102,281 research outputs found
Supporting evidences for potential biomarkers of endometriosis detected in peripheral blood
No full textAbstractIncidence of endometriosis is very high in women in the reproductive age (around 10%). To date, a reliable non-invasive diagnostic test for early diagnosis of endometriosis is not available. In this article we describe the potential value as diagnostic markers for endometriosis of two proteins (serum albumin and complement C3 precursor), previously identified as differentially expressed in women with endometriosis respect to healthy control by 2D gel analysis. A detailed description of the results obtained with this proteomic approach can be found in Signorile and Baldi [1]. ELISAs were performed on a large cohort of endometriosis (n=100) and healthy patients (n=10) to establish the differential expression of the identified proteins. ROC analyses confirmed the statistical significance of the differential expression of these proteins: serum albumin (p=0.028) ad complement C3 precursor (p=0.082). Evaluation of these two proteins, together with the already described Zn-alpha2-glycoprotein [1], could help in the early identification of endometriosis patient
Plant communities of the southern coast of Bari province
No full textLo studio della vegetazione della costa meridionale della provincia di Bari ha permesso di individuare 23 tipologie di comunità vegetali. I syntaxa di maggiore interesse conservazionistico, habitat ai sensi della Direttiva 92/43/EEC, sono rappresentati dalla vegetazione alofila (Crithmo-Limonietum apuli, Arthrocnemion glauci) ed alo-nitrofila (Pegano-Salsoletea) perenne, comunità annuali psammofile (Cakiletum maritimae), stagni temporanei mediterranei (Isoeto-Nanojuncetea), pratelli terofitici (Brachypodietalia distachyi), lembi di prateria perenne (Thero-Brachypodion ramosi) e di vegetazione rupicola (Asplenietea trichomanis
New Insights in Pathogenesis of Endometriosis
No full textEndometriosis is a gynecological disease characterized by the growth of endometrial glands and stroma outside the uterine cavity. The incidence of the disease is very high, there are currently no reliable early diagnostic tests, the therapies are only symptomatic and, consequently, the social impact of endometriosis is very important, also considering the related fertility problems. Despite this, the pathogenesis of endometriosis is still not fully defined. Retrograde menstruation and coelomic metaplasia are currently the most recognized pathogenetic hypotheses. Recent experimental evidences generated by our research group and by others have indicated an alteration of the fine-tuning of the female genital system developmental program during a critical window of time in the fetal life as the pathogenetic event prompting to the development of endometriosis later in life. Goal of this article is to present a revision of the recent literature about the different pathogenetic mechanisms proposed for endometriosis with particular emphasis on the embryologic theory. The possible clinical and pathological implications of these findings will be discussed
Testing the robustness of a time scale algorithm by using simulated optical clock data
No full textOptical clocks have reached an impressive accuracy, surpassing by orders of magnitude that of microwave clocks, so that the optical transitions on which they are based can be considered as ideal candidates for a possible future redefinition of the second. For these reasons, it is fundamental to develop an effective way to generate time scales based on optical clocks. However, optical clocks are still far from matching the robustness, reliability and long times of autonomous operation typical of microwave clocks. Therefore, the usual way to generate a time scale based on an optical clock, is to use it as a frequency steering reference for a master clock [1], typically an Active Hydrogen Maser (AHM). The master clock is usually referred to as a flywheel oscillator, since it allows the generation of the time scale also when the optical clock is unavailable. It is hence important to understand the impact of the unavailability of the optical clock on the performances of the steering algorithm and of the generated time scale. For example, it is fundamental to understand the minimum availability of the optical clock data needed to guarantee a given level of performances. To this aim, we simulated a time scale steered with an optical clock by considering several possible scenarios for the availability of the latter. By modifying the steering algorithm described in [2], we simulated the measurement data of the frequency offset of an AHM with respect to an optical clock, and we used them to steer the AHM for the time scale generation. Then, we tested the algorithm by considering six different scenarios for the availability of the optical clock, spanning from the ideal one (continuous operation of the optical clock), to the worst one (non-uniformly distributed frequency measurements with long unavailability periods). We also considered a realistic scenario where the optical clock is operated for a few hours once a week, with the possibility of a jitter on the day of the week. Finally, we evaluated the performances by considering the phase offset of the steered time scales with respect to UTC, and we compared and discussed the results obtained within the different scenarios. The results prove that the steering algorithm is robust and effective despite its very simple implementation. As expected, the scenarios giving the best and worst performances are the ideal one and those with long unavailability periods, respectively. More interestingly, the realistic scenario, with one measurement per week only, gives results similar to the ideal scenario ones. This is remarkable, as it means that, even with a much smaller effort dedicated to the optical clock, the final performances of the time scale are still close to the optimal case. This project 18SIB05 ROCIT has received funding from the EMPIR programme co-financed by the Participating States and from the European Union’s Horizon 2020 research and innovation programme. References [1] H. Hachisu, F. Nakagawa, Y. Hanado and T. Ido, “Months-long real-time generation of a time scale based on an optical clock,” Scientific Reports, (2018) 8:4243. [2] L. Galleani, G. Signorile, V. Formichella and I. Sesia, “Generating a real-time time scale making full use of the available frequency standards,” Metrologia 2020, in press
Anti-mullerian hormone is expressed by endometriosis tissues and induces cell cycle arrest and apoptosis in endometriosis cells
No full textBackground: The anti-mullerian hormone (AMH) is a member of the transforming growth factor β (TGF-β) superfamily, which is responsible of the regression of the mullerian duct. AMH is expressed in the normal endometrium, where, acting in a paracrine fashion, negatively regulates cellular viability. Our objective was to evaluate the in vitro effects of the treatment with AMH of endometriosic cells. Methods. AMH expression in human endometriosis glands was evaluated by immunohistochemistry. RT-PCR has been used to quantify the expression levels of AMH and AMH RII isoforms, as well as of cytochrome P450 in both endometriosis epithelial and stromal cells Effects of AMH and AMH-cleaved treatment in endometriosis cells were evaluated by flow-cytometry analysis. Finally, it has been evaluated the effect of plasmin-digested AMH on cytochrome P450 activity. Results: AMH and AMH RII isoforms, as well as cytochrome P450, were expressed in both endometriosis epithelial and stromal cells. Treatment of endometriosis stromal and epithelial cell growth with AMH was able to induce a decrease in the percentage of cells in S phase and increase percentage of cells in G1 and G2 phase; coherently, decreased cell viability and increased percentage of cells death fraction was observed. The plasmin-digested AMH was able to suppress most of the cytochrome P450 activity, causing an increase of pre-G1 phase and of apoptosis induction treating with plasmin-digested AMH in both cell lines, most marked in the epithelial cells. Conclusions: The data produced suggest a possible use of AMH as therapeutic agents in endometriosis. © 2014 Signorile et al.; licensee BioMed Central Ltd
- …
