94 research outputs found
Pyridoxamine driven selective turn-off detection of picric acid using glutathione stabilized fluorescent copper nanoclusters and its applications with chemically modified cellulose strips
No full textThe present work reports the interaction of various vitamin B6cofactors with the red emitting glutathione stabilized copper nanoclusters (GSH-CuNCs). Addition of pyridoxamine (PM) resulted a new turn-on band at 410 nm due to the possible adsorption over the surface of GSH-CuNCs. The nano-assembly PM-GSH-CuNCs was applied for the selective detection of nitro-aromatic compounds. Upon addition of picric acid (PA), the fluorescence of PM-GSH-CuNCs was selectively quenched at 410 nm and ~ 625 nm among the other tested nitro-aromatic compounds. With a linearity range from 9.9 Î1⁄4M to 43 Î1⁄4M, the concentration of PA can be detected down to 2.74 Î1⁄4M. The high selectivity exhibited by the nano-assembly allows to detect PA in real samples like tap water, river water and matchstick. Advantageously, the nano-assembly PM-GSH-CuNCs was chemically adsorbed over the cellulosic strips and applied for the naked-eye detection of PA down to 1 Î1⁄4M
Three-in-one type fluorescent sensor based on a pyrene pyridoxal cascade for the selective detection of Zn(ii), hydrogen phosphate and cysteine
No full textA novel fluorescent receptor L was synthesized by Schiff base condensation of 1-pyrenemethylamine with the vitamin B6 cofactor pyridoxal. The receptor L is highly selective and sensitive towards Zn2+ ions among other tested metal ions. Upon interaction with Zn2+, the receptor L showed a distinct fluorescence enhancement at 485 nm due to the excimer formation leading to the fluorescent colour change from blue to bluish-green. Subsequently, when the in situ generated ZnL2 complex interacted with various anions and amino acids, the addition of H2PO4- and cysteine reinstated the fluorescence of the receptor L due to the demetalation of Zn2+ from the ZnL2 complex. Accordingly, the receptor L was developed for the highly selective, specific and sensitive detection of three important bioactive analytes, i.e., Zn2+, H2PO4- and cysteine with a detection limit down to 2.3 × 10-6 M, 2.18 × 10-7 M and 1.59 × 10-7 M, respectively. Additionally, the receptor L was applied to the detection of intracellular Zn2+ ions in live HeLa cells
SLOVENIAN GOVERNMENTAL STAKEHOLDERS IN THE FIELD OF MARITIME SURVEILLANCE
No full textDelo predstavi vladne deležnike na področju pomorstva v Republiki Sloveniji, vključujoč njihove naloge, način delovanja in sestav različnih oddelkov. Ker deležniki za potrebe opravljanja nalog zbirajo informacije s svojega področja delovanja, bodo v nadaljevanju predstavljeni tudi t. i. senzorji za zbiranje podatkov. Opisani bodo vsi načini deljenja podatkov s splošno javnostjo in ostalimi službami z namenom skupnega reševanja problemov in obravnave dogodkov. V zaključku dela bodo na podlagi zbranih informacij o delovanju deležnikov identificirani pomanjkljivosti in predlogi za njihovo izboljšanje.The author shall define and present all Slovenian governmental stakeholders in the area of maritime surveillance including their tasks, modus operandi and departmental structure. To perform their tasks efficiently, the stakeholders gather various data with the help of so-called sensors, thus an introduction to those shall follow. Furthermore, ways of private and public data sharing for the sake of inter departmental problem solving and discussion shall be described. Based on gathered information, the author will form a conclusion by identifying deficiencies in the stakeholder’s work and shall provide suggestions for their improvement
A short overview on fluorescent nanoclusters and its application in sensing of metal ions
No full textDepartment of Applied Chemistry, S. V. National Institute of Technology (SVNIT), Surat-395 007,
Gujarat, India
E-mail : [email protected], [email protected]
Manuscript received 30 November 2017, accepted 01 December 2017
The fluorescence of noble metal nanoclusters (NCs) has attracted burgeoning interest among the chemists working in the field of sensors and biosensors development due to their unique properties such as excellent photostability, low toxicity, bio-compatibility and size-dependent fluorescence. The size of noble metal NCs is between metal atoms and nanoparticles, and comparable to the Fermi wavelength of electrons, which resulting in the molecule-like properties and discrete energy levels to show size-dependent fluorescence. Because of the excellent fluorogenic properties, the noble metal NCs are widely used for the development of fluorescent probes for various applications. This short review was narrated to summarize some selected applications of noble metal NCs (mainly of gold and silver) in the detection of metal ions
Recent Advances on Iron(III) Selective Fluorescent Probes with Possible Applications in Bioimaging
No full textIron(III) is well-known to play a vital role in a variety of metabolic processes in almost all living systems, including the human body. However, the excess or deficiency of Fe3+ from the normal permissible limit can cause serious health problems. Therefore, novel analytical methods are developed for the simple, direct, and cost-effective monitoring of Fe3+ concentration in various environmental and biological samples. Because of the high selectivity and sensitivity, fast response time, and simplicity, the fluorescent-based molecular probes have been developed extensively in the past few decades to detect Fe3+. This review was narrated to summarize the Fe3+-selective fluorescent probes that show fluorescence enhancement (turn-on) and ratiometric response. The Fe3+ sensing ability, mechanisms along with the analytical novelties of recently reported 77 fluorescent probes are discussed
Pyridoxal conjugated gold nanoparticles for distinct colorimetric detection of chromium(<scp>iii</scp>) and iodide ions in biological and environmental fluids
No full textWe developed pyridoxal conjugated gold nanoparticles (CAPy-AuNPs) for the selective colorimetric detection of Cr3+ and iodide ions in an aqueous medium.</p
Study of Anticancer Drugs Interaction with Hemoglobin by Electrochemical Methods and Molecular Docking: Implications towards Anticancer Treatment
No full textDeveloping a Cost-Effective Bioassay to Detect Alkaline Phosphatase Activity and Generating White Light Emission from a Single Nano-Assembly by Conjugating Vitamin B<sub>6</sub> Cofactors with Lysozyme-Stabilized Fluorescent Gold Nanoclusters
No full textIn
this work, lysozyme-stabilized fluorescent gold nanoclusters
(Lyso-AuNCs) and pyridoxal 5′-phosphate (PLP) as a monophosphate
ester substrate were used to develop a highly selective and cost-effective
bioassay for the detection of alkaline phosphatase (ALP) activity.
The vitamin B6 cofactor, PLP, was conjugated with the red-emitting
nanoclusters to obtain a probe, PLP_Lyso-AuNCs, via forming a Schiff base linkage between the free amino group of lysozyme
and the aldehyde group of PLP. At pH = 10.08, addition of ALP to the
yellow-emitting PLP_Lyso-AuNCs solution catalyzed the hydrolysis of
PLP and converted it into pyridoxal, which produced a distinct ratiometric
fluorescence response and the fluorescent color turned pale white.
Using the probe, PLP_Lyso-AuNCs, the ALP activity could be detected
down to 0.002 U/L. Further, the changes in the fluorescent color intensity
(red, blue, and green) of PLP_Lyso-AuNCs were recorded with the back
camera of a smartphone to quantify the ALP activity. Both the fluorimetric
and smartphone approaches gave satisfactory recovery percentage, when
the practical utility of PLP_Lyso-AuNCs was applied to quantify the
ALP activity in environmental and biological samples, such as river
and lab tap water, blood plasma, and serum. Finally, a pure white
light-emitting nano-assembly was developed by conjugating optimized
amounts of both PLP and pyridoxal over the surface of Lyso-AuNCs
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