112 research outputs found
Letter from Frank G. Muench, May 25, 1942
No full textAnnotated form letter from Frank G. Muench regarding the fact that Lincoln Christian Center in Sacramento, California helped their Japanese friends move safely to Walerga camp [= Sacramento Assembly Center] about 10 miles from Sacramento.The Japanese American Archival Collection documents the people, places, and daily life of Japanese Americans, primarily those who lived in the once thriving community of pre-war Florin in the Sacramento region, as well as the conditions in American incarceration camps during World War II. The approximately 7,000 original items include personal and official letters, photographs, diaries, arts and crafts, newsletters, textiles, camps artifacts, yearbooks and other publications
Characterization of the flexibility of the peripheral stalk of prokaryotic rotary A-ATPases by atomistic simulations
No full textRotary ATPases are involved in numerous physiological processes, with the three distinct types (F/A/V-ATPases) sharing functional properties and structural features. The basic mechanism involves the counter rotation of two motors, a soluble ATP hydrolyzing/synthesizing domain and a membrane-embedded ion pump connected through a central rotor axle and a stator complex. Within the A/V-ATPase family conformational flexibility of the EG stators has been shown to accommodate catalytic cycling and is considered to be important to function. For the A-ATPase three EG structures have been reported, thought to represent conformational states of the stator during different stages of rotary catalysis. Here we use long, detailed atomistic simulations to show that those structures are conformers explored through thermal fluctuations, but do not represent highly populated states of the EG stator in solution. We show that the coiled coil tail domain has a high persistence length (∼100 nm), but retains the ability to adapt to different conformational states through the presence of two hinge regions. Moreover, the stator network of the related V-ATPase has been suggested to adapt to subunit interactions in the collar region in addition to the nucleotide occupancy of the catalytic domain. The MD simulations reported here, reinforce this observation showing that the EG stators have enough flexibility to adapt to significantly different structural re-arrangements and accommodate structural changes in the catalytic domain whilst resisting the large torque generated by catalytic cycling. These results are important to understand the role the stators play in the rotary-ATPase mechanism. Proteins 2016; 84:1203–1212. © 2016 The Authors. Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc
Understanding the apparent stator-rotor connections in the rotary ATPase family using coarse-grained computer modeling
No full textAdvances in structural biology, such as cryo-electron microscopy (cryo-EM) have allowed for a number of sophisticated protein complexes to be characterized. However, often only a static snapshot of a protein complex is visualized despite the fact that conformational change is frequently inherent to biological function, as is the case for molecular motors. Computer simulations provide valuable insights into the different conformations available to a particular system that are not accessible using conventional structural techniques. For larger proteins and protein complexes, where a fully atomistic description would be computationally prohibitive, coarse-grained simulation techniques such as Elastic Network Modeling (ENM) are often employed, whereby each atom or group of atoms is linked by a set of springs whose properties can be customized according to the system of interest. Here we compare ENM with a recently proposed continuum model known as Fluctuating Finite Element Analysis (FFEA), which represents the biomolecule as a viscoelastic solid subject to thermal fluctuations. These two complementary computational techniques are used to answer a critical question in the rotary ATPase family; implicit within these motors is the need for a rotor axle and proton pump to rotate freely of the motor domain and stator structures. However, current single particle cryo-EM reconstructions have shown an apparent connection between the stators and rotor axle or pump region, hindering rotation. Both modeling approaches show a possible role for this connection and how it would significantly constrain the mobility of the rotary ATPase family
-ATPase
No full textThe V-ATPase is a membrane-bound protein complex which pumps protons across the membrane to generate a large proton motive force through the coupling of an ATP-driven 3-stroke rotary motor (V1) to a multistroke proton pump (Vo). This is done with near 100% efficiency, which is achieved in part by flexibility within the central rotor axle and stator connections, allowing the system to flex to minimise the free energy loss of conformational changes during catalysis. We have used electron microscopy to reveal distinctive bending along the V-ATPase complex, leading to angular displacement of the V1 domain relative to the Vo domain to a maximum of ∼30°. This has been complemented by elastic network normal mode analysis that shows both flexing and twisting with the compliance being located in the rotor axle, stator filaments, or both. This study provides direct evidence of flexibility within the V-ATPase and by implication in related rotary ATPases, a feature predicted to be important for regulation and their high energetic efficiencies. © 2013 Song et al
Learning to Predict Motion from Raw 3D Object Detections
No full textWe show how to design a motion prediction algorithm that works with 3D object detections and map locations. In particular, we obtain object id’s – even though the training data does not contain any object id’s – across multiple time-steps into the future by propagating a Gaussian Mixture of likely object (e.g., vehicle) locations through time.We validate our approach on the nuScenes dataset. First, we find that a motion prediction algorithm without tracking id’s performs as well as motion prediction algorithm with tracking id’s in the training data. Second, the 3D labels of an on-board perception system are inferior (e.g., loss of detections, positional uncertainty) to those generated by offline labelling (automatic labelling pipeline, manual labelling). Even so, we find that a moderate increase in the size of the training data offsets the deterioration in prediction performance (with no additional offline labelling).Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Intelligent Vehicle
Animations: The Photospheric Footpoints of Solar Coronal Hole Jets
No full textThis repository provides the individual animations of the SDO/AIA and HMI magnetograms of Jets 1, 2, 3, corresponding to jets 14, 32, 35 in Table 1 of the accepted manuscript, "The Photospheric Footpoints of Solar Coronal Hole Jets".
jet1-aia193.mp4: AIA 193A movie of Jet 1
jet1-hmimag.mp4: HMI magnetograms movie of Jet 1
jet1-aia193-hmi-contour.mp4: AIA 193A with HMI contours movie of Jet 1
fig1anim.mp4: Concatenation of Jet 1 movies shown in Figure 1 of the accepted manuscript.
jet2-aia193.mp4: AIA 193A movie of Jet 2
jet2-hmimag.mp4: HMI magnetograms movie of Jet 2
jet2-aia193-hmi-contour.mp4: AIA 193A with HMI contours movie of Jet 2
fig4anim.mp4: Concatenation of Jet 2 movies shown in Figure 4 of the accepted manuscript.
jet3-aia193.mp4: AIA 193A movie of Jet 3
jet3-aia304.mp4: AIA 304A movie of Jet 3
jet3-hmimag.mp4: HMI magnetograms movie of Jet 3
jet3-aia193-hmi-contour.mp4: AIA 193A with HMI contours movie of Jet 3
fig6anim.mp4: Concatenation of Jet 3 movies shown in Figure 6 of the accepted manuscript.This work has been supported by NSF (AGS-1159353) and NASA (project 80NSSC18K0716). AIA and HMI data are courtesy of NASA/SDO and the AIA and HMI science teams. The author would like to thank P.R. Young for the analysis of the EIS data. This research has made use of NASA's Astrophysics Data System
Principal induction: principals' perceptions, 1998
No full textThis study served a dual purpose: (1) to assess thedegree to which principals received the practices that current literature describes as being advantageous in an induction program for newly appointed principals; and (2) to recommend a literature-based, principal influenced principal induction program for the DeKalb County School System. Current literature in the field provides sparse information on needs of newly hired principals during their infant years and strategies used in school districts to induct principals. Through this study's description of induction experiences stated as advantageous to an induction program, educators and school systems can gain insight into induction practices that could assist principals during their first three years of school leadership. The subjects of the study were 155 principals active during the 1996-97 school year in the metropolitan Atlanta RESA. In this quantitative investigation, data for the study were obtained through a 142-item questionnaire that focused on induction practices research indicated were important for newly hired principals. These induction practices were clustered into seven construct areas: Personal Aspects of Induction, Time Periods of Induction, Job-Specific Content, Profession-Specific Content, Induction Focus, Mentoring, and Sequencing Induction Activities. Mean scores indicated that principals rated all seven constructs at an inadequate to less than adequate level of received induction. The respondents stated each induction construct was important to include in an induction program for newly hired principals. The ANOVA test revealed significant differences in the principals' perception of the induction they received with respect to Personal Aspects of Induction, Job-Specific Content, Profession-Specific Content, and Induction Focus practices. A difference was also found in the category pertinent to years of current principalship experience. The findings indicated that the induction practices considered important by current literature and principals were not being received during the first three years of principalship. Recommendations for implementing or improving a formal school district induction program should follow the seven construct areas, with a special focus on formalization of an induction plan, planning thorough school and community orientation, and mentoring
Composable Q- functions for pedestrian car interactions
No full textWe propose a novel algorithm that predicts the interaction of pedestrians with cars within a Markov Decision Process framework. It leverages the fact that Q-functions may be composed in the maximum-entropy framework, thus the solutions of two sub-tasks may be combined to approximate the full interaction problem. Sub-task one is the interaction-free navigation of a pedestrian in an urban environment and sub-task two is the interaction with an approaching car (deceleration, waiting etc.) without accounting for the environmental context (e.g. street layout). We propose a regularization scheme motivated by the soft-Bellman-equations and illustrate its necessity. We then analyze the properties of the algorithm in detail with a toy model. We find that as long as the interaction-free sub-task is modelled well with a Q-function, we can learn a representation of the interaction between a pedestrian and a car
The circulation of Prince William Sound
No full textThere are no author-identified significant results in this report
Graphene electrically tuneable third harmonic generation
No full textElectrical control of the nonlinear optical response enables applications such as gate-tunable switches and frequency converters. Graphene displays strong-light matter interaction and electrically and broadband tunable third order nonlinear susceptibility. We show that the third harmonic generation efficiency in graphene can be tuned by over two orders of magnitude by controlling the Fermi energy and the incident photon energy
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