of cassava flour and rice bran, respectively. Treatment was arranged on completely randomized design consisted of rice bran without inoculants (RBo), rice bran with inoculants (RBi), cassava flour without inoculants (CFo), cassava flour with inoculants (CFi). Substrates were fortified by microminerals contained of Cu (100 ppm), Mn (100 ppm), Zn (100 ppm), I (10 ppm), Fe (2.5 ppm) and Co (2 ppm). Inoculation of S. cerevisiae could reduce fungal contamination. Physical characteristic of cassava flour was better in flavour and texture than rice bran in which tends to rancidity. Nutrients composition was similar in cassava and rice bran however crude fibre content in rice bran tends to be higher after fermentation. Mineral content (Zn and Fe) relatively decreased and in substrate supplemented by inoculant and rice bran had higher than cassava. In summary, micromineral was incorporated in cassava flour with inoculated S. cerevisiae had better than rice bran.Keywords: Organic mineral, cassava flour meal, S. cerevisiae, rice bran, ruminant
In vitro gas production kinetics and digestibility of king grass (Pennisetum hybrid) added by organic mineral and natural crude tannin
No full textThe Use of R Programming Language for Modelling
No full textThe in vitro gas production technique is one of the feed evaluation methods that capture the characteristics of rumen microbial fermentation in response to the gas produced by feed ingredients. The gas production response is related and modelled to a unit of time with several parameters using non-linear regression mathematical equations. To facilitate this task, a script program in R has been developed that can perform the intended calculations. The calculation process uses nonlinear mathematical equations with intercepts. The equation shows that gas production (P) (mL) is influenced by several constants that indicate the share of gas production from the soluble fraction (FA) (mL), gas production from the potentially soluble part (FB) (mL), gas production rate (KC) (mL/h) and incubation time (T) (h). In addition to P and T, the three constants are tried to be derived by the program created. The data set is taken from several in vitro gas production studies that have been conducted previously. The Neway Software program of curve fitting calculations was used to validate the results, as the mean difference was tested using an unpaired T-test. Both programs showed the same calculation results for FA (P=0.9996), FB (P=0.9998), and KC (P=0.9962). The residual standard error (RSE) and R-square (R2) values of both programs also came with the same result they were RSE (P =0.9978), and R2 (P=0.6087) respectively. This shows that the R program script made can carry out the calculation process of making a model of the gas production equation for modeling in vitro gas production from rumen fermentation
Effects of slow-release ammonium sulfate as non-protein nitrogen sources on rumen fermentation characteristics: An in vitro study
No full textIn vitro anthelmintic activity of Chaetomorpha vieillardii ethanolic extract against adult worm motility and egg-hatching of Haemonchus contortus from sheep
No full textThis study aimed to evaluate the potential of macroalgae Chaetomorpha vieillardii as an in vitro candidate for bio-anthelmintic. Adult Worm Motility Test (AWMT) was conducted at concentration levels of 2 mg/mL, 4 mg/mL, and 6 mg/mL of C. vieillardii ethanol extract on 150 female H. contortus worms, randomly divided into 6 treatments with 5 replications, using a two way completely randomized factorial design. 0.9% sodium chloride was used as a negative control, and albendazole at 0.5 and 2 mg/mL served as positive controls. Worm motility observations were performed at 0, 0.25, 0.5, 1, 2, and subsequent time intervals until 100% of the worms were deceased. The identical treatment was applied to the egg hatching inhibiting test (EHIT) using a completely randomized design in a one-way pattern by observing the number of eggs at 0 and 24 hours. The results showed that the concentration level of C. vieillardii ethanol extract and the observation time significantly influenced (P<0.01) the motility of H. contortus, and there was an interaction between them (P<0.01). All three levels were able to decrease the motility of H. contortus, but they were unable to match albendazole. Despite of this result, the three levels were equally effective (P<0.01) as albendazole in terms of inhibiting the egg-hatching of H. contortus. The 2 mg/mL level was sufficient to inhibit the motility and egg-hatching of H. contortus. These findings indicated that the ethanol extract of C. vieillardii has the potential to be developed as a bio-anthelmintic for ruminants
Effects of various macroalgae species on methane production, rumen fermentation, and ruminant production: A meta-analysis from in vitro and in vivo experiments
No full textA meta-analysis was performed to examine the dietary inclusion of marine macroalgae species to target methane (CH4) reduction from ruminant animals and the effects on rumen fermentation and animal performance. A literature search was conducted from global scientific databases, resulting in 25 in vitro and 22 in vivo studies eligible to be integrated in a database. A total of 673 experimental units comprising 537 in vitro and 136 in vivo experimental units were analyzed by using mixed model methodology in SAS and multivariate analysis in R Studio. Principal component analysis (PCA) from in vitro dataset revealed difference effects of brown, green, and red macroalgae on CH4 production without a pronounced pattern on rumen fermentation. Likewise, PCA from in vivo dataset supported the in vitro results whereas Ascophyllum nodosum (brown) and Asparagopsis taxiformis (A. taxiformis; red) species showed noticeably separated clusters on CH4 production. Mixed regression analysis from in vitro and in vivo databases showed interaction effects (P 0.05) compared with control diet as a reference. Curvilinear effect on in vitro organic matter digestibility (P = 0.043) and linear degreased on crude protein digestibility (P = 0.029) were found. Feeding macroalgae did not affect dry matter intake (DMI), average daily gain (ADG), milk production and milk composition except for milk lactose which linearly increased (R2 = 0.731; P = 0.002) by increasing Ascophyllum nodosum level. In dairy cows, the inclusion of A. taxiformis increased iodine concentration by more than six-fold increase (P 10 g/kg DM of diet may result in an unfavorable effect on the high bromoform and iodine residuals in milk. Future in vivo study using less-explored species that had been tested in vitro need to be conducted
