1,721,024 research outputs found
Cytotoxicity and apoptosis induction by e-cigarette fluids in human gingival fibroblasts
No full textElectronic cigarettes (e-cigarettes) are generally acknowledged as a safer alternative to the use of combusted tobacco products. Nevertheless, there are increasing conflicting claims concerning the effect of these novel industrial products on the health of e-cigarettes users. The aim of this work was to investigate the effects of the liquids of e-cigarettes on human gingival fibroblasts (HGFs) and to compare the effects of nicotine-containing fluid to the fluid itself. HGFs were treated with different concentrations (0-5 mg/mL) of fluids of e-cigarettes for different times (0-72 h) and cytotoxicity was analyzed by MTT assay. Fluids were administered also after being vaped (e.g., warmed into the cartomizer). Apoptosis occurrence and Bax expression were evaluated by flow cytometry; ROS production was analyzed by fluorescence optical microscopy. Both nicotine-containing and nicotine-free fluids induced an increased ROS production after 24 h, along with an increased Bax expression, followed by apoptosis occurrence after 48 h of exposure. The cytotoxicity exerted on HGFs by e-cigarettes fluids is not entirely ascribable to nicotine. Since the e-cigarettes are advertised as a safer alternative to traditional ones, especially for the possibility of "smoking" nicotine-free fluids, further studies are necessary to clarify the mechanism involved in the occurrence of cytotoxicity exerted by such compounds. Our results suggest a role for e-cigarette fluids in the pathogenesis of oral diseases, such as periodontitis
Nutlins and Ionizing Radiation in Cancer Therapy
No full textRadioresistance stands as a fundamental barrier that limits the effectiveness of radiotherapy in cancer treatment. Recent evidences suggest that radioresistance is due to tumour repopulation and involves several signalling pathways, including p53/MDM2 interaction. Ionizing radiation induces p53-dependent MDM2 gene transcription that, in turn, inhibits p53 transcriptional activity, favouring its nuclear export and stimulating its degradation. In light of the observation that in many human tumours the inadequate function of p53 is the result of MDM2 over-expression, several authors have considered as an attractive therapeutic strategy to activate p53 signalling in tumours by inhibiting MDM2 activities or p53/MDM2 interaction. We retain that, by preventing the interaction p53/MDM2 with Nutlin, a small molecule that binds at the interface between these two proteins, the effectiveness of ionizing radiation treatment could be improved. Promising results have recently emerged from in vitro studies performed on laryngeal, prostate and lung cancer cell lines treated with Nutlin in combination with ionizing radiation. Based on these findings, we believe that the combined approach Nutlin/ionizing radiation should be further investigated for efficacy on both solid tumours and lymphoproliferative disorders as well as for side effects on normal cells and tissues. Therefore, the purpose of this review is to report the first results obtained by using Nutlins alone or in combination with other therapeutic agents on primary tumour cells, in vitro cell lines or tumour xenografts and to present the most recent advances in the understanding of the molecular mechanisms underlining ionizing radiation cytotoxicity and resistance
A flow cytometric analysis of mitochondrial cell death in HGFs/S. mitis co-culture model
No full textTRAIL induces cAMP-response element binding (CREB) protein phosphorylation in Jurkat T leukaemic cells
No full textValutazione comparativa degli effetti del trattamento con agonisti ed antagonisti del GnRH sull’apoptosi delle cellule della granulosa del follicolo preovulatorio umano
No full textEffects of a new nanocomposite system on Human Gingival Fibroblasts/Streptococcus mitis co-culture
No full textInvolvement of the TRAIL-TRAIL/Receptors (TRAIL-R) system in the model of gestational diabetes (GD)
No full textRisposta biologica di un modello di co-coltura Streptococchi/Fibroblasti gengivali umani al 2-idrossietil- metacrilato in presenza di saliva
No full textEffetti di Biomateriali sul modello di co-coltura Streptococchi orali/ fibroblasti gengivali umani
No full textMorphological and molecular modifications during erythroid differentiation: role for CREB nuclear transcription factor
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