507 research outputs found
Chemical composition and antibacterial activity of essential oils from Daucus sahariensis Murb. roots and stems
No full textDaucus genus (Apiaceae) comprises economically relevant plants distributed in temperate regions. These plants are used mainly as food but are also known for their biological properties. Here, the essential oils (EOs) obtained by hydro-distillation of roots and stems of D.sahariensis Murb. growing in Algerian Sahara were studied by GC-MS analysis. Fifty-two and fifty-five compounds were identified from the roots and stems, representing 95.1% and 93.8% of the whole EOs respectively. The main constituents of the EO from roots were myristicin (29.2%), (E)-anethole (14.2%), cis-chrysanthenyl acetate (8%) and estragol (7.5%). In the EO from stems, the main constituents were α-pinene (18.6%), myrcene (16.7%), limonene (13.7%) and myristicin (8%). The antibacterial activity of EOs was evaluated by disc diffusion method and tested against Gram-positive and Gram-negative bacteria. Results showed a mild activity against Bacillus subtilis and Klebsiella pneumoniae for both EOs, which can be potentially used as antibacterial in phytotherapy
GC–MS analysis of essential oil from the leaves of Algerian Bupleurum plantagineum Desf
No full textBupleurum plantagineum Desf. (Apiaceae) is a plant endemic to North Africa, including Algeria. It is used as natural medicine, due to its anti-inflammatory, antioxidant and hepatoprotective properties. In this work, we studied the chemical composition of the essential oil (EO) obtained by hydro-distillation from B. plantagineum leaves collected from the national park of Gouraya (Bejaia), Algeria. Thirty components were identified in the EO by Gas chromatography-Mass Spectrometry(GC-MS) (relative abundance: 96.7%). Monoterpene hydrocarbons (56.8%) were the predominant, followed by oxygenated monoterpenes (36.7%). The main components were cis-chrysanthenyl acetate (33.5%), alpha-pinene (18.4%), myrcene (16.5%) and (E)-anethole (4.9%). This volatile profile differs from those reported for other Bupleurum spp. and for B. plantagineum from Algeria, suggesting the identification of a new chemotype. Overall, our results represent a contribution to the characterisation of natural products from Algeria and the preservation of its natural biodiversity. The same results will also represent a starting point for further studies on B. plantagineum
Assessment of Antioxidant effect of the essential oil and methanol extract of Centaurea dimorpha Viv. aerial parts from Algeria
No full textThe essential oils obtained by hydrodistillation of aerial parts of Centaurea dimorpha Viv. were analyzed by means of gas chromatography-mass spectrometry (GC-MS). The main constituents were Caryophyllene oxide (09.88%), limonene (5.73%), tetradecanoic acid (5.68%), spathulenol (5.44%), methyl hexadecanoate (4.45%), and α-pinene (03.08%). To our best knowledge this is the first study of essential oils from the aerial parts of Centaurea dimorpha growing in Algeria. Moreover the essential oil and methanolic extract were screened for their possible in vitro antioxidant activity by DPPH free radical-scavenging test. The findings showed that the percentage inhibition is 10.67 and 77.01 respectively at a concentration of 1mole/L
Deciding on authorship
No full text[Extract] Now that you have read the guidelines for authors and decided that your work is ready for submitting to a suitable journal. Yes, the next step is to decide on authorship. I mean to say "whose name will appear in the manuscript and in what order". It is not at all a problem (issue) when you have worked alone where you will be the sole author in the manuscript. If at all there are multiple authors it is the best time to decide who will be the authors for the publication. This will prevent later arguments. Rational colleagues have become bitter enemies because of difference of opinions on the matter that whose names should be included in the paper and in what order
Preparing to write a paper
No full text[Extract]
• Once you are about to start writing a paper you have to first select the journal you intend to send your article
• Once you select the journal it is important to determine if there are any special requirements of the journal. Read the instructions to
author. It usually differs from journal to journal but there is no much difference. Certain journals may specify the rules of nomenclature or certain style of writing. Do it accordingly.
• If your study includes experiments on human beings or animals then ethical committee clearance is a must . You would have got the clearance before you conduct the study. Keep the certificate ready.
• Most of the journals prefer the use of international system for units of measurements [SI units]. If it specifies then use the SI units only
Physically interpretable machine learning methods for transcription factor binding site identification using principled energy thresholds and occupancy:
No full textRegulation of gene expression is pivotal to cell behavior. It is achieved predominantly by transcription factor proteins binding to specific DNA sequences (sites) in gene promoters. Identification of these short, degenerate sites is therefore an important problem in biology. The major drawbacks of the probabilistic machine learning methods in vogue are the use of arbitrary thresholds and the lack of biophysical interpretations of statistical quantities. We have developed two machine learning methods and linked them to the biophysics of transcription factor binding by incorporating simple physical interactions. These methods estimate site binding energy, recognizing that it determines a site's function and evolutionary fitness. They use the occupancy probability of a transcription factor on a DNA sequence as the discriminant function because it has a straightforward physical interpretation, forms a bridge between binding energy and evolutionary fitness, and has a natural threshold for classifying sequences into sites that allows establishing the threshold in a principled manner. Our methods incorporate additional characteristics of sites to enhance their identification. The first method, based on a hidden Markov model (HMM), identifies self-overlapping sites by combining the effects of their alternative binding modes. It learns the threshold by training emission probabilities using unaligned sequences containing known sites and estimating transition probabilities to reflect site density in all promoters in a genome. While identifying sites, it adjusts parameters to model site density changing with the distance from the transcription start site. Moreover, it provides guidance for designing padding sequences in experiments involving self-overlapping sites. Our second method, the Phylogeny-based Quadratic Programming Method of Energy Matrix Estimation (PhyloQPMEME), integrates evolutionary conservation to reduce false positives while identifying sites. It learns the threshold by solving an iterative quadratic programming problem to optimize the distribution of correlated binding energies of neutrally evolving orthologous sequences while restricting the values of binding energies of known sites and their orthologs. We have used the NF-κB transcription factor family as a case study for both methods and gained new insights into its biology.Ph.D.Includes bibliographical references (p. 210-226)by Amar Mohan Drawi
Political Party and Senatorial Succession: A Response to Vikram Amar on How Best to Interpret the Seventeenth Amendment
No full textThis Article explores the author\u27s fundamental disagreement with Vikram Amar\u27s argument as to the meaning of the Seventeenth Amendment. Amar\u27s argument is a brilliant explication of various trees that misses the reality of the surrounding forest. The author contrasts my response to this article to another article that several years ago definitively demonstrated the unconstitutionality of the current Succession in Office Act (the Act) inasmuch as it makes the Speaker of the House (and then the president pro tempore of the Senate) next in line to the vice president to fill any vacancies in the Oval Office. Both that article and the one under discussion here are alike in making skillful use of the methods, including the particular fillip identified as intratextuality. Yet the first article was absolutely convincing, while the one examined here leaves the author decidedly skeptical
Political Party and Senatorial Succession: A Response to Vikram Amar on How Best to Interpret the Seventeenth Amendment
No full textThis Article explores the author\u27s fundamental disagreement with Vikram Amar\u27s argument as to the meaning of the Seventeenth Amendment. Amar\u27s argument is a brilliant explication of various trees that misses the reality of the surrounding forest. The author contrasts my response to this article to another article that several years ago definitively demonstrated the unconstitutionality of the current Succession in Office Act (the Act) inasmuch as it makes the Speaker of the House (and then the president pro tempore of the Senate) next in line to the vice president to fill any vacancies in the Oval Office. Both that article and the one under discussion here are alike in making skillful use of the methods, including the particular fillip identified as intratextuality. Yet the first article was absolutely convincing, while the one examined here leaves the author decidedly skeptical
Correction to: Exome sequencing reveals a novel PLP1 mutation in a Moroccan family with connatal Pelizaeus-Merzbacher disease: a case report
No full textAfter publication of the original article [1] it was brought to our attention that author Bouchra Ouled Amar Bencheikh was incorrectly included as Bouchra Oulad Amar Bencheikh
Ecriture Et Figures De Style Dans Les Nouvelles De Amar Mezdad, Le Cas De Tuɣalin Et Inebgi N Yiḍ-nni
No full textRésumé: Nous allons voir dans cet article, l’écriture de l’auteur Amar Mezdad dans les nouvelles «Tuɣalin» et «Inebgi n yiḍ-nni», comme les quelques figures de style employées, les comparaisons, les métaphores et les périphrases. Ainsi que les quelques formules anciennes puisées dans la tradition orale. Nous allons voir aussi le procédé d’accumulation utilisé par l’auteur afin d’enrichir son texte. Abstract: We will see in this article, writing of author Amar Mezdad in his short stories «Tuɣalin» et «Inebgi n yiḍ-nni», like some figures of speech used, comparisons, metaphors and paraphrases. As well as a few old formulas derived from the oral tradition. We will also see the process of accumulation used by the author to enrich the te
- …
