1,721,107 research outputs found
Influence of dietary starch and of phase of lactation on haematological markers of oxidative stress in early lactation
No full text
Superovulation of Marchigiana beef cattle by two administrations of porcine FSH dissolved in hyaluronan
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Nutritional regulation of mammary cell apoptosis in lactating ewes.
No full textLo stress ossidativo può essere mediato dall’apporto nutritivo, alterando il rapporto
tra la sopravvivenza e la morte cellulare per apoptosi. Alcuni componenti della dieta (grasso, amido) possono
infatti portare ad un incremento di specie reattive dell’ossigeno (ROS), che determinano un danno
cellulare ed una modificazione dell’apoptosi, con conseguenze negative sulla lattazione. Nel presente lavoro
si sono voluti studiare gli effetti di due diverse diete ad alto tenore di amido e di grasso sul bilancio cellulare
e sull’espressione di antiossidanti (GST) nelle cellule della ghiandola mammaria di pecore a 50
giorni di lattazione. L’apporto di grasso ha determinato un aumento dell’espressione di GST ed una diminuzione
della percentuale di cellule apoptotiche (1,51 unità GST e 0,72% apoptosi) rispetto al controllo
(1,04 unità e 1,75%), mentre la dieta ricca di amido ha causato un aumento dell’espressione di GST cellulare
mammario unitamente ad una diminuzione di apoptosi (0,89 unità e 0,78%)
Glycoconjugates in small antral ovarian follicles of the river buffalo (Bubalus bubalis L.)
No full textTypes and distribution patterns of glycoconjugates in antral ovarian follicles were investigated in the buffalo, using periodic-acid Schiff (PAS), high iron diamine (HID), low ion diamine (LID) and lectin histochemical staining methods. HID and LID staining procedures were preceded in some cases by digestion with testicular hyaluronidase, Streptomyces hyaluronidase, chondroitinase ABC and heparitinase (heparinase III). Lectin staining was performed with the use of 12 horseradish peroxidase (HRP) lectin conjugates. Some lectin staining procedures were preceded by neuraminidase digestion and saponification. Large amounts of isomeric chondroitin sulphates and a minor quantity of heparan sulphate and hyaluronic acid and/or chondroitin were found in follicular fluid. Lectin staining of buffalo follicular fluid revealed glycoconjugates with different glucidic determinants such as beta-N-acetylgalactosamine, beta-galactose-(1-3)-N-acetylgalactosamine, beta-galactose(1-4)-N-acetylglucosamine, N-acetylglucosamine, alpha-fucose and alpha-glucose/alpha-mannose, and sialic acid residues. Glycosaminoglycans were absent in the zona pellucida of oocytes in small antral follicles. Acidic glycoconjugates in the zona pellucida were caused by sulphated groups and sialic acid residues. Our data show few internal glucidic residues, such as N-acetylglucosamine in the buffalo zona pellucida but many subterminal beta-N-acetylgalactosamine, alpha- and beta-galactose determinants masked by sialic acids. These findings demonstrate that buffalo follicular fluid has a very heterogeneous composition that is similar to that found in small and large bovine follicles. No differences in composition of the follicular fluid were observed in the follicles examined
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