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    The phylogenetic relationships of Triturus italicus

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    Several studies attempted to reconstruct the phylogenetic relationships among the species of Tritums by a number of data sets from morphology, courtship behaviour, reproductive interactions, cytogenetics and molecules. The present paper critically reviews those studies with regard to the phylogenetic affinities of T. italicus (Peracca, 1898). Most studies locate T. italicus within the radiation of the small-sized newts (subgenus Palaeotriton), but are in conflict about its sister-group, whether T. vulgaris or T. boscai. Recent studies on mtDNA sequences suggest that Triturus is paraphyletic and several other genera should be included within the genus, with T. italicus being an outgroup of all the other species and genera. It is concluded that, at present, little can be stated about the relationship of T. italicus and that new data should be collected

    Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

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    The mucins secreted by the Brunner's glands and the duodenal goblet cells of the Guinea-pig and the house mouse were compared by conventional and FITC-conjugated lectin histochemistry. Methylation/saponification and sialidase digestion were performed prior to lectin binding to detect the residues subterminal to sulfated groups and sialic acid, respectively. In the Guinea-pig the Brunner's glands produce class-III stable sulfosialomucins. Sialic acid is mostly 2,6-linked to galactose or to N-acetylgalactosamine and is in part O-acetylated in C7, C8, and C9. Sulfated groups are probably linked to sialic acid and N-acetylgalactosamine. Terminal residuals of N-acetylglucosamine, galactose, N-acetylgalactosamine and fucose linked in alpha1,2, alpha1,3, and alpha1,4 are also present. Duodenal goblet cells of the Guinea-pig present a lower number of residuals in respect to the Brunner's glandular ones, with sialic acid and N-acetylgalactosamine subterminal to sulfated groups. In the house mouse the Brunner's glands produce class-III stable neutral mucins, binding to same lectins as in the Guinea-pig except for those specific to sialic acid. A diversity of fucosylated residuals higher than in the Guinea-pig is observed. The mouse duodenal goblet cells lack stable class-III mucins, have little sialic acid and present a lower number of residuals in respect to the correspondent Brunner's glands. Regulation of the acidic intestinal microenvironment, prevention of pathologies and hosting of microflora can explain the observed results and the differences observed between the two rodents
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