2,977 research outputs found
Analysis of Cultured Human Melanocytes Based on Polymorphisms within the SLC45A2/MATP, SLC24A5/NCKX5, and OCA2/P Loci.
No full textReflections of a Jewish, Lesbian Author
No full textIn this essay, Jewish lesbian author Leslea Newman speaks of the importance of finding one's own identity reflected in works of literature, citing examples of her own work, and recommending the writings of other Jewish lesbian authors of merit
The Core Protein of the Basement-Membrane Heparan-Sulfate Proteoglycan (Bmhspg) Is Secreted Basally From Mdck Cells Treated with B-D-Xyloside
No full textDistinct Coated Vesicles Labeled for P200 Bud From Trans-Golgi Network Membranes
No full textGolgi-associated cytoplasmic proteins, such as the coatomer protein complex, are required for vesicle budding and trafficking. We have previously described a cytoplasmic phosphoprotein, p200, which binds dynamically and specifically to Golgi membranes. The p200 protein is dissociated from Golgi membranes in the presence of brefeldin A and it is induced to bind to Golgi membranes by activation of guanine nucleotide binding proteins (G proteins) with guanosine 5'-[gamma-thio] triphosphate or aluminum fluoride. To establish the role of p200 in vesicle budding, we localized membrane-bound p200 in intact cells and on isolated Golgi membranes. We show that p200 is preferentially associated with vesicles in the trans-Golgi network (TGN). Activation of G proteins induced budding and accumulation of small, coated vesicles from Golgi membranes and p200 was localized on the cytoplasmic surface of some of these resides. Using immunogold labeling we further demonstrate that p200 and beta-COP are localized on different populations of Golgi-derived vesicles. These data establish that p200 is involved in the budding and coating of a class of Golgi vesicles that are likely to be derived from the TGN. The data also show that there are distinct populations of non-clathrin-coated vesicles budded from Golgi membranes, and vesicles labeled for either beta-COP or p200 may represent transport vesicles for separate steps of protein transport
Moving Things Around - the Structural Basis of Intracellular Protein Trafficking - November 17-20, 1993 Chatham-Bars-Inn, Chatham, Ma
No full textProtein trafficking and polarity in kidney epithelium: From cell biology to physiology
No full textThe transepithelial movement of fluids, electrolytes, and larger molecules is achieved by the activity of a host of specialized transporting proteins, including enzymes, receptors, and channels, that are located on either the apical, basal, or lateral plasma membrane domains of epithelial cells. In the kidney as well as in all other organs, this remarkable polarity of epithelial cells depends on the selective insertion of newly synthesized and recycling proteins and lipids into distinct plasma membrane domains and on the maintenance and modulation of these specialized domains once they are established during epithelial development, This review addresses the mechanisms by which epithelial cells control the movement of membrane components within the cell to ensure that they are delivered to the correct target membrane. Among the topics discussed are targeting signals within membrane proteins, the role of the cytoskeleton and the tight junctional barrier in cell polarity, and the requirement for accessory proteins in the targeting process, including GTP-binding proteins, and proteins that are involved in vesicle docking and fusion events. The final part of the review is devoted uniquely to the polarized targeting of functionally defined proteins in various kidney cell types. In concluding, examples of how a breakdown in these trafficking pathways may be related to some disease states are presented
Staging the life-world: Habermas and the recuperation of Austin speech act theory
No full textPT: J; CR: APEL KO, 1976, SPRACHPRAGMATIK PHIL AUSTIN JL, 1962, HOW TO DO THINGS WOR AUSTIN JL, 1970, PHILOS PAPERS CULLER J, 1982, DECONSTRUCTION DERRIDA J, 1977, GLYPH, V1 ECO U, 1992, UNDERSTANDING ORIGIN, P273 FISH S, 1987, TRACING LIT THEORY HABERMAS J, 1984, THEORY COMMUNICATIVE, V1 HABERMAS J, 1987, THEORY COMMUNICATIVE, V2 HABERMAS J, 1989, JURGEN HABERMAS SOC MARTINET A, 1962, FUNCTIONAL VIEW LANG, P24 QUINE WV, 1960, WORD OBJECT SEARLE JR, 1969, SPEECH ACTS SEARLE JR, 1977, GLYPH, V1 VANEEMEREN F, 1983, SPEECH ACTS ARGUMENT WARNOCK GJ, 1989, FL AUSTIN; NR: 16; TC: 0; J9: J THEOR SOC BEHAV; PG: 12; GA: KR147Source type: Electronic(1
MODEIN2 and Colby: computer codes for sediment transport computations
No full textNovember, 1976.CER76-77VMP-JL-DBS19
Tunneling microscopy of NbSe2 in air
No full textPT: J; CR: BANDO H, 1987, JPN J APPL PHYS PT 2, V26, L41 BINNIG G, 1982, PHYS REV LETT, V49, P57 BRYANT A, 1986, APPL PHYS LETT, V48, P832 FELDMAN JL, 1976, J PHYS CHEM SOLIDS, V37, P1141 FELDMAN JL, 1981, J PHYS CHEM SOLIDS, V42, P1029 JERICHO MH, 1980, PHYS REV B, V22, P4907 JERICHO MH, 1987, REV SCI INSTRUM, V58, P1349 MAMIN HJ, 1986, PHYS REV B, V34, P9015 PETHICA JB, 1986, IBM J RES DEV, V30, P455 RAO GVS, 1979, PHYSICS CHEM MATERIA, P99 SOLER JM, 1986, PHYS REV LETT, V57, P444 TERSOFF J, 1986, PHYS REV LETT, V57, P440 TOKUMOTO H, 1986, JPN J APPL PHYS, V25, L621; NR: 13; TC: 14; J9: J APPL PHYS; PG: 4; GA: L5219Source type: Electronic(1
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