1,721,008 research outputs found
“Signalling molecules of gram-negative bacteria investigated by LC-ESI-LTQ-FTMS”
No full textComunicazione ORALE
Identificazione di molecole mediatrici del Quorum Sensing in batteri Gram-Negativi mediante cromatografia liquida e spettrometria di massa con trappola ionica ciclotronica in trasformata di Fourier (LC-ESI-FTICR-MS)
No full textComunicazione ORALE
Identificazione dei biomarker usati dai batteri Gram-negativi per la comunicazione intercellulare mediante cromatografia liquida e spettrometria di massa con trappola ionica ciclotronica in trasformata di Fourier (LC-ESI-FTICR-MS)
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S-Adenosilmetionina e derivati nel percorso metabolico dei batteri: studio preliminare LC-ESI-FTICRMS per lo sviluppo di farmaci antimicrobici
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Identificazione di molecole segnale mediante cromatografia liquida e spettrometria di massa in trasformata di Fourier (LC-ESI-FTMS) ed implicazioni del quorum sensing in batteri gram-negativi coinvolti nei processi di biorisanamento ambientale.
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Analisi dei metaboliti della caffeina in campioni di urina mediante LC-ESI-FTICR-MS e spettrometria di massa tandem in trappola ionica lineare (LTQ)
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Separazione cromatografica (LC) e caratterizzazione ESI-LTQ-FTMS di nido-caborani: rilascio facile di idrogeno molecolare
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Accurate mass analysis of N-acyl-homoserine-lactones and cognate lactone-opened compounds in bacterial isolates of Pseudomonas aeruginosa PAO1 by LC-ESI-LTQ-FTICR-MS
No full textN-acyl-homoserine-lactones (AHSLs) are widely conserved signal molecules present in quorum sensing systems of Gramnegative
bacteria such as Pseudomonas aeruginosa. We present here the results obtained with a hybrid linear trap/Fourier
transform ion cyclotron resonance (LTQ-FTICR) mass spectrometer used to investigate the occurrence of AHSLs and cognate
N-acyl-homoserines (AHSs) in bacterial isolates of P. aeruginosa (strain PAO1). Two hydrolysed AHSs were found in significant
amounts, most likely formed through the lactone opening of N-3-oxo-decanoyl-L-homoserine-lactone (3OC10-HSL) and
N-3-oxo-dodecanoyl-L-homoserine-lactone (3OC12-HSL). Structure elucidation of these ring-opened molecules, i.e. N-3-oxodecanoyl-
L-homoserine (3OC10-HS), and N-3-oxo-dodecanoyl-L-homoserine (3OC12-HS), which are not detected by bacterial
biosensors, was performed by high-resolution and accurate mass measurements upon liquid chromatography (LC) and
confirmed by tandem MS in the LTQ analyser. Assignment of chemical formula, with mass spectra in the form of [M + H]+, was
significantly expedited by extracted ion chromatograms (XICs) because the number of potentially plausible formulae for each
protonated signalling molecule was considerably reduced a priori by the LC behaviour, the high mass measurement accuracy
available in FTICR mass spectra and the isotopic patterns. At least two concentration levels were observed in spent culture
supernatants of P. aeruginosa: compounds at a relatively high content (5–15 μM) that is C4-HSL, 3OC10-HS, and 3OC12-HS and
those occurring at a lower content (<0.2 μM) that is C6-HSL and C8-HSL. The implications of this work extend to a great variety
of Gram-negative bacteria
“FRAGMENTATION STUDY OF CAFFEINE AND ITS XANTHINE METABOLITES IN URINE SAMPLES BY LC-ESI-MS AND TANDEM MS”
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