1,721,034 research outputs found
Small RNA isolation from tissues of grapevine and woody plants
No full textA protocol is described to purify small (s)RNA molecules from tissues of grapevine and other woody plants. The protocol has been specifically developed to analyze sRNA populations by high-throughput sequencing. It has been widely used on species of the genera Prunus and Vitis particularly rich in polyphenols and other enzyme-inhibiting compounds. The high quality of the sRNAs extracted from leaf or phloem tissues makes them suitable for all molecular biology reactions, in particular for next-generation sequencing library preparation
BIONFORMATIC ANALYSIS OF NGS LIBRARIES: A MODERN TOOL FOR DISCOVERY AND CHARACTERIZATION
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Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.)
No full textA quince tree showing severe symptoms of a
previously undescribed viral disease occurring in northern
Apulia (Italy) was analysed using high-throughput
sequencing of small RNA libraries, leading to the identification
of a new strain of apple green crinkle associated
virus (isolate AGCaV-CYD) showing peculiar traits. RTPCR
with specific primers detected AGCaV-CYD in consistent
association with symptoms in the surveyed orchards.
Molecular characterization of the reconstructed
genome, together with phylogenetic analysis, showed it to
be closely related to an AGCaV strain causing green
crinkle disease in apple (AGCaV-AUR) and divergent from
the type strain of apple stem pitting virus (ASPV-PA66)
Molecular characterisation of a novel gemycircularvirus associated with olive trees in Italy
No full textA new gemycircularvirus sequence was obtained from total DNAs extracted from olive leaves and twigs tissues. Its complete genome consists of a single-stranded circular DNA of 2,145 nt, whose sequence was obtained by rolling circle amplification (RCA). Genome analysis identified three open reading frames, sharing homologies with the coat and replication-associated proteins, these latter in the anti-parallel strand, of known gemycircularvirus species. Search for homologies showed that the circular ssDNA sequence is distantly related to other gemycircularviruses thus originating from a new virus species, for which the name olive-associated gemycircularvirus 1 (OaGV1) is proposed. A survey in two different olive-growing areas of the Apulian region (Southern Italy), showed a limited distribution of OaGV1
Le opportunità del Next Generation Sequencing nella ricerca e nella diagnostica fitovirologica.
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COMPARATIVE PROFILING OF SMALL RNA POPULATIONS IN VIRUS-INFECTED AND HEALTHY GRAPEVINE PLANTS
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