1,721,009 research outputs found
Steroid receptors in breast carcinoma: comparison between the results obtained in frozen and paraffin embedded tissues.
No full textHER-2 status discrepancy between primary breast cancer and metastatic sites. Impact on target therapy.
No full textIn this prospective study, we determined HER-2 status in primary
breast invasive carcinomas and in the paired lymph node metastases
(synchronous and metachronous), local recurrence and metachronous
distant metastases, to verify the percentage of discordant
cases. HercepTestTM and Fluorescence in situ hybridization
(FISH) were used to determine HER-2 status on 119 cases of primary
infiltrating breast carcinoma and paired metastases (45
cases with synchronous lymph node metastases, 9 cases with metachronous
lymph node metastases, 30 cases with local recurrence,
and 35 cases with metachronous distant metastases). A therapeutically
significant HER-2 status discordance was demonstrated
between primary carcinoma and synchronous lymph node metastases
(6.7%), local recurrence (13.3%) and metachronous distant
metastases (28.6%). In the first comparison, there was a normal
HER-2 status in primary tumours and HER-2 amplification in
paired metastases, in the second the opposite phenomenon was
present, and both types of discordance were evident in the third
comparison. Considering the cases of local recurrences and metachronous
distant metastases all together, 14 out of 65 cases
(21.5%) showed a therapeutically significant discordance of HER-
2 status between the primary tumour and the paired metachronous
recurrence or metastasis (p < 0.001), the 15.4% of cases
showing normal HER-2 status in the primary tumour and HER-2
amplification in the neoplastic relapse. For the treatment of metastatic
patients, the evaluation of HER-2 status should be performed
in neoplastic tissue from metastatic site, whenever possible.
This procedure could be also suggested in the patients that are
metastatic at the time of diagnosis
Glycodelin and p16 expression in cervical intraepithelial neoplastic lesions: differences between pregnant and non-pregnant patients
Full text linkAnalysis of immunohistochemical expression of inducible nitric oxide synthase for the evaluation of agonal time in forensic medicine
No full textAlthough establishing agony is crucial in forensic practice, the identification of specific signs indicative of a detailed duration of agony is however not of immediate execution. Nitric oxide (NO) is the most important messenger molecule in the modulation of vascular tone and it is produced during stress conditions by inducible nitric oxide synthase (iNOS), as occurs during agony. The aim of this study was to investigate the relationship between immunohistochemical expression of iNOS, and agonal time (T), defined as the interval between the onset of a hypoxic-ischemic injury and the death. INOS expression was evaluated by measuring the average of signal intensity (SI) from cytoplasm of 300 smooth muscle cells of sample of renal artery, performed by ImageJ software: high values of SI correspond to a low enzyme expression and vice versa. We aimed also to check if gender, age, type of death (violent or natural death), post mortem interval, and storage in cold chamber influenced SI. We assessed 50 autopsied cases, of which 28 violent and 22 natural deaths, with a well-known T in a range between 1 and 631 min. Statistical analysis was performed to estimate the relationship between SI and the other variables. Results pointed out that only SI is related to T, and since data showed a bi-phase relationship between T and SI, we used a piecewise regression method for estimation of T as function of SI. The transition from the first to the second phase takes place at SI = 117.5 which corresponds to a T of 29.5 min. In conclusion, the study demonstrates that iNOS is a good marker for estimating T and the final regression model can be used in many forensic activities
Terminal complement complex in synovial tissue from patients affected by rheumatoid arthritis, osteoarthritis and acute joint trauma
No full textThe C5b-9 complex (Terminal Complement Complex-TCC) is the final product of the terminal complement pathway. In this study, using the monoclonal antibody MCaE11 (specific for a C9 neoantigen) and an immunohistochemical technique, we examined the TCC deposits in synovial tissues from 4 patients affected by rheumatoid arthritis (RA) and 6 patients affected by osteoarthritis (OA). Synovial tissues from 8 patients affected by acute joint trauma were examined as controls. Furthermore, plasma TCC levels were measured in 44 RA patients and 51 controls, using the above mentioned antibody and a sandwich ELISA. Eight synovial fluids were also included in this study. Abundant TCC deposits were detected in the cytoplasm of the synovial lining cells and of large stromal mononuclear cells in all the RA and in 3 out of 6 OA synovial tissues characterized by histological signs of inflammation. No TCC deposits were found in non-inflamed synovial tissues from patients with joint trauma. In agreement with previous observations, the TCC plasma levels found were significantly higher in RA patients than in controls, but no difference was seen between patients with active and non-active disease. The mean TCC level was significantly higher in the synovial fluid than in the plasma, but no correlation emerged between these two series of values. This study shows that: a) the plasma level of TCCs cannot serve as an indicator of disease activity in RA; b) the TCC deposits in synovial tissue correlate well with the extent of inflammatory synovitis, irrespective of whether the synovitis is rheumatoid or osteoarthritic in nature
Density of neoplastic lymphoid infiltrate, CD8+ T cells, and CD1a+ dendritic cells in mycosis fungoides.
No full textExpression of pi-class glutathione S-transferase: two populations of high grade prostatic intraepithelial neoplasia with different relations to carcinoma
No full textVideoendoscopic assisted curettage of central giant cell granuloma of the maxilla in pediatric age.
No full text
Different binding to squamous and columnar epithelium of the uterine cervix as a marker of epithelial differentiation.
No full textBiotinylated lectins were used to investigate the expression of carbohydrate residues on columnar and squamous epithelium of the uterine cervix. Con A, WGA, RCA I, PNA, UEA I, DBA and SBA were used. In the native exocervical and in metaplastic squamous epithelium of the transformation zone, one group of lectins (Con A, WGA, RCA I and PNA) stained the cell periphery of all epithelial layers. A second group (UEA I, DBA and SBA) colored the cell periphery of the suprabasal cells. The basal layer was always negative. All lectins labeled the apical border and occasionally the cytoplasm of the endocervical columnar epithelium. Lectin-binding of metaplastic and native squamous epithelium could possibly be used as a marker of epithelial differentiation in normal and abnormal conditions
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