1,721,042 research outputs found
Coesistenza di Klebsiella pneumoniae ed Escherichia coli kpc-3-produttori in una paziente in terapia intensiva.
No full textIn vitro activity of ceftaroline against a collection of hospital-acquired MRSA (ha-MRSA) and ha-MSSA italian isolates.
No full textDifferences in biofilm formation and aggregative adherence between beta-lactam susceptible and beta-lactamases producing P. mirabilis clinical isolates.
No full textBiofilm formation of multidrug resistant (MDR) extended-spectrum beta-lactamase (ESbetaL) producing Proteus mirabilis isolates from a long-term care and rehabilitation facility (LTCRF) in Northern Italy was evaluated. A total of 10 strains, 4/10 producing the acquired AmpC beta-lactamase CMY-16, 3/10 producing the ESbetaL TEM-92 and the remaining negative for the presence of beta-lactamase genes, were studied using standard adherence assays on titer plates. Tests were performed in three different media, including Luria Bertani (LB), LB diluted and urine. Three representative strains were also tested for biofilm production in microtiter in presence of beta-lactam sub-MIC concentrations. The same isolates were screened for aggregative adherence onto monkey kidney cells (LLC-MK2). All strains studied were capable of biofilm formation, though at different levels. The beta-lactamase positive strains were statistically better significant in biofilm formation than negative ones regardless of growth medium. Cellular adherence assays showed a preferential ability of all isolates, regardless of beta-lactamase production, to adhere to inert surfaces rather than to cells. Although the results did not fully support a direct correlation between beta-lactamase production and biofilm formation, both mechanisms can greatly contribute to bacterial persistence in the urinary tract
Molecular epidemiology of ESβL producing P. mirabilis strains from a long-term care and rehabilitation facility in Italy
No full textWe report the detection of multidrug resistant ESbetaL producing Proteus mirabilis isolates from a long-term care and rehabilitation facility (LTCRF) in Northern Italy. 53% of the collected P. mirabilis strains were ESbetaL producers. PCR and sequencing techniques confirmed the presence of the bla(TEM-92) and bla(CMY-16) resistance genes in 23/26 (88.5%) and 3/26 (11.5%) of the ESbetaL producers respectively. PFGE showed that the TEM-92 beta-lactamase producing isolates were not clonally related, indicating the presence of at least four different clonal lineages (A, B, C, D), whereas all the CMY-16 enzyme producers belonged in the same lineage. The bla(TEM-92) and bla(CYY-16) determinants were distributed in seven different wards, but in three of them they coexisted. Our results show that the most patients are co-colonized by ESbetaLs producing P. mirabilis strains at the time of admission to an LTCRF. An effective strategy to curtail the spread of ESbetaLs mediated resistance in LTCRFs could be to activate sourveillance programs to monitor routinely the entry of resistant bacteria
PERSISTENZA DI ISOLATI DI ACINETOBACTER BAUMANNII MDR RESPONSABILI DI EPIDEMIE IN TRE OSPEDALI ITALIANI
No full textUtilizzo dei pannelli Microscan per valutare, in accordo con i breakpoint clinici EUCAST, la sensibilità antimicrobica di isolati Gram-negativi produttori di β-lattamasi.
No full textValutazione del test cromogeno b-lactatm per l’identificazione rapida di Enterobatteri resistenti alle cefalosporine ad ampio spettro.
No full textRecurrent vulvovaginal candidiasis: prevalence, antifungal susceptibility patterns and genotyping of different species
No full text
- …
