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Biochemical and protein profile of alpaca (Vicugna pacos) uterine horn fluid during early pregnancy
Full text linkSouth American camelids show high embryo loss rate, during the first 60 days of pregnancy. One of the factors which may be related to this situation is that over 98% of the embryos implant in the left uterine horn (LUH) even though both ovaries contribute similarly to ovulation. There is scarce information about the uterine environment of female camelids at any physiological state that could explain the capability of the LUH to attract the embryo and maintain pregnancy. We describe, for the first time, the biochemical and protein profile of uterine fluid (UF), addressing the right and LUH environment in non-pregnant and pregnant alpacas. Different substrates, electrolytes and metabolites were assayed in both uterine horn fluids. Small changes were observed in glucose and total protein levels, which were more noticeable during pregnancy. In addition, 10 specific proteins were found in the left horn fluid in 5-week-pregnant alpacas, and two protein bands were identified in non-pregnant alpaca right horn fluid. These results would provide basic information for identification of possible markers for pregnancy diagnosis, reproductive diseases and hormone-treated animals evaluation and hence contributing to improve the pregnancy rate.Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Cátedra de Zootecnia General I; ArgentinaFil: Zampini, Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vencato, Juri. Università di Padova; ItaliaFil: Stelletta, Calogero. Università di Padova; Itali
Prove in vitro di adesione degli spermatozoi e il loro valore per stimare la fertilità negli animali da reddito
No full textThe classic procedure for evaluating the fertility from sires is to determine pregnancy data following artificial insemina- tion. This procedure is time consuming and expensive be- cause of the large number of young bulls entering the bree- ding program. Several studies have been done to find a sim- ple and reliable test for predicting fertility. The perfect test should take into account each and all sperm traits, determi- ning quantity and quality of fertile sperm in a single sperm sample. This review analyze the correlation between in vitro test and in vivo fertility with particular consideration to two tests used in vitro: the sperm zone pellucida binding test and the sperm oviductal cells adhesion test. The first results very useful for the evaluation of sperm destined to in vitro em- bryo production, but fails in predicting male fertility when mating or artificial insemination practices are used. The di- rect encounter between sperm and oocyte, without consider the natural physiological barriers that sperm must overpass into the female tract could be the main reason of failing. On the other hand, the sperm adhesion to the oviduct seems to be an in vivo requisite to fertilization. The oviduct is consi- dered to select the non capacitated - live - non damaged pla- sma membrane sperm that are considered as the fertilizing population. In this way the sperm - oviduct binding test has been developed. Several factors that could influence the test results such as the origin of the tissue used and the cell ex- plant size, are discussed. The available information about the use of this test is still short, however the test has a great po- tential for the sires sub-fertility evaluation, even though its accuracy should be limited to certain type of sperm samples
Purulent pericarditis from Arcanobacterium pyogenes in a bull [Pericardite purulenta da arcanobacterium pyogenes in un toro]
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