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Listroscelis cyanotibiatus Mendes & Silva-Neto 2023, sp. nov.

Author: Mendes Diego Matheus De Mello
Neto Alberto Moreira Da Silva
Publication venue
Publication date: 13/03/2023
Field of study

Listroscelis cyanotibiatus Mendes & Silva-Neto sp. nov. Figures 1–7 Diagnosis. The turquoise blue coloring of tibiae and part of tegmina makes this katydid unique and easily differentiated from other species of Listroscelis (Fig. 6). Tegmina long, surpassing apex of cercus and subgenital plate, in lateral view (Fig. 1A). Cercus fang-shaped, apically with inward curved tip; apex of cercus not exceeding base of stylus (Figs. 1I–K). Description. Holotype Male. Head. Head laterally wide, with frons and gena rough, in frontal view (Figs. 1B–C); fastigium-vertex small and laterally compressed, in frontal view (Fig. 1B); Globular eyes (Figs. 1B and 1D). Scape and pedicel wide and cylindrical, in lateral view (Fig. 1D). Mandibles asymmetrical; left mandible very elongate, ensiform, acuminate and projecting forward; right mandible curved and not elongated (Figs. 1A–C). Thorax. Pronotal disc rectangular, without carina, anteriorly slightly concave and posteriorly straight, in dorsal view (Fig. 1E); dorsal margin of pronotal disc with undulations, in lateral view (Fig. 1E); Dorsal-lateral suture curved and bifurcate (Figs. 1D–E). Furcal suture curved and concolorous with pronotal disc (Fig. 1E). Mesobasisternum triangular-shaped, anteriorly slightly concave and posteriorly narrow (Fig. 1E). Lateral lobes of mesobasisternum anterolaterally and laterally convex and posteriorly straight (Fig. 1E). Metabasisternum trapezoidal (Fig. 1E). Lateral lobes of mesobasisternum laterally slightly convex and posteriorly rounded (Fig. 1E). Mesobasisternum and metabasisternum with two rounded projections on each (Fig. 1E). Wings. Tegmina narrow with rounded apex, with about 1/3 of the tegmina surpassing the apex of the cerci (Figs. 1A, 2A–B). Left stridulatory file sinuous; teeth apical minor in relation to middle teeth (Fig. 3A). Total length of left stridulatory file 4 mm, greater vein width of 0.2 mm and a total of 178 teeth (Fig. 3A). Right stridulatory file sinuous; teeth basal and apical shorter than middle teeth (Fig. 3B). Total length file of 2 mm, widest vein of 0.1 mm and total of 156 teeth (Figs. 3B). Legs. Fore femur straight, with apex narrower than the base, presence of four ventral spines, a pair of apical spines and numerous small ventral spines, in lateral view (Fig. 1F). Fore tibia sinuous with six pairs of long spines in ventral region, in lateral view (Fig. 1F). Mid femur curved, with four pairs of large spines and numerous small spines on ventral margin, in lateral view (Fig. 1G). Mid tibia with enlarged basal half with six pairs of ventral spines and three dorsal spines, in lateral view (Fig. 1G). Hind femur enlarged at basal half, with several large ventral spines (Figs. 1A). Hind tibia straight, narrow, with several small ventral and dorsal spines (Figs. 1A). All legs with short bristles (Figs. 1A, F–G). Abdomen. Cercus fang-shaped, apically with inward curved tip; apex of cercus not exceeding base of stylus (Figs. 1I–K). Subgenital plate trapezoidal and posteriorly bifurcated (Figs. 1I–K). Space concave between the base of stylus (Figs. 1I–K). Stylus long and narrow (Figs. 1I–K). Internal male genitalia. Upper fold of ventral lobe large, asymmetric; margins slightly sinuous (Figs. 4A–D). Titillator with two large sclerites, parallel, inward curved and anteriorly exceeding the dorsal lobe (Figs. 4A–D). Sclerite of ventral fold of dorsal lobe with one large sclerite, sinuous, narrow medially and wide distally; apex acuminate (Figs. 4A–D). Lower fold of ventral lobe small, bilobed, posteriorly not exceeding the upper fold of ventral lob (Figs. 4A–B). Dorsal lobe anteriorly with median projection large elongated, anterolaterally with two convex projections, laterally and posteriorly slightly rounded (Figs. 4A–D). Dorsal fold asymmetric, elongated and narrow (Figs. 4C–D). Coloration. Described based on photos of live specimens (Fig. 6). Scape, pedicel dark brown and antennal sclerite light brown. Eyes dark brown. Head, pronotal disc, fore leg and mid leg dark brown with some light brown areas. Jaws black. Tibiae with black brown base and apex and turquoise blue middle region, with black spines. Tarsi dark brown. Hind femur light brown with apex dark brown. Thorax light brown with some dark brown areas. Tegmina dark brown; basal region of the wing a large blue-white and posteriorly turquoise-blue spot slightly hyaline between the costal margin and the main branch of vein R. Stridulatory region of tegmina light brown. Abdominal segment dorsally dark brown with a turquoise blue lateral band. Cerci light green. Female: Similar to male, except for the following features (Fig. 5): Wings. Anal veins unmodified for sound production. Abdomen. Cercus slightly inward curved with acuminate and inward curved apex (Figs. 5H–J). Cercus with long white bristles (Fig. 5H). Subgenital plate triangular, anteriorly straight and posteriorly slightly bilobate (Fig. 5I). Ovipositor slightly curved, without teeth, with enlarged base and subsequently narrow to the apex; apex of ovipositor triangular (Fig. 5J). Coloration. Ovipositor with light brown base and other dark brown regions. Etymology. The epithet is from Latin, cyano (blue) + tibia (tibia, leg) + atus (provided with), meaning “provided with blue tibia”, in reference to the striking and diagnostic blue coloration of the legs of this katydid. Geographical records. Brazil: Bahia (Fig. 7). Type material. Holotype ♁. BRASIL, Bahia, Cachoeira, Fazenda Vila Rial, 19.v.2018, D.M.M. Mendes & A.M.S. Neto leg. (INPA). Paratypes: Idem, Itanagra, 19.ii.2021, A.M.S. Neto leg. (1&female; INPA); Idem, Santa Teresinha, Serra da Jiboia, Base Gambá, 12°52′19.8″S – 39°28′51.7″W, 08–10.v. 2018, 500 m de altitude, coleta manual, D.M.M. Mendes & A.M.S. Neto leg. (1&female; INPA). Measurements (mm). Holotype♁: TL: 52; TegL: 50; TegH: 8; WF: 4,5; PL: 5,3; PH: 3,2; FF: 10; FT: 10,5; MF: 6,7; MT: 7,5; HF: 16; HT: 18; Lplac: 3,5; LC: 2,8. Paratypes &female;: TL: 52; TegL: 50; TegH: 8; WF: 4,5; PL: 5,3; PH: 3,2; FF: 10; FT: 10,5; MF: 6,7; MT: 7,5; HF: 16; HT: 18; Lplac: 3,5; LC: 2,8; OV: 3. Comments. The state of Bahia currently has records of three species of Listroscelis: Listroscelis magnomaculata Fialho et al., 2014, Listroscelis monnei Fialho et al., 2014, Listroscelis cohni Fialho et al., 2014. L. cyanotibiatus sp. nov. appears to be closer to L. magnomaculata (differentiating by long tegmina versus short tegmina, not exceeding the apex of cercus in L. magnomaculata).Published as part of Mendes, Diego Matheus De Mello & Neto, Alberto Moreira Da Silva, 2023, The Blue Legged Predatory Katydid-a new species of Listroscelis Serville, 1883 (Orthoptera: Tettigoniidae: Listroscelidinae: Listroscelidini) from the Atlantic Rainforest, pp. 425-433 in Zootaxa 5254 (3) on pages 426-430, DOI: 10.11646/zootaxa.5254.3.8, <a href="http://zenodo.org/record/7727752">http://zenodo.org/record/7727752</a&gt

ZENODO

Neto-β, but not Neto-α, restores PAK recruitment and mEJP amplitude at netonull NMJs.

Author: Oghomwen Igiesuorobo (696508)
Mihaela Serpe (696510)
Qi Wang (22418)
Cathy I. Ramos (696509)
Publication venue
Publication date: 2015
Field of study

(A) Confocal images of larval NMJ4 labeled for Neto-ex (red), PAK (green) and HRP (blue). Neto-β, but not Neto-α, restores PAK synaptic accumulation over a large range of concentrations tested. (B) Western blot comparison of Neto levels in muscle extracts from control (first lane), and netonull larvae rescued with neto-α transgenes (low, medium, and high expression) (magenta gradient), or neto-β transgenes (low, medium, high, and very high expression)(blue gradient). Arrows indicate unprocessed and processed Neto-α (magenta) and Neto-β (blue). (B’)—low exposure. Tubulin was used as a loading control. (C-H) Electrophysiological recordings of netonull NMJs rescued by various levels of Neto-α or Neto-β. Data are reported relative to controls matched by rearing at 18°C (empty bars) or 25°C (hatched bars). Representative traces of mEJPs and EJPs are shown in (C) and (F), respectively. The mEJPs amplitude is reduced at NMJs rescued by low and medium levels of Neto-α (D). The mEJPs frequency appears less dependent on Neto levels/isoforms, but is significantly increased in larvae reared at 18°C (E). The EJPs amplitudes are largely normal (G), likely due to subtle, but significant increases in quantal content at Neto-α-rescued NMJs (H). (I) Confocal images of NMJ4 boutons (segment A3) in control (w1118) and GluRIIASP16/Df (GluRIIASP16/ Df(2L)clh4) third instar larvae labeled for Neto-ex (red), PAK (green), and GluRIIA (blue). PAK is normally present at GluRIIA mutant synapses, indicating that the synaptic recruitment PAK does not depend on GluRIIA. Genotypes: control (G14-Gal4/+); netoα low (neto36/Y; G14-Gal4/UAS-neto-A9), reared at 25°C); netoα med (neto36/Y; G14-Gal4/UAS-neto-A3, 18°C); netoα high (neto36/Y; G14-Gal4/UAS-neto-A3, 25°C); netoβ low (neto36, UAS-neto-B6/Y; G14-Gal4/+, 18°C); netoβ med (neto36, UAS-neto-B6/Y; G14-Gal4/+, 25°C); netoβ high (neto36/Y; G14-Gal4/UAS-neto-B3, 18°C); netoβ very high (neto36/Y; G14-Gal4/UAS-neto-B3, 25°C). Error bars indicate SEM. ***; p<0.001, **; p<0.005, *; p<0.05, ns; p>0.05. Scale bars: 20 μm, 2 μm in details and (I).</p

The Francis Crick Institute

Parentocirrus brasiliensis Paiva & Silva-Neto 2004, sp. n.

Author: Silva-Neto Inácio Domingos Da
Paiva Thiago Da Silva
Publication venue
Publication date: 04/05/2004
Field of study

Description of Parentocirrus brasiliensis sp. n. Derivatio nominis Because of the country in which this species was found. Locus typicus Samples of activated sludge from Estação de Tratamento de Esgoto da Penha (ETE – Penha), a wastewater treatment plant from Companhia Estadual de Águas e Esgotos (CEDAE/RJ), located in Penha, Rio de Janeiro, Brazil. a Mean = arithmetic mean; b M = median; c SD = standard deviation of the arithmetic mean; d SE = standard error of the arithmetic mean; e CV = coefficient of variation; f Min = minimum value observed; g Max = maximum value observed; h n = sample size. Diagnosis Size in vivo about 110 x 75µm. Body elliptical, with a single round shaped contractile vacuole in the equatorial region, close to the left margin. The ventral ciliature is composed of three distinct enlarged anterior frontal cirri, followed by a set of 2–3 buccal cirri and 3– 5 posterior frontal cirri of the same size; two marginal cirral rows, two rows of ventral cirri ending in an oblique row of 3–4 transverse cirri, and 2–4 postperistomial cirri. Macronuclear apparatus composed of 4–6 macronuclear nodules and 1–6 micronuclei. Dorsal side shows 6 (rarely 5) kineties, with very few scattered kinetids between kineties 3 and 4. Kineties 1, 2 and 4 ends each one in a caudal cirrus. Feeds on smaller protists and bacteria. Morphological Characterization This new species has conspicuous adoral zone of membranelles, composed of 32–49 membranelles and occupies about 43% of average cell length, the membranelles in the transversal region are about 18µm long. The first 2 or 3 proximal membranelles are engulfed by an oral lip. The paroral and endoral membranes virtually intercept each other in their middle section, displaying the usual “oxytricha pattern” (Berger & Foissner, 1997). All studied specimens show three enlarged anterior frontal cirri, which are easily recognizable from the rest of the ventral ciliature, due to their position and constant size. Below this cirral set is a complex of buccal and posterior frontal cirri that are somewhat difficult to distinguish. In fact, we tried to follow the terminology adopted by Voss (1997), but we found that it may be inaccurate for P. brasiliensis, due to the variable number of cirri in this region, as well as their organization, which is different from P. hortualis. This complex is composed by 2–3 buccal cirri, in which the anteriormost is placed right and adjacent to the anterior section of the paroral membrane, and 3–5 posterior frontal cirri, which we considered as the cirri below the anterior frontal cirri, that are not placed adjacent to the undulating membranes and whose the distance from the first cirrus of the ventral rows is larger than the distance observed between the cirri on such rows (Figs. 1, 2, 4). The left ventral cirral row commences right of the posterior section of the endoral membrane, usually below the rightmost posterior frontal cirrus, extending to the transverse cirri set. The right ventral cirral row commences right of the rightmost anterior frontal cirri and ends in the rightmost cirrus of the transverse set, which is composed by an oblique row of 3–4 cirri. When in 4, the 2 leftmost transverse cirri are placed left of the ventral rows. The left marginal cirral row begins below the middle region of the adoral zone of membranelles. The right marginal cirral row begins at the level of the uppermost buccal cirrus. Both marginal rows are convergent in the anterior end of cell, but do not fusionate, terminating at the same level. The cirri in the marginal rows measure about 15µm in the equatorial region of the cell. In the dorsal surface, we observed the occurrence of six (5 in one specimen) dorsal rows of kinetids. Row three begins anterior to row two, and its orientation in the anterior end is opposite to that observed in P. hortualis. Also, there are few scattered kinetids present between rows 3 and 4, usually in small number (3 or 4 in most specimens). The posterior scattered kinetids seems to compose a loose short kinety that merges with the posterior region of kinety 3 (Figs. 5, 10). Kineties 5 and 6 are shortened, ending before the equatorial region of cell. Kineties 1, 2 and 4 bears each one a caudal cirrus about 15µm long (Figs. 3–7). The nuclear apparatus is composed of a row of 4–6 macronuclear nodules, which sometimes are distinctly spread into two sets. These sets are not linked by a connection bridge, as in P. hortualis. The nodules are spheroid in shape, and measure 12µm in average diameter. One to six micronuclei are attached to the macronuclear nodules. Their average diameter in the studied population is 2.5µm (Fig. 2). The whole nuclear apparatus is located along the central longitudinal axis of the body, tending to form an arch, in which the convex side bends to the left region of body. Typification A slide with the holotype and several paratypes of Parentocirrus brasiliensis was deposited in the collection of Lab. de Protistologia, Dept. de Zoologia, Inst. de Biologia, Universidade Federal do Rio de Janeiro – UFRJ. Access number: 0007–1. Discussion The presence of very few kinetids scattered on the dorsal surface may represent a close relationship with genus Apoamphisiella Foissner, 1997, a genus with both similar ventral ciliature organization and the presence of numerous scattered kinetids in the dorsal surface. Other possible related genera include Paraurostyla Borror, 1972 and Territricha Berger and Foissner, 1988, however, in Paraurostyla, the ventral pattern shows increased number of ventral cirral rows, although still resembles the organization present in Parentocirrus and Apoamphisiella, and in contrast, Territricha has two ventral cirral rows which are arranged in a single set of paired cirri. Another observation is that in some specimens measuring 90µm or less, the nuclear apparatus is composed of four or five macronuclear nodules instead of six, and that in these specimens, the nodules in both ends of the macronuclear row are elongated and constricted at the equatorial region (Fig. 8). Rare intrapopulational variants with anomalous nuclear apparatus are known to occur in some species of spirotrichous ciliates. Kattar (1970) observed it in populations of Urostrongylum caudatum (Kahl, 1932). In early dividers, we observed that the morphogenesis of the dorsal kineties occurred in the same way as described by Voss (1997) for P. hortualis, but we did not observe the occurrence of parental cirri rows, except for an uncommon isolated cirrus that was observed in only two interphasic specimens. When present, this cirrus was located between the right marginal row and right ventral row, above level of the basis of adoral zone of membranelles (Fig. 9). The presence of parental rows reported by Voss in specimens of P. hortualis was considered by Berger (1999) as a very rare condition. We regard this population as a novel species different from P. hortualis because of the arrangement of dorsal kineties, the presence of scattered kinetids between kineties 3 and 4, the relative position of the posterior frontal cirri, the average number of macronuclear nodules, the number of postperistomial cirri, and the average body size. This species probably is conspecific with another population of P. hortualis found by Blatterer (unpublished data) and mentioned in Berger (1999), which was found in activated sludge samples from a papermill in Salzburg, Austria. This population of P. hortualis shows similarities with Parentocirrus brasiliensis sp. n. in organization of the posterior frontal cirri and the number of postperistomial cirri. However, the number of macronuclear nodules exceeds by three the maximum we observed (Table 1), and the contractile vacuole is located above the equatorial region of the cell, almost adjacent to the adoral zone of membranelles. Due to the lack in sufficient data about this population (there is only a single drawing of a living specimen showing the ventral surface available), we consider it incipient to synonymize them. Furthermore, because of the dorsal morphogenesis pattern, the ventral cirral configuration, and the overall similarities with Apoamphisiella and related genera, we agree with Berger (1999) that this genus should be placed within the Oxytrichidae, instead of the Kahliellidae, as originally proposed by Voss (1997).Published as part of Paiva, Thiago Da Silva & Silva-Neto, Inácio Domingos Da, 2004, Description of Parentocirrus brasiliensis sp. n. (Ciliophora: Spirotrichea), a new ciliate protist present in activated sludge, pp. 1-10 in Zootaxa 504 (1) on pages 2-9, DOI: 10.11646/zootaxa.504.1.1, <a href="http://zenodo.org/record/5028602">http://zenodo.org/record/5028602</a&gt

ZENODO

FIGURE 9 in Morphology and morphogenesis of Strongylidium pseudocrassum Wang and Nie, 1935, with redefinition of Strongylidium Sterki, 1878 (Protista: Ciliophora: Stichotrichia)

Author: Silva-Neto Inácio Domingos Da
Paiva Thiago Da Silva
Publication venue
Publication date: 24/08/2007
Field of study

FIGURE 9. Micrographs of Strongylidium pseudocrassum after protargol-impregnation showing aspects of physiological reorganization (a–c) and scanning electron images (d–g).Published as part of Paiva, Thiago Da Silva & Silva-Neto, Inácio Domingos Da, 2007, Morphology and morphogenesis of Strongylidium pseudocrassum Wang and Nie, 1935, with redefinition of Strongylidium Sterki, 1878 (Protista: Ciliophora: Stichotrichia), pp. 31-57 in Zootaxa 1559 (1) on page 44, DOI: 10.11646/zootaxa.1559.1.2, <a href="http://zenodo.org/record/10088405">http://zenodo.org/record/10088405</a&gt

ZENODO

Poemas atribuidos a Silva

Author
Publication venue
Publication date: 01/01/1979
Field of study

Se incluyen cinco poemas atribuidos a Silva: "Rien du tout", publicado por primera vez en ECI, tomo XVIII, 1909, pág. 534; "Viejo Rosal", publicado por primera vez en P, año I, vol. II, No. 35, mayo 28 de 1925; "¿Para qué quieres versos?", incluido por Roberto Liévano en su artículo sobre Silva en la RCh, julio de 1922, pág. 296; "Armonías", publicado por la revista Pan, agosto de 1938; "Nidos", recogido por Donald McGray. Los textos incluidos en éste documento HTML fueron tomados de: Silva, José Asunción: Poesía y prosa con 44 textos sobre el autor. Edición a cargo de Santiago Mutis Durán and J. G. Cobo Borda. Bogotá: Instituto Colombiano de Cultura, 197

Universidad Nacional De Colombia - Repositorio Institucional UN

Megatympanon austroraptorum Mendes, Sobral & Silva-Neto 2023, sp. nov.

Author: Mendes Diego Matheus De Mello
Neto Alberto Moreira Da Silva
Sobral Rafael
Publication venue
Publication date: 06/04/2023
Field of study

Megatympanon austroraptorum Mendes, Sobral & Silva-Neto sp. nov. Figures 1, 5–9 Diagnosis. Pronotal disc posteriorly forming a triangular sharp projection in dorsal view (Fig. 5C). Tegmina ellipsoid with round apex (Fig. 6). Tegmina slightly longer than body, only with short portion of apex crossing the abdomen, in lateral view (Fig. 5A). Vein MA with four branch reaching apex of tegmina (Fig. 6). Cercus fang-shaped in dorsal view, conical in lateral view, apically with large tip curved inward (Figs. 5I–J). Subgenital plate trapezoidal, laterally concave and posteriorly bilobate with medial notch wide and V-shaped (Figs. 5I–K). Description. Holotype Male. Head. Head laterally wide, with frons and gena smooth, in frontal view (Fig. 5B); fastigium-vertex small and laterally compressed, in frontal view (Fig. 5B); Globular eyes (Figs. 5B–D). Mandibles symmetric (Figs. 5A–C). Thorax. Pronotal disc rectangular, without carina, anteriorly straight, laterally sharp and narrowing posteriorly, forming a triangular sharp projection in dorsal view (Fig. 5C); triangular posterior projection pointing upward, in lateral and frontal view (Figs. 5B and 5D); Dorsal-lateral suture curved (Figs. 5D–E). Furcal suture bifurcated and concolorous with pronotal disc (Fig. 5E). Mesobasisternum triangular-shaped, anteriorly concave and posteriorly narrow (Fig. 5E). Lateral lobes of mesobasisternum triangular, forming a sharp projection (Fig. 5E). Metabasisternum trapezoidal (Fig. 5E). Lateral lobes of mesobasisternum triangular, laterally slightly convex and posteriorly rounded, with medial projection acuminated and projected (Fig. 5E). Wings. Tegmina ellipsoid with round apex, presence of two big mirror cells in basal area (Fig. 6). Vein R slightly sinuous with innumerous branches almost straight reaching anterior margin of tegmina; last branch of Vein R reaching only the beginning of apical area of tegmina. Vein MA with basal half wide and slightly curved, posteriorly almost straight with two bifurcation of main branches at apical region; vein MP slightly curved, with two main branches reaching posterior margin of tegmina; one branch connecting to branches of vein CuA. Vein CuA narrow, anteriorly curved, surrounding area of second mirror cell and posteriorly with six branches; the first branch turned to base and the other turned to apex of tegmina. Vein CuP curved, large, surrounding posterior margin of first mirror cell. Vein AA narrow, surrounding area of first mirror cell. Vein Sc sinuous with many small branches. Left stridulatory file sinuous; teeth apical minor in relation to middle teeth (Fig. 7A). Total length of left stridulatory file 5 mm, greater vein width of 0.8 mm and a total of 54 teeth (Fig. 7A). Right stridulatory file sinuous, with basal half curved and apical half almost straight; basal half teeth short and increasing in size gradually to medial region; teeth on apical edges small (Fig. 7B). Total length file of 4 mm, widest vein of 0.7 mm and total of 55 teeth (Figs. 7B). Legs. Fore femur straight, with apical region slightly curved, presence of seven pairs of short ventral spines, being one pair apical and shorter and the others at medial region bigger than apical one, in lateral view (Fig. 5F). Fore tibia straight with six pairs of long ventral spines, thick and slightly curved, in lateral view; tympanic region slightly widened and with tympanic cavity almost completely closed, forming a short crevice (Fig. 5F). Mid femur straight, with apical region slightly curved, with seven of small ventral spines, in lateral view (Fig. 5G). Mid tibia straight with nine pairs of ventral spines long, thick and slightly curved, in lateral view (Fig. 5G). Hind femur elongated, narrow and enlarged at basal half, with several large ventral spines (Figs. 5A). Hind tibia straight, narrow, with several small ventral and dorsal spines (Figs. 5A). All legs with short bristles (Figs. 5A, F–G). Abdomen. Cercus fang-shaped in dorsal view, conical in lateral view, apically with large tip curved inward; apex of cercus not exceeding base of stylus; cercus with several short bristles (Figs. 5I–K). Subgenital plate trapezoidal, laterally concave and posteriorly bilobated with medial notch wide and V-shaped (Figs. 5I–K). Stylus long and narrow (Figs. 5I–K). Concealed male genitalia. Not examined. Coloration. Based on photos of live specimens (Fig. 8). Body mainly dark-green with smaller areas light green. Eyes dark brown with posterior strip greyish yellow. Scape greyish yellow; pedicel and the other antennomeres dark brown with black spots. Pronotal disc dark-green with posterior margin of lateral lobe with pale stripe. Tegmina dark-green; stridulatory area light brown with vein CuP pale anteriorly and light green posteriorly. Femora darkgreen with short spots light green; tibiae and tarsi light green with dorsal stripe orangish-pink. Abdominal segments light green. Female: Unknown. Etymology. The epithet is from Latin, austro (austral) + raptor (thief), meaning raptor austral, in reference to the predatory behavior of this katydid and to its distribution in southern Brazil. Geographical records. Brazil: Paraná, Santa Catarina and Rio Grande do Sul (Fig. 9). Type material. Holotype J. BRASIL, Santa Catarina, Rio Vermelho, ii.1950, Dirings leg. / MZSP 5366 (1J MZSP). Paratypes: Idem, ii.1960 / MZSP 5367 (1J MZSP); Idem, ii.1949 / MZSP 5365 (1J MZSP); Idem, 24.iii.1950 / MZSP 5368 (1J MZSP). Measurements (mm). HolotypeJ: TL: 29,8; TegL: 34,2; TegH: 12,3; WF: 5,6; PL: 10,0; PH: 4,2; FF: 13,0; FT: 13,3; MF: 12,9; MT: 13,0; HF: 31,8; HT: 31,5; Lplac: 4,0; LC: 3,1. ParatypesJ: TL: 29,2–28,7; TegL: 36,8–36,4; TegH: 11,4–10,1; WF: 5,8–5,6; PL: 11,2–10,1; PH: 5,2–4,5; FF: 12,4–10,2; FT: 13,9–13,5; MF: 12,3–12,2; MT: 13,9–13,8; HF: 32,0–31,4; HT: 33,1–33,0; Lplac: 5,0–4,8; LC: 3,2–2,8. Comments. The new species was found mainly in areas corresponding to the Serra do Mar coastal forests ecoregion and with one record in the Alto Paraná Atlantic forest ecoregion. The distribution in the Serra do Mar is a feature shared by both species of Megatympanon, but M. austroraptorum sp. nov. occurs in a southernmost portion than M. speculatum. Despite the near distribution, we did not find evidence of sympatry between both species. The Serra do Mar coastal forests are characterized by the coastal plains and chain of mountains, ranging from 20 m up to 2000 m of elevation. These differences in altitudes provide differences in climate and vegetation, which allows for great biological diversity in this ecoregion (Carlucci et al. 2021). The Serra do Mar is characterized by a great diversity of large tree species such as Copaifera trapezifolia, Pouteria spp., Chrysophyllum spp., epiphytes, and also species of Myrtaceae and Melastomataceae (Schipper, 2023). That habitat unfortunately has been highly fragmented by anthropic influence, with lowland forests depleted to the construction of urban areas and to slash-and-burn agriculture (Carlucci et al. 2021; Schipper, 2023).Published as part of Mendes, Diego Matheus De Mello, Sobral, Rafael & Neto, Alberto Moreira Da Silva, 2023, The Austral Predator Katydid-new species of Megatympanon Piza, 1958 (Orthoptera: Tettigoniidae: Listroscelidinae: Terpandrini) from the Brazilian Atlantic Rainforest, pp. 430-440 in Zootaxa 5263 (3) on pages 435-439, DOI: 10.11646/zootaxa.5263.3.7, <a href="http://zenodo.org/record/7804383">http://zenodo.org/record/7804383</a&gt

ZENODO

iGluRs synaptic accumulation is perturbed at neto-β mutant NMJs.

Author: Oghomwen Igiesuorobo (696508)
Mihaela Serpe (696510)
Qi Wang (22418)
Cathy I. Ramos (696509)
Publication venue
Publication date: 2015
Field of study

(A) Confocal images of NMJ4 boutons in larvae of indicated genotypes labeled for Brp (red), GluRIIC (green), and HRP (blue) (quantified in B-C). neto-β mutant NMJs have increased number of synaptic contacts. The intensity of the presynaptic active zone marker Brp appears to be normal, but the GluRIIC synaptic signals are reduced at neto-β mutant NMJs compared with control (precise excision). (D) Western blot comparison of GluRIIC protein levels in muscle lysates from neto-β third instar larvae. Tubulin was used as a loading control. (E) Confocal images of NMJ4 boutons in larvae of indicated genotypes labeled for GluRIIA (red), GluRIIB (green), and HRP (blue). The synaptic accumulation of GluRIIA is severely reduced at neto-β mutant NMJs (quantified in C). In contrast, the GluRIIB synaptic signals are slightly increased at netoβshort NMJs and significantly reduced (by 31%) at netoβnull NMJs. (F) Confocal images of NMJ4 boutons in control and neto-αRNAi larvae labeled for GluRIIA (red), GluRIIB (green), and HRP (blue). The GluRIIB signals, but not GluRIIA are reduced at Neto-α-depleted NMJs (quantified in G). (H) Western blot analysis of larval muscle extracts from netonull mutants rescued by V5-tagged Neto- and carried through RNAi-mediated knockdown as indicated. The neto-RNAi appears to be more effective than the CUB1RNAi in knocking down V5-tagged Neto- relative to the Tubulin control. Error bars indicate SEM. ***; p<0.001, **; p<0.005, *; p<0.05, ns; p>0.05. Scale bars: 2 μm.</p

The Francis Crick Institute

Representing Roomates' Preferences with Symmetric Utilities

Author: José Álvaro Rodrigues Neto
Publication venue
Publication date
Field of study

In the context of the stable roommates problem, it is shown that acyclicity of preferences is equivalent to the existence of symmetric utility functions, i.e. the utility of agent i when matched with j is the same as j 's utility when matched with i .

Research Papers in Economics

Physiology and morphology defects at neto-β mutant NMJs.

Author: Oghomwen Igiesuorobo (696508)
Mihaela Serpe (696510)
Qi Wang (22418)
Cathy I. Ramos (696509)
Publication venue
Publication date: 2015
Field of study

(A–F) Electrophysiological recordings of control (precise excision) and neto-β alleles of third instar animals. Representative traces of mEJPs and EJPs at 0.8 mM Ca2+ are shown in (A) and (D), respectively, and the results are summarized in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005191#pgen.1005191.s008" target="_blank">S1 Table</a>. The number of NMJs examined is indicated in each bar. The mEJPs amplitude (B) and frequency (C) were reduced for both neto-β alleles. The EJPs amplitude was reduced in netoβshort compared to controls, but was normal at netoβnull NMJs (E). Both alleles had increased quantal content, indicating a presynaptic compensatory response (F). The muscle resting potential and the input resistance were not affected. (G-H) Representative confocal images at NMJ4 and NMJ6/7 (segment A3) in third instar larvae of indicated genotypes labeled with HRP. The neto-β mutant NMJs have significantly fewer and larger boutons relative to the muscle area (quantified in I-J). In particular, the volume of distal boutons increases 2.4 and respectively 2.6 fold at netoβshort and netoβnull NMJs. Error bars indicate SEM. ***; p<0.001, **; p<0.005, *; p<0.05, ns; p>0.05. Scale bars: 20 μm, 2 μm in details.</p

The Francis Crick Institute

Prodomain processing affects Neto-mediated iGluR clustering.

Author: Young-Jun Kim (696507)
Oghomwen Igiesuorobo (696508)
Bing Zhang (129)
Hong Bao (69045)
Mihaela Serpe (696510)
Cathy I. Ramos (696509)
Publication venue
Publication date: 2015
Field of study

(A-B) Confocal images of NMJ4 (A) and bouton details (B) in larvae of indicated genotypes labeled for Brp (green), GluRIIC (red), and Neto (blue). Similar to neto transgenes, CA-neto induced dose-dependent gain-of-function NMJ phenotypes. In contrast, PM-neto transgenes severely disrupted the NMJ morphology and the synaptic contacts. (C) Confocal images of NMJ4 labeled for GluRIIA (green), GluRIIB (red), and HRP (blue) uncovered a drastic reduction of GluRIIA synaptic signals at PM-Neto rescued NMJs. (D) Western blot comparison of Neto expression levels in muscle extracts from third instar larvae rescued with: (i) untagged Neto (lane 2, neto36;G14>neto-A3); (ii) CA-Neto-GFP, normal (lane 3) and high (lane 4); and (iii) PM-Neto-GFP, normal (lane 5) and high (lane 6). (*) uncleaved proteins. (E-F) Quantification of various synaptic signals at neto null NMJs rescued with normal levels of CA- and PM-Neto. (G) Bouton numbers were severely reduced at PM-Neto rescued NMJs. The numbers of NMJs examined are indicated in each bar. Genotypes: CA-Neto normal (neto36;G14>CA-neto-GFP-N4 at 18°C); CA-Neto high (neto36;G14>CA-neto-GFP-N4 at 25°C); PM-Neto normal (neto36;G14>PM-neto-GFP-D2 at 25°C); PM-Neto high (neto36;G14>PM-neto-GFP-D1 at 25°C). Error bars indicate SEM. *; p<0.001, **; p<0.01. Bars: 10 μm, 1 μm in details.</p

The Francis Crick Institute

Listroscelis cyanotibiatus Mendes & Silva-Neto 2023, sp. nov.

Neto-β, but not Neto-α, restores PAK recruitment and mEJP amplitude at <i>neto</i><sup><i>null</i></sup> NMJs.

Parentocirrus brasiliensis Paiva & Silva-Neto 2004, sp. n.

FIGURE 9 in Morphology and morphogenesis of Strongylidium pseudocrassum Wang and Nie, 1935, with redefinition of Strongylidium Sterki, 1878 (Protista: Ciliophora: Stichotrichia)

Poemas atribuidos a Silva

Megatympanon austroraptorum Mendes, Sobral & Silva-Neto 2023, sp. nov.

iGluRs synaptic accumulation is perturbed at <i>neto-β</i> mutant NMJs.

Representing Roomates' Preferences with Symmetric Utilities

Physiology and morphology defects at <i>neto-β</i> mutant NMJs.

Prodomain processing affects Neto-mediated iGluR clustering.