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RNA nucleoside recognition by phthalocyanine-basedmolecularly imprinted polymers
No full textHighly selective molecularly imprinted polymers
(MIPs) having a phthalocyanine-based recognition centre as
receptors for RNA nucleoside were prepared. In particular,
a zinc phthalocyanine peripherally substituted with meth-
acrylic groups was synthesized and utilized as functional
monomer in combination with methacrylic acid (MAA) to
form polymers with the aim to obtain MIPs having
improved binding capacity and selectivity for nucleosides.
Tri-O-acetyladenosine (TOAA) was utilized as model
template in the preparation of the MIPs. The corresponding
non metallated phthalocyanine was also prepared and used
as functional monomer for the preparation of the MIPs in
order to understand the role of the Zinc(II) ion in the
binding capacity of the polymers towards the template
molecule. The MIP prepared by using both the zinc
phthalocyanine and MAA showed higher binding ability
towards TOAA compared to the MIPs prepared using only
MAA or its combination with the non metallated phthalo-
cyanine. The results obtained suggest that the nucleoside is
specifically bound to the polymer through multi-point
interactions involving both the coordination of the nucleo-
side to the metal of the phthalocyanine and hydrogen
bonding/electrostatic interactions with MAA and the modi-
fiers linked to the phthalocyanines. As for the selectivity,
the MIPs exhibited relative high binding affinities for
TOAA while they did not show any binding capacity for
the other RNA nucleosides
Phthalocyanine-based molecularly imprinted polymers as artificial receptors for DNA and RNA bases.
No full textMolecularly imprinted polymers for solid-phase extraction of 1-methyladenosinefrom human urine
No full textA highly selectivemolecularly imprinted polymer (MIP) for 1-methyladenosine (1-MA), an urinarymod-
ified nucleoside used as cancer marker, was prepared and its use as solid-phase extraction (SPE) sorbent
material was demonstrated. TheMIP was prepared by a very simple procedure usingmethacrylic acid as
functional monomer and a mixture acetonitrile/water (4/1, v/v) as porogen, overcoming in this way the
problems usually related to the imprinting of biological polar compounds. The MIP was tested in batch
experiments in order to evaluate its binding properties and then used as SPE sorbent for the selective
clean-up and pre-concentration of 1-MA. The extraction protocol was successfully applied to the direct
extractionof 1-MAfromspikedhumanurine indicating that theMIP allowed 1-MAto be pre-concentrated
while simultaneously interfering compounds were removed from the matrix
Water-compatible molecularly imprinted polymer for solid phase extraction of 1-methyladenosine as biochemical marker
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Development in the synthesis of molecularly imprinted polymers with different functional monomers
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Development of a molecularly imprinted ploymer for determination of 3-nitrotyrosine used as potential oxidative stress marker
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