1,721,022 research outputs found
Cesium chloride or column preparation? An electron microscopical view of plasmid preparations
No full textSchleef M, Heimann P. Cesium chloride or column preparation? An electron microscopical view of plasmid preparations. Biotechniques. 1993;14(4):544
Plasmid topology - An important factor influencing the performance of DNA drug delivery
No full textSchleef M, Voß C, Schmidt T, Flaschel E. Plasmid topology - An important factor influencing the performance of DNA drug delivery. JOURNAL OF LIPOSOME RESEARCH. 2003;13(1):82-83
CHROMOSOMAL LOCALIZATION AND GENOMIC CLONING OF THE MOUSE ALPHA-TROPOMYOSIN GENE TPM-1
No full textSCHLEEF M, WERNER K, SATZGER U, KAUPMANN K, Jockusch H. CHROMOSOMAL LOCALIZATION AND GENOMIC CLONING OF THE MOUSE ALPHA-TROPOMYOSIN GENE TPM-1. GENOMICS. 1993;17(2):519-521
Plasmid vector stability in production, application and storage
No full textSchleef M, Voß C, Schmidt T, Flaschel E. Plasmid vector stability in production, application and storage. JOURNAL OF GENE MEDICINE. 2003;5(3):S26-S26
Minicircle DNA - how small is "mini"?
No full textRischmueller A, Deglon N, Schleef M, Anselmetti D. Minicircle DNA - how small is "mini"? Human Gene Therapy. 2013;24(12):A67-A67
Stabilizing ITR elements in AAV transfer plasmids
No full textShankar R, Radukic M, Schäffer N, Schmeer M, Schleef M, Müller K. Stabilizing ITR elements in AAV transfer plasmids. Human Gene Therapy . 2025;36(3-4):E246
Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use
No full textVoß C, Schmidt T, Schleef M, Friehs K, Flaschel E. Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. 2004;79(1):57-62.In this paper the influence of ammonium salt concentration on the production of pharmaceutical grade plasmid DNA from unfed high cell density batch culture on synthetic glycerol media is presented. Ammonium chloride in different concentrations (0 mmol dm(-3), 18.7 mmol dm(-3), 37 mmol dm(-3) and 74 mmol dm(-3) supplemented) was used beside sodium glutamate as nitrogen source. Plasmid DNA concentrations of more than 50 mg dm(-3) were obtained with 37 mmol dm(-3) ammonium. The homogeneity of the DNA produced was confirmed by agarose and capillary gel electrophoresis and found to fulfil the quality requirements set for biopharmaceutical plasmid DNA with more than 90% in the supercoiled form. (C) 2003 Society of Chemical Industry
The gene encoding sarcoplasmic reticulum calcium ATPase-1 (Atp2a1) maps to distal mouse chromosome 7
No full textSchleef M, SimonChazottes D, Lengeling A, et al. The gene encoding sarcoplasmic reticulum calcium ATPase-1 (Atp2a1) maps to distal mouse chromosome 7. MAMMALIAN GENOME. 1996;7(10):788
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