1,721,131 research outputs found
development of a nested-PCR protocol for detection of olive-infecting viruses in crude extracts
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Attempts to Reduce the Systemic Spread of Xylella fastidiosa in Olive Trees by Pruning
Full text linkXylella fastidiosa subsp. pauca (Xfp) is the plant pathogenic bacterium causing the epidemic of olive quick decline syndrome decimating olive trees in the Apulia region (southern Italy). The lack of any effective therapeutic application for the control of this pathogen and its categorization as a regulated quarantine pathogen in many countries worldwide, impose mandatory eradication and containment measures. Based on current EU legislation, containment measures apply in those areas where the bacterium is widely established, such as in the Apulia region, and thus containment strategies to mitigate and cope with the infections are needed. We set up a field trial to assess if pruning interventions could limit and/or recover Xfp-infected trees by reducing the systemic spread of the bacterium and the severity of the desiccation phenomena typically compromising the crown of the highly susceptible cultivars, e.g., cv. Cellina di Nardò. Trees subjected either to major or light pruning interventions, including the removal of all the symptomatic branches, did not demonstrate a reduced bacterial colonization or development of symptoms. After two years of targeted pruning interventions, no significant amelioration of the sanitary status of the infected olive trees was recorded, suggesting that the sole application of these interventions is not effective to counteract the impact of the bacterium in the susceptible olive trees
Presence of viruses in croatian olive groves
Full text linkDuring the years 2005 and 2006, testing of the olive plant material on the presence of olive viruses was conducted for the first time in Croatia. The samples for testing were collected from twenty five locations in commercial olive orchards, from traditional olive growing areas. All samples were teseted on the presence of eight virus types: ArMV, CMV, CLRV, OLV-1, OLV-2, OLRV, OLYaV and SLRV.
The results of analyses conducted by using the RT-PCR method, confirmed the presence of viruses in 25% of samples, and four virus types were confirmed: OLYaV - Olive leaf yellowing-associated virus, CMV - Cucumber mosaic virus, OLV-2 - Olive latent virus 2 and SLRV – Strawberry latent ringspot virus. Presence of CMV was confirmed on Istarska bjelica and Lastovka samples, SLRV was confirmed on Levantinka and Oblica samples, OLV-2 was confirmed on Lastovka samples, OLYaV was confirmed on Bjelica, Istarska bjelica, Lastovka and Levantinka samples. Samples of Buža, Perišićeva mastrinka and Rosulja cultivars were free from tested viruses
Tematica I - Manuale per l'identificazione e il riordino del patrimonio varietale di olivo
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Coding-Complete Genome Sequence of a Black Queen Cell Virus Isolate from Honey Bees (Apis mellifera) in Italy
Full text linkIn this study, we documented the complete coding genome sequence of a Black queen cell virus (BQCV) isolate from honey bees in Italy. This genome sequence illustrates a high similarity with other BQCV isolates reported worldwide and could provide insights into BQCV genome phylogeny and divergence
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