118,748 research outputs found
Identification and detection of Rosellinia necatrix by conventional and real-time Scorpion PCR
No full textSeveral polymerase chain reaction (PCR) primers were designed from the internal transcribed spacer (ITS) regions of the rDNA genes of Rosellinia necatrix to develop a PCR-based identification method. Screening the primers against two isolates of R. necatrix and six other Rosellinia species resulted in the amplification of a single specific product from R. necatrix for most of the primer pairs. Two primer pairs (R2-R8 and R10-R7) confirmed their specificity when tested against 72 isolates of R. necatrix and 93 other fungi from different hosts and geographic areas. The R10 primer was modified to obtain a Scorpion primer for detecting a specific 112 bp amplicon by fluorescence emitted from a fluorophore in a self-probing PCR assay. This assay specifically recognised the target sequence of R. necatrix over a large number of other fungal species. In conventional PCR, with primer pairs R2-R8 and R10-R7, 10-fold dilutions of R. necatrix DNA indicated a detection limit of 10 pg muul(-1) using a single set of primers and 10 fg mul(-1) in nested-PCR. For Scorpion-PCR, the detection limit was 1 pg mul(-1) and 1 fg mul(-1) in nested Scorpion-PCR, i.e. 10 times more sensitive than conventional PCR. A simple and rapid procedure for DNA extraction directly from soil was modified and developed to yield DNA of purity and quality suitable for PCR assays. Combining this protocol with the nested Scorpion-PCR procedure it has been possible to specifically detect R. necatrix from artificially inoculated soils in approximately 6 h
Sardinia. A Mediterranean Crossroad. 12th Annual Mediterranean Studies Congress (Cagliari, 27-30 maggio 2009), RiMe, rivista dell'ISEM-CNR, n.4, giugno 2010
No full textPresentazione [L'italiano lingua utilitaria]
No full textPresentazione del libro: L'italiano lingua utilitari
Rapid and sensitive detection of Rosellinia necatrix in roots and soils by real time Scorpion-PCR
No full textEnseigner et apprendre le jeu modal: question de méthode
No full textLe subjonctif a reçu maintes définitions selon les différents signifiés des phrases où il apparaît. Toutefois, malgré l’extrême hétérogénéité du discours, le système présente une syntaxe cohérente. Cet aspect est généralement admis, mais les approches pédagogiques ne proposent pas un modèle unitaire d’interprétation du mode subjonctif. Elles mettent l’accent sur les formes et sur les moyens de les assimiler dans le bon contexte, mais n’enquêtent pas sur les raisons de leur emploi. L’idée de présenter la psychomécanique du langage à des étudiants universitaires est motivée par le fait que cette théorie condense de multiples effets à travers les régularités des interceptions du temps opératif. Grâce à cet appareil théorique, les apprenants comprennent les deux volets du langage : la complexité de l’effectif et la simplicité du potentiel. La charpente du mode est décrite de façon à ce que les apprenants trouvent un lien entre leur activité psychique et leur production langagière. Le fait de dévoiler l’ « ordre secret » du discours, confirmant ainsi que la langue est systématique, les encourage à rechercher les éléments communs sous-jacents. Pour les aider à travailler de manière inductive, on trace d’abord les lignes de force générales de l’emploi du subjonctif. La méthode pédagogique s’avère ainsi un va-et-vient de la théorie à l’analyse des faits langagiers tirés de la presse, mais aussi d’ouvrages littéraires, la diversité des genres écrits étant importante pour comprendre la simplification actuelle du système « à quatre temps » du subjonctif. La théorie guillaumienne contribue à renforcer l’intuition des apprenants quand elle est pédagogisée pour développer non seulement un savoir grammatical, mais aussi une conscience linguistique, quand elle est inscrite dans la méthode de « linguistique-didactique » pour transmettre des compétences et affiner une certaine sensibilité à l’égard du binôme langue-culture
Effects of Flywheel Strength Training on the Running Economy of Recreational Endurance Runners
No full textFesta, L, Tarperi, C, Skroce, K, Boccia, G, Lippi, G, La Torre, A, and Schena, F. Effects of flywheel strength training on the running economy of recreational endurance runners. J Strength Cond Res 33(3): 684-690, 2019-Running economy (RE) has been defined as the most important determining factor in endurance performance in both elite and recreational runners. The purpose of this study was to evaluate the effect of flywheel strength training (FST) and high-intensity training (HIT) protocols on RE and strength parameters in a group of recreational runners. Twenty-nine recreational runners were recruited to take part in the study and were randomly assigned to FST (n = 9; 44.5 ± 6.0 years; V[Combining Dot Above]O2max 48.8 ± 5.2 ml·min·kg), HIT (n = 9; 42.2 ± 8.6 years; V[Combining Dot Above]O2max 50.3 ± 3.7 ml·min·kg), or low-intensity training (LIT) (n = 11; 45.4 ± 8.0 years; V[Combining Dot Above]O2max 50.2 ± 6.8 ml min kg) groups. Before and after 8 weeks of an experimental period, maximal oxygen uptake (V[Combining Dot Above]O2max), ventilatory thresholds (VTs), maximal dynamic force (1 repetition maximum [1RM]), and anthropometric data were evaluated. The FST group showed significant increases (p < 0.05) in 1RM and RE. No differences were found in the other groups. Significative changes are found for all groups on average speed on 2 and 10 km (p < 0.05). Anthropometric data were unchanged after the training period. The results of this study indicate that in recreational runners, FST seems able to obtain improvements in RE and neuromuscular adaptation
Resoconto di un 'divertissement' grammaticale sul congiuntivo in italiano e nel dialetto di Bormio
No full textLa scoperta della cronogenesi mostra l'affidabilità del metodo di Gustave Guillaume, fondato sul tempo operativo. In maniera semplice e complessa al tempo stesso, la sua «linguistique de position» spiega i modi secondo intercettazioni del processo di formazione dell’immagine-tempo da parte del soggetto locutore che intende situare un’azione nel tempo. Nella comparazione dei dati italiano standard/varietà bormina è stato stabilito un rapporto tra le cosiddette sollecitazioni mentali «silenziose» delle forme modali del congiuntivo e la loro espressione, mettendo in relazione critica l'approccio semasiologico tradizionale e quello onomasiologico del metodo psicomeccanico
Increasing relevance of donor-specific antibodies in antibody-mediated rejection.
No full textOne of the major concerns in organ transplantation is the early detection of humoral rejection, through improved diagnostic and prognostic biomarkers. Long-term survival of renal allografts is significantly lower in recipients developing donor-specific anti-HLA antibodies (DSAs) either pretransplant or posttransplant. Patients can form antibodies following blood transfusions, pregnancies or previous transplants. DSAs can lead to endothelial damage through complement-dependent or independent pathways. Universal testing of kidney transplant patients and careful monitoring of graft function if DSAs are detected are recommended. Since there are different techniques to detect DSAs presence and serum levels, nephrologists have to face challenges in their interpretation due to variable sensitivity and specificity. Moreover, other biomarkers of rejection (T-cell reactivity, gene expression pattern modulation, early features of immunological damage in protocol renal biopsies) may be adopted together with DSAs detection tools, thus providing a global approach to the issue. To date, however, there are no well-defined strategies of intervention in cases of humoral graft damage. Future resolution of both interpretative and therapeutic concerns will make DSAs monitoring a very effective way to predict incoming immunological events. Therefore, an operative protocol for DSAs detection in renal recipients has been illustrated
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