170,390 research outputs found
[2001.13.035] Minnie Buck and Regina Staib
No full textPhotographic image. Black and white. Two women in shirtwaists and skirts with broad-brimmed hats. Identified as Minnie Buck and Regina Staib, c. 1914. Image courtesy of Ludwig and Christina Graf Buck Collection, GRHC.Photographic image. Black and white. Two women in shirtwaists and skirts with broad-brimmed hats. Identified as Minnie Buck and Regina Staib, c. 1914. Courtesy of Ludwig and Christina Graf- Buck Collection, 2001.13, GRHC
Differential chlamydospore development by the analyzed <i>Candida</i> strains in Staib liquid medium.
No full text<p><i>C. dubliniensis</i> wild type Wü284 and the <i>C. albicans nrg1</i>Δ mutant MMC3 form chlamydospores, in contrast to the <i>C. albicans</i> wild type SC5314. The fungal strains were grown for 28 h in Staib medium at 25°C and inspected by microscopy (scale bar: 10 µm).</p
Herman Staib and Anna C. Fritz 1879 Marriage License
No full textThis marriage license was issued from the Office of the Probate Court in Seneca County, Ohio to authorize the marriage of Herman Staib and Anna C. Fritz. The date on the license is March 10, 1879. The wedding date is listed as March 11, 1879, with the ceremony conducted by Pastor C. Zimmerman. The license is owned by St. John's United Church of Christ, 10 Main Street, Tiffin (Ohio
DRL6 induces chlamydospores in Staib agar.
No full text(A) Abundant chlamydospore formation by C. dubliniensis Wü284. (B) Poor filamentation and no detectable chlamydospores by C. albicans SC5314. (C) Occasionally visible chlamydospore formation by DRL6 (Δisw2/Δisw2) strain in Staib agar as indicated by the black arrow heads. Plates were grown at room temperature in the dark for up to 7 days, and representative pictures are from at least 3 independent experiments.</p
[2001.13.031] Wilhemina "Minnie" Buck, (Christine Neumann or Lydia Buck), Elizabeth Buck, and Rosella Staib
No full textPhotographic image. Black and white real photo postcard. Four women in light-colored linen dresses posed in a line. Identified as (L to R): Wilhemina Buck, Christine Neumann or Lydia Buck, Elizabeth Buck, and Rosella Staib, c. 1918. Image courtesy of Ludwig and Christina Graf Buck Collection, GRHC.Photographic image. Black and white real photo postcard. Four women in light-colored linen dresses posed in a line. Identified as (L to R): Wilhemina "Minnie" Buck, Christine Neumann or Lydia Buck, Elizabeth Buck, and Rosella Staib, c. 1918. Courtesy of Ludwig and Christina Graf- Buck Collection, 2001.13, GRHC
Identificaci\uf3n de aislados de cryptococcus neoformans usando agar staib sin creatinina
No full textEn micolog\ueda, el cultivo es la mejor manera de realizar el diagn\uf3stico definitivo de una micosis. Muchos tipos de medios han sido desarrollados y modificados para tal fin, utilizando las propiedades bioqu\uedmicas de los hongos para reconocerlos. Los medios m\ue1s comunes son Sabouraud, agar Malta, agar ma\uedz, agar Papa-dextrosa, y agar Staib. Este \ufaltimo ha sido ampliamente usado para la identificaci\uf3n de levaduras del g\ue9nero Cryptococcus y en otros hongos. El objetivo de este estudio fue demostrar la utilidad del agar Staib y de un medio de Staib modificado, sin creatinina, en el aislamiento e identificaci\uf3n de hongos pat\uf3genos. Cuarenta y seis aislados de Cryptococcus neoformans m\ue1s un control fueron identificados por criterios morfol\uf3gicos y bioqu\uedmicos, usando la prueba de ureasa (agar urea de Christensen) y cultivo en medio Sablac. Las cepas fueron transferidas a placas de agar Staib estandar y de agar Staib sin creatinina. Las placas fueron incubadas a temperatura ambiente (26-28\ub0C) durante una semana y luego fueron evaluadas las caracter\uedsticas morfol\uf3gicas de las colonias y producci\uf3n de fenoloxidasa. En 43 (91.5%) de las cepas estudiadas, se observ\uf3 producci\uf3n de fenoloxidasa (presencia de colonias marrones) tanto en el agar estandar como en el agar sin creatinina. Conclusi\uf3n: el agar Staib sin creatinina es un medio excelente para la identificaci\uf3n de C. neoformans, con las ventajas de un bajo costo y f\ue1cil preparaci\uf3n
Jacob Staib, Jr. and Alice M. Arbogast 1879 Marriage License
No full textThis marriage license was issued from the Office of the Probate Court in Seneca County, Ohio to authorize the marriage of Jacob Staib, Jr. and Alice M. Arbogast. The date on the license is October 20, 1879. The bottom portion of the license shows the wedding date as October 23, 1879, with Pastor C. Zimmerman officiating. The license is owned by St. John's United Church of Christ, 10 Main Street, Tiffin (Ohio
Joseph W. Parks and Matilda Staib 1879 Marriage License
No full textThis marriage license was issued from the Office of the Probate Court in Seneca County, Ohio to authorize the marriage of Joseph W. Parks and Matilda Staib. The date on the license is November 27, 1879. The lower portion of the license shows the wedding date as November 27, 1879, with Pastor C. Zimmerman officiating. The license is owned by St. John's United Church of Christ, 10 Main Street, Tiffin (Ohio
Global transcriptome sequencing identifies chlamydospore specific markers in Candida albicans and Candida dubliniensis
No full textArt.e61940Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens.8Nr.
Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
No full textCandida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens
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