2,087 research outputs found

    MODEL PENGEMBANGAN PENDIDIKAN KARAKTER BERBASIS PENGUATAN BUDAYA SEKOLAH RELIGIUS DI SMA ISLAM SULTAN AGUNG 3 SEMARANG

    No full text
    The problem of national identity and character lately is at risk. The number of negative events and behaviors, both individually and in groups in the community, illustrates the degadration of the nation's moral values. The character crisis is characterized by an increasing number in crime and violence in the society, including in the world of education. This fact encourages the emergence of various lawsuits on the effectiveness of character education in schools which so far have been seen by some communities as having failed in building students' affection with eternal values and being able to answer the challenges of the changing times.That the crisis of character, one of which is caused by a lack of understanding and practice of religious teachings. Religious characters that require students to understand and be able to practice the teachings of their religion become one of the most important character points in order to improve the good character of students. Religious becomes the foundation of the nation's character, so we need a way to build religious character for each student. Departing from this, in this article the author will discuss about the development model of religious school culture-based character education. The problem in this article will be focused first, on how to develop character education models based on the strengthening of religious school culture in Sultan Agung 3 Islamic High School Semarang; second, how the results of the implementation of the development of character education based on strengthening the culture of religious schools in Sultan Agung 3 Islamic High School Semarang. This article is the result of a qualitative descriptive study conducted by the author at Sultan Agung 3 Islamic Senior High School Semarang. From the research conducted by the author, the results of this research are: the model of character education development based on strengthening the religious school culture is needed in order to increase the effectiveness of character education in schools. Keywords: Character education, character crisis, religious school culture.Â

    Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

    No full text
    Myeloperoxidase (MPO) is involved in acute and chronic inflammatory diseases. The source of MPO in acute liver diseases is still a matter of debate. Therefore, we analysed MPO-gene expression on sections from normal and acutely damaged [carbon tetrachloride-(CCl4) or whole liver gamma-Irradiation] rat liver by immunohistochemistry, real time PCR and Western blot analysis of total RNA and protein. Also total RNA and protein from isolated Kupffer cells, hepatic stellate cells, Hepatocytes, endothelial cells and neutrophil granulocytes (NG) was analysed by real time PCR and Western blot, respectively. Sections of acutely injured human liver were prepared for MPO and CD68 immunofluorescence double staining. In normal rat liver MPO was detected immunohistochemically and by immunofluorescence double staining only in single NG. No MPO was detected in isolated parenchymal and non-parenchymal cell populations of the normal rat liver. In acutely damaged rat liver mRNA of MPO increased 2.8-fold at 24 h after administration of CCl4 and 3.3-fold at 3 h after gamma-Irradiation and MPO was detected by immunofluorescence double staining only in elastase (NE) positive NGs but not in macrophages (ED1 or CD68 positive cells). Our results demonstrate that, increased expression of MPO in damaged rat and human liver is due to recruited elastase positive NGs

    Ferritin L and ferritin H are differentially located within hepatic and extra hepatic organs under physiological and acute phase conditions

    No full text
    Ferritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions. Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time points. Immunohistochemistry was performed utilizing horse-reddish-peroxidise conjugated secondary antibody on 4 mu m thick section. Liver cytoplasmic and nuclear protein were used for Western blot analysis. By means of immunohistology, FTL was detected in cytoplasm while a strong nuclear positivity for FTH was evident in the liver. Similarly, in heart, spleen and brain FTL was detected mainly in the cytoplasm while FTH demonstrated intense nuclear and a weak cytoplasmic expression. Western blot analysis of cytoplasmic and nuclear fractions from liver, heart, spleen and brain further confirmed mainly cytoplasmic expression of FTL in contrast to the nuclear and cytoplasmic expression of FTH. The data presented demonstrate the differential localization of FTL and FTH within hepatic and extra hepatic organs being FTL predominantly in the cytoplasm while FTH predominantly in nucleus

    Ferritin L and ferritin H are differentially located within hepatic and extra hepatic organs under physiological and acute phase conditions

    No full text
    Ferritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions. Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time points. Immunohistochemistry was performed utilizing horse-reddish-peroxidise conjugated secondary antibody on 4 mu m thick section. Liver cytoplasmic and nuclear protein were used for Western blot analysis. By means of immunohistology, FTL was detected in cytoplasm while a strong nuclear positivity for FTH was evident in the liver. Similarly, in heart, spleen and brain FTL was detected mainly in the cytoplasm while FTH demonstrated intense nuclear and a weak cytoplasmic expression. Western blot analysis of cytoplasmic and nuclear fractions from liver, heart, spleen and brain further confirmed mainly cytoplasmic expression of FTL in contrast to the nuclear and cytoplasmic expression of FTH. The data presented demonstrate the differential localization of FTL and FTH within hepatic and extra hepatic organs being FTL predominantly in the cytoplasm while FTH predominantly in nucleus
    corecore