1,720,994 research outputs found
Thermal Behavior of Two Recombinantly Expressed Human Elastin-Like Polypeptides
No full textTwo synthetic genes that code for artificial proteins modeled on the most regularly repeated hydrophobic domain of human tropoelastin have been constructed. We compared physicochemical properties of the recombinant products. The data presented in our work support the usefulness of these versatile biopolymers for a variety of applications related to biotechnology and biomedicine
Comportamento di Polimeri in Presenza di CO2 ad Alta Pressione
No full textProceedings (senza ISBN) del Congresso "Congresso GRICU 1998: Formazione e Ricerca", Ferrara, 23-25 settembre 1998
Experimental Measurement of the Sorption and Swelling of Polymers in Presence of Supercritical Carbon Dioxide
No full textPoster 112ae presentato all'AIChE Annual Meeting, Miami (FL - USA), 15-20 novembre 199
Fluorometric Nanoscale Analysis of Bilirubin and Biliverdin in Human Cerebrospinal Fluid
Full text linkCerebrospinal fluid (CSF) is a valuable source for the quantification of soluble, brain-specific biomarkers supporting the diagnosis of some neurological disorders, infectious diseases, and suspected subarachnoid hemorrhage. Given the increasing need to expand the basic knowledge of brain pathophysiology for disease biomarker discovery, we set the goal to quantify bilirubin and biliverdin in human CSF. Their concentrations are expected to reflect the level of brain heme catabolism, a key pathway involved in the biological response to oxidative stress. Here, we present the results of the CSF analysis by a specific and sensitive fluorometric method using the recombinant fusion protein HELP-UnaG (HUG). The concentrations of bilirubin and biliverdin in 50 human CSF samples were in the ranges of 14-340 and 0-66 nM, respectively. This assay can be easily implemented in small-scale laboratories and neurological units for the routine analysis of clinical CSF samples
Phase transition and particle formation of a Human Elastin-Like polypeptide
No full textElastin-Like Polypeptides are an interesting class of
recombinant proteins that mimic elastin. Here we present some
data about phase transition properties and particle formation
features of the recombinantly expressed Human Elastin-Like
polypeptide (HELP). This macromolecule shows a promising
potential as vehicle for delivery and controlled release of active
compounds
Indagine dell'esposizione a polveri ed inquinanti atmosferici di lavoratori outdoor nell'area di Muggia (Trieste)
No full textL’inquinamento urbano di origine veicolare,
domestico ed industriale espone varie categorie di lavoratori
a molteplici agenti chimici dannosi per la salute per cui negli
ultimi anni sono state effettuate diverse indagini su vigili urbani,
taxisti, guidatori di autobus, poliziotti, edicolanti, benzinai e sulla
popolazione generale per una stima dell’esposizione ai principali
contaminanti, soprattutto IPA e benzene.
Obiettivo. Definire una specifica strategia di campionamento
per ottenere delle basi sperimentali sull’esposizione potenziale
della popolazione a polveri sospese nell’area comunale
di Muggia (TS).
Materiali e metodi. È stato analizzato un campione di 14 vigili
urbani occupazionalmente esposti che svolgono la loro
professione prevalentemente “in strada” e un campione di
13 amministrativi comunali (controllo) che svolgono la loro
professione, nelle stesse ore, in ambiente confinato (ufficio).
L’esposizione è stata misurata mediante campionatori
personali e campionatori ambientali ad alto volume. Si sono
inoltre determinati i livelli di 1-idrossipirene e creatinina nei
campioni di urine prelevati da tutti i soggetti in esame.
Risultati. L’analisi degli IPA ha fornito rapporti diagnostici
per l’individuazione delle sorgenti inquinanti. In generale
i valori di BaP ottenuti si mantengono al di sotto del limite
previsto, con buon accordo tra il monitoraggio ambientale e
quello personale. Il dosaggio dell’1-OH pirene rimane entro
il valore di fondo (0.089 μmoli/mole di creatinina) per il
personale amministrativo, mentre la maggior parte dei dati
relativi ai vigili, pur ampiamente al di sotto del valore
di prudenza risultano superiori
Sorption of Supercritical Carbon Dioxide in Biocompatible Polymers: Experimental Data and Qualitative Interpretation
No full textProceedings (su CD e senza ISBN)della Conferenza "8th International Conference on Properties and Phase equilibria for Product and Process design", Noordwijkerhout (NL), 26 aprile - 1 maggio 1998. -
Sorption of CO2 (at 308.3 and 320.1 K in the pressure range of 0-120 bar) in different biocompatible polymers (ethyl-cellulose, carboxy-methyl-cellulose, and polyvinyl-pyrrolidone) has been measured by gravimetric method. The experimental data are qualitative interpreted in terms of Gibbs free energy of mixing: in particular, the role of enthalpic and entropic effects are taken into account
Extracellular carbohydrates released by the marine diatoms Cylindrotheca closterium, Skeletonema costatum and Thalassiosira pseudonana: effect of P-depletion and growth status
No full textA laboratory study was performed on the extracellular production of carbohydrates by the marine diatoms Cylindrotheca closterium, Thalassiosira pseudonana and Skeletonema costatum. The investigation was aimed at elucidating the role of Pstarvation and growth status on abundance and chemical characteristics of the released non-attached polysaccharides. Inorganic phosphorus depletion determined an increase of total polysaccharides in all species examined compared to nutrient-replete (complete f/2) conditions. The highest abundance of polysaccharides per unit cell was found in T. pseudonana (28.4 Amol C 106 cells), followed by C. closterium (2.56 Amol C 106 cells) and S. costatum (1.18 Amol C 106 cells). Maximum production rates were found at the transition between exponential and stationary growth phase
Abundance and chemical characterization of Extracellular Carbohydrates Released by the Marine Diatom Cylindrotheca fusiformis under N- and P-limitation
No full textThe effect of P- and N-depletion on the production and molecular-level composition of extracellular carbohydrates released by the diatom Cylindrotheca fusiformis was examined in axenic batch cultures. Inorganic phosphorus depletion caused a higher release of organic carbon per cell compared to nutrient-replete and nitrogen-limited conditions. Gas-chromatographic analysis of the monosaccharide composition of the dissolved fraction showed galactose to be the major component of the exopolymers (33 – 42%). In the P-limited treatment, an increase of galactose content and a decrease of glucose was found, while the composition of the remaining saccharides was almost unaffected. The results point out the relevance of phosphorus limitation not only in causing an increase in total exopolysaccharide production, but also in affecting the neutral aldose composition of the dissolved carbohydrates released by C. fusiformis, with heteropolysaccharides being more abundant than glucan
- …
