1,720,972 research outputs found
Nappa microarrays and Mass Spectrometry: new trends and challenges
No full textNanoscienceWorks.org, (Taylor & Francis Group, LLC
CHO Proteome Alterations Induced by Reverse Transformation
No full textWe present our results about the investigation of the overall proteome alterations of Chinese Hamster Ovary Cells (CHO) after reverse transformation induced by cyclic 3’,5’-monophosphate adenosine led coupling both 1D and 2 D SDS-PGE and Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) 9Mass Spectrometry. In the same time we analyzed the effectiveness and limitation of such approach, usually adopted for protein separation and identification, in the case of very complex samples as eukaryotic cells. We performed a subcellular fractionation to separate the whole cellular proteome in four fractions in order to reduce proteome complexity and to facilitate subsequent proteome analysis. A new unique pattern of protein synthesis and processing emerged after reverse transformation involving all four cellular compartments but, at the same time, it appeared clear that the approach adopted to study such a complex biological system is not enough powerful to allow the identification of all the involved proteins
Emerging Technologies In The Study Of Key-proteins For The Control Of Nuclear Structure
No full textBy the combination of emerging biophysical technologies , we present the very preliminary study addressed to the study of two major proteins involved in the control of nuclear structure, namely in this poster the H4 histone chromosomal proteins and lysozymes as model system. The aminoterminal nucleoplasmic fragment of the lamin B receptor (LBR) is in progress.Protein structure is being characterized and compared down to the atomic scale by high resolution proton-NMR and by nanocrystallography, The same proteins are also being characterized by MALDI TOF Mass Spectrometry
NAPPA based nanogravimetric biosensor: Preliminary characterization
No full textWe present our preliminary results about the implementation of a new nanogravimetric biosensor realized combining Quartz Crystal Microbalance with Dissipation Monitoring Nanogravimetry and an innovative protein cell-free expression system named Nucleic Acid Programmable Protein Arrays (NAPPA) that allowed us to immobilize on the quartz surface, as sensing molecule, the proteins starting from its plasmid. We herby present results about the characterization of the nanogravimetric biosensor, its sensitivity, and its selectivity. Moreover we demonstrated how the simultaneous measurements of changes in resonance frequency and in energy dissipation were fundamental to correctly interpret the behavior of complex system such as NAPPA. © 2013 Elsevier B.V
Synchrotron Diffraction of Multilayered LS PGA Films After Heating And Cooling
No full textThe dramatic increase of long-range order in the LB multi-layered enzyme films after heating and cooling, made previously apparent by grazing incidence small angle X-ray scattering using ID13 microbeam [1], is here confirmed by these synchrotron diffraction studies at the ID11 beamline, opening the way to LB technology for bypassing the bottleneck of protein crystallization for protein structure determination which is leaving still unsolved large part of important proteins , like the membrane one
Analysis of gene expression on anodic porous alumina microarrays
No full textThis paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed by pen robot tips under standard conditions. The gene expression within the Nucleic Acid Programmable Protein Array (NAPPA) is realized in a confined environment of 16 spots, containing circular DNA plasmids expressed using rabbit reticulocyte lysate. Authors demonstrated the usefulness of APA in withholding the protein expression by detecting with a CC D microscope the photoluminescence signal emitted from the complex secondary antibody anchored to Cy3 and confined in the pores. Friction experiments proved the mechanical resistance under external stresses by the robot tip pens printing. So far, no attempts have been made to directly compare APA with any other surface/substrate; the rationale for pursuing APA as a potential surface coating is that it provides advantages over the simple functionalization of a glass slide, overcoming concerns about printing and its ability to generate viable arrays. © 2013 Landes Bioscience
Gene expression in the cell cycle of human T-lymphocytes: II. Experimental determination by DNASER technology
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