393 research outputs found
Association between Pulse Wave Velocity and Bone Mineral Density in Women: the Baltimore Longitudinal Study of Aging.
No full textA4NT : Author Attribute Anonymity by Adversarial Training of Neural Machine Translation
No full textText-based analysis methods enable an adversary to reveal privacy relevant author attributes such as gender, age and can identify the text's author. Such methods can compromise the privacy of an anonymous author even when the author tries to remove privacy sensitive content. In this paper, we propose an automatic method, called the Adversarial Author Attribute Anonymity Neural Translation (), to combat such text-based adversaries. Unlike prior works on obfuscation, we propose a system that is fully automatic and learns to perform obfuscation entirely from the data. This allows us to easily apply the system to obfuscate different author attributes. We propose a sequence-to-sequence language model, inspired by machine translation, and an adversarial training framework to design a system which learns to transform the input text to obfuscate the author attributes without paired data. We also propose and evaluate techniques to impose constraints on our model to preserve the semantics of the input text. learns to make minimal changes to the input to successfully fool author attribute classifiers, while preserving the meaning of the input text. Our experiments on two datasets and three settings show that the proposed method is effective in fooling the attribute classifiers and thus improves the anonymity of authors
Fresh-pack potatoes: handling, packaging and transportation in refrigerated railcars
No full textBulletin no. 804 Moscow, Idaho :University of Idaho, College of Agriculture, Agriculture Experiment Station, 1998-07-01. Author(s): Shetty, Kiran; Casada, Mark; Zhu, Hua; Thornton, Mike; Nolte, Phili
Arterial stiffness and bone demineralization: the Baltimore longitudinal study ofaging
No full textBACKGROUND: Arterial stiffening is one of the hallmarks of vascular aging, and is
an important risk factor for cardiovascular morbidity and mortality. Aging is
also associated with bone demineralization. Accumulating evidence indicate that
arterial stiffness and bone demineralization might share common pathways. The
aims of this study were to evaluate whether the association between arterial
stiffness and bone demineralization is independent of age, and to explore
putative mechanisms that may mediate their relationship.
METHODS: A cross-sectional analysis was performed using data from 321 men (68 ±
12 years) and 312 women (65 ± 13 years) of the Baltimore Longitudinal Study of
Aging. Arterial stiffness was assessed by carotid-femoral pulse wave velocity
(PWV) and cross-sectional cortical bone area (cCSA) was assessed at the level of
the mid-tibia with computed tomography (CT) imaging.
RESULTS: Age was significantly correlated with PWV in men (r = 0.38, P < 0.0001)
and women (r = 0.35, P < 0.0001). Age was associated with cCSA in women (r =
-0.14, P = 0.0008), but not in men. Age-adjusted linear regression analysis
showed a significant inverse association between PWV and cCSA, in women but not
in men. The association between PWV and cCSA remained significant in women after
adjusting for age, mean arterial pressure (MAP), obesity, menopause, drugs,
alcohol intake, physical activity, renal function, serum calcium, and total
estradiol concentration.
CONCLUSIONS: Independent of age and other shared risk factors, arterial stiffness
is inversely related to cortical bone area in women. The sex-specific signaling
and molecular pathways that putatively underlie the cross-talk between central
arteries and bone are not completely understood
PORTUGALIAE ELECTROCHIMICA ACTA Corrosion Inhibition of 304 SS in Hydrochloric Acid Solution by N -Furfuryl N ' -Phenyl Thiourea
No full textAbstract The inhibition of corrosion of 304 SS in 2 N HCl solutions by N -furfuryl -N ' phenyl thiourea (FPTU) has been investigated using potentiodynamic polarization techniques. The results obtained reveal that FPTU performs excellently as anodic inhibitor (IE> 93%) for 304 SS in HCl solution. The inhibitor functions through adsorption following Temkin's adsorption isotherm, and the inhibition was governed by physisorption mechanism. The thermodynamic parameters deduced for the adsorption process reveal spontaneous adsorption of the compound on the alloy steel surface
Differential involvement of Ca2+/calmodulin-dependent protein kinases and mitogen-activated protein kinases in the dopamine D1/D5 receptor-mediated potentiation in hippocampal CA1 pyramidal neurons
No full textAbstractDopaminergic neurotransmission modulates and influences hippocampal CA1 synaptic plasticity, learning and long-term memory mechanisms. Investigating the mechanisms involved in the slow-onset potentiation induced by the dopamine D1/D5 receptor agonists in hippocampal CA1 region, we have reported recently that it could play a role in regulating synaptic cooperation and competition. We have also shown that a sustained activation of MEK/MAP kinase pathway was involved in the maintenance of this long-lasting potentiation (Shivarama Shetty, Gopinadhan, & Sajikumar, 2016). However, the molecular aspects of the induction of dopaminergic slow-onset potentiation are not known. Here, we investigated the involvement of MEK/MAPK pathway and Ca2+-calmodulin-dependent protein kinases (CaMKII and CaMKIV) in the induction and maintenance phases of the D1/D5 receptor-mediated slow-onset potentiation. We report differential involvement of these kinases in a dose-dependent manner wherein at weaker levels of dopaminergic activation, both CaMKII and MEK1/2 activation is necessary for the establishment of potentiation and with sufficiently stronger dopaminergic activation, the role of CaMKII becomes dispensable whereas MEK activation remains crucial for the long-lasting potentiation. The results are interesting in view of the involvement of the hippocampal dopaminergic system in a variety of cognitive abilities including memory formation and also in neurological diseases such as Alzheimer’s disease and Parkinson’s disease
Structure Analysis Of Plant Lectin Domains
No full textLectins are multivalent carbohydrate binding proteins that specifically recognise diverse sugar structures and mediate a variety of biological processes, such as cell-cell and host-pathogen interactions, serum glycoprotein turnover and innate immune responses. Lectins have received considerable attention in recent years on account of their properties leading to wide use in research and biomedical applications. Seeds of leguminous plants are mainly rich sources of lectins, but lectins are also found in all classes and families of organisms. Legume lectins have similar tertiary structures, but exhibit a large variety of quaternary structures. The carbohydrate binding site in them is made up of four loops, the first three of which are highly conserved in all legume lectins. The fourth loop, which is variable, is implicated in conferring specificity. Legume lectins which share the same monosaccharide specificity often exhibit markedly different oligosaccharide specificities. This thesis primarily concerns with structure solution and analysis of lectins from the legume and β-prism II fold families using X-ray crystallography. Apart from having the property of specifically and reversibly binding to carbohydrates, lectins are also interesting models to study sequence-structure relationships, especially of how minor change in the sequence may bring about major changes in oligomerization and binding.
Chapter 1 gives an overview of different structural types of plant lectins and describes in detail, their carbohydrate binding features. The details of the various experimental procedures employed during the course of this research, are explained in Chapter 2.
Chapter 3 describes the crystal structure of a β-prism II fold lectin (RVL), from Remusatia vivipara, an epiphytic plant of traditional medicinal value, and analysis of its binding properties. This lectin was established to have distinct binding properties and has nematicidal activity against a root-knot nematode with the localization site identified as the high-mannose displaying gut-lining in the nematode. The crystal structure of RVL revealed a new quaternary association of this homodimeric lectin, different from those of reported β-prism II lectins. Functional studies on RVL showed that it fails to bind to simple mannose moieties yet showed agglutination with rabbit blood cells (which have mannose moieties on the surface) and some high mannose containing glycoproteins like mucin and asialofetuin. Further, ELISA and glycan array experiments indicated that RVL has high affinity to N-glycans like trimannose pentasaccharide such as in gp120, a capsid glycoprotein of HIV virus, necessary in virus-association with the host cell. The structural basis for this N-glycan binding was revealed through structure analysis and molecular modelling, and it was demonstrated that there are two distinct binding sites per monomer, making RVL a truly multivalent lectin. Evolutionary phylogeny revealed the divergence in the β-prism II fold proteins with regards to the number of sugar-binding regions per domain, oligomerization and specificity.
Chapter 4 deals with the structural studies on a galactose-specific legume lectin (DLL-II) from Dolichos lablab, a leguminous plant. The lectin was found to be a planar tetramer in the crystal structures of the native and ligand bound forms, as expected from our solution studies and phylogenetic analysis. The protein is a heterotetramer with subunits differing only in the presence or absence of a C-terminal helical region at the core of the tetramer. Due to the static disorder in all the crystals, the central helix could be oriented in either direction. Structure analysis of DLL-II proved to be an interesting endeavour as static disorder compounded with twinning in the crystal made the data processing and structure solution a challenging process. Subsequent structure and sequence alignments led to the identification of an adenine-binding pocket in the hydrophobic core of the tetramer. Based on this, DLL-II lectin was co-crystallized with adenine and the structure revealed the presence of adenine at the predicted binding site.
Chapter 5 describes the identification and analysis of potential plant lectins/lectin-like domains in the genome of Oryza sativa, using bioinformatics approaches. This project was initiated to study the occurrence of legume-lectin like domains (a predominant dicot feature) in O. sativa, which is a monocot. Later, a large scale genome analysis for all types of lectin domains was carried out through exhaustive PSI-BLAST, profile matching by HMMer, CDD and MulPSSM. The final validation was carried out by assessing the carbohydrate binding potential of the domain by examining the sugar binding sites. The primary interest in undertaking this work was to find the occurrence of association of these domains with other domains as in protein receptor kinases, where lectin is the receptor domain. Though primarily initiated as a bioinformatics project, further structural characterization was attempted by cloning, expression and purification of some of the annotated lectin proteins using prokaryotic expression systems. The protein expression was attained in reasonable amounts for a few of the annotated legume lectin homologs, however purification is yet to be achieved as the expressed proteins are insoluble.
A part of the results described in this thesis and the other related projects that the author was involved are reported in the following publications.
1) Purification, characterization and molecular cloning of a monocot mannose-binding lectin from Remusatia vivipara with nematicidal activity Bhat GG, Shetty KN, Nagre NN, Neekhra VV, Lingaraju S, Bhat RS, Inamdar SR, Suguna K, Swamy BM. 2010. Glycoconjugate J. 27(3):309-320
2) Modification of the sugar specificity of a plant lectin: structural studies on a point mutant of Erythrina corallodendron lectin Thamotharan S, Karthikeyan T, Kulkarni KA, Shetty KN, Surolia A, Vijayan M & Suguna K. 2011. Acta Crystallographica D 67(3):218-227
3) Crystal structure of a β-prism II lectin from Remusatia vivipara Shetty KN, Bhat GG, Inamdar SR, Swamy BM, Suguna K. 2012. Glycobiology 22(1): 56-69.
4) Structure of a galactose binding lectin from Dolichos lablab
Shetty KN, Lavanyalatha V, Rao RN, SivaKumar N & Suguna K (Under review)
5) Occurrence of lectin-like domains: Oryza sativa genome analysis. Shetty KN & Suguna K. (Manuscript in preparation
Weighted K-nearest neighbor algorithm as an object localization technique using passive RFID tags
No full textTechnologies using identification by radio frequencies (RFID) are experiencing rapid development and healthcare is a major application area benefiting from it. Highly pervasive RFID enables remote identification, tracking and localization of the medical staff, patients, medications and equipment, thus increasing safety, optimizing in real-time management and providing support for new ambient-intelligent services. This thesis describes and evaluates an algorithm that enables object localization and tracking using passive RFID tags. This thesis also describes scenarios of how this technology can be used as a part of building a smart trauma resuscitation room by tracking the equipments. The main contribution of this thesis is the adaptation of the Weighted K-Nearest Neighbor Algorithm as a localization technique to track objects in a confined and crowded space by using passive RFID tags. The input parameter to the algorithm is the received signal strength indicator (RSSI), which gives a measure of back-scattered radio frequencies from passive tags. While using RFID technology special attention has to be given to the placement of antennas to get the optimum result. Therefore, we analyzed various antenna placement configurations with mean error and error consistency as the two performance parameters. The detection of multiple tags and human occlusion are two major concerns while tracking tags in a confined space with many team members collaborating on solving a problem. The RF signal can be interrupted by people walking around randomly and holding multiple (tagged) instruments at the same time. While the algorithm worked fine when tracking multiple tags, we had to modify the experimental set-up and attach an antenna onto the ceiling (which we call a vertical antenna), so that even if all the wall antennas are blocked we get at least one input parameter to base our localization decision on. We evaluated the algorithm for different combinations of configurations and number of neighbors, and achieved the following results. The best results were obtained for the 3 antennae (placed orthogonally) configuration considering the 4 nearest neighbors wherein a mean error rate of 15% of the maximum possible error was achieved under ideal conditions. We tested the algorithm for different human occlusion scenarios i.e. blocking 1 or 2 wall antennas, standing in random positions and then roaming in the field area randomly. The mean error rate for the standing scenario was measured as 20% of the maximum possible error and 18% in the case of roaming configuration. The error was found to be consistently within our defined maximum error for 100% of the recorded readings. The results obtained were found to be satisfactory for our application where, more than the exact location of the object, knowing whether the object is within a particular region is good enough for the users to know what task is being carried out in the trauma bay. Also the algorithm holds good in an indoor environment having a lot of factors and materials which affect the RF signal disrupting accurate calculation of the location co-ordinates. The algorithm does not require extensive data collection prior to implementation which makes it easily deployable in any environment. Apart from the problems mentioned there are some other factors like materials on which the tags are attached and orientation of tags which were found to be potential hindrances for accurate localization. Acceptable solutions to these problems form a part of our future work.M.S.Includes bibliographical referencesby Akshay Shett
<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Inhibition of corrosion of stainless steel (304 SS) by N-(2-mercaptophenyl)-N<sup>'</sup>-phenyl thiourea in hydrochloric acid</span>
No full text317-322The inhibition effect of N - (2-mercaptophenyl) –N'-
phenyl thiourea (MPTU) on the corrosion of 304 SS in 1 N HCl medium has been
investigated by potentiodynamic polarization, electrochemical impedance
spectroscopy and weight loss methods. The results reveal that MPTU is an
efficient mixed inhibitor with efficiency of greater than 90% at 40ºC. The
adsorption of this inhibitor on the 304 SS surface obeys Temkins adsorption
isotherm, and the inhibition is governed by physisorption mechanism. The influence of parameters like temperature and inhibitor concentration on the corrosion rate
of 304 SS has also been investigated. The thermodynamic parameters deduced for
the adsorption process reveal a strong interaction and spontaneous adsorption
of MPTU on the alloy steel surface
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