59 research outputs found
Distribution, resources, features of biology, and the first results of specialized fishery of shrimp <i>Pandalus goniurus</i> in the Tatar Strait
No full textShrimp Pandalus goniurus is poorly known in the Tatar Strait. Its distribution, state of stock, and features of biology are described on the data of 7 trawl surveys conducted by RV Buhoro in the western Tatar Strait in April-June 2009-2010 (227 trawls) and September-October 2011-2014 (589 trawls) and commercial catches obtained by fishing vessel Sovetskoye in May 2014 (50 trawls) and April 2015 (59 trawls). Horizontal opening of the scientific and commercial trawls was 16 and 35 m, respectively. About 14,000 individuals of the shrimp were subjected to biological analysis. P. goniurus was distributed along the whole continental coast of the strait northward from 47°20′ N and in spring concentrated at the depth from 50 to 120 m, rarely to 270-300 m, with single findings down to 340 m. Its stock fluctuated from 16 to 70 thousand t, on average 45,000 t (50-70 % within the territorial waters); possible annual catch was estimated as 5,000 t. Commercial fishery of this species was started in 2014 (mean CPUE 1.91 t/hour), the number of fishing vessels increased to 3 in 2015 (mean CPUE 3.26 t/hour, total landing 1160 t). The fishery is distinguished by easy hydroacoustic search of aggregations, long-time fishing within limited areas, high catches, including the catches in nighttime, and low bycatch. The highest concentration of P. goniurus was registered in April 2015: CPUE 11.86 t/hour, density 73.2 t/km2 or 10 ind./m2. Size-weight parameters of P. goniurus in the Tatar Strait were the best for all its populations: their average values in commercial catches in 2014/2015 were: carapace length 20.4/22.4 mm, individual weight 5.7/7.4 g or 165/116 ind./kg. Its larvae hatching was observed in April-May, mass post-hatching molt of females was supposedly in June-July and breeding molt and spawning - in September-October. First results of the fishery are analyzed, its prospects are estimated, and measures for its regulation are proposed
Изучение коллекционного материала озимой твердой пшеницы на наличие гена устойчивости к септориозу Stb2
No full textIn order to obtain high yields of winter durum wheat, it is necessary not only to use advanced agricultural technologies, but also to sow varieties resistant to major diseases. One of these diseases that cause great harm to grain crops is Septoria leaf blotch. The current paper has presented the study results of collection material of Russian and foreign winter durum wheat according to the efficiency of the Septoria leaf blotch resistance gene Stb2 in the North Caucasus region. The purpose of the current study was to identify the Septoria leaf blotch resistance gene Stb2 in the collection material of winter durum wheat. The study was carried out in 2017–2018 in the laboratory of marker breeding of the FSBSI “ARC “Donskoy” in Zernograd. In the course of the study there have been applied such methods as the isolation of genomic DNA by the CTAB-method, PCR-analysis with the molecular SSR marker WMS533, electrophoresis on 2% agarose gel, analysis of electrophoresis gels in the Bio-Rad Image Lab 6.0 software, general data analysis by Microsoft Excel. The study has shown that 29 samples out of 185 analyzed ones had the Septoria leaf blotch resistance gene Stb2, they are the lines L3557 h42, L3182 h7, L3335 h29, L3336 h43, L3408 h218 (Russia, FSBSI “Research Center of Plants named after P. P. Lukyanenko”), K-61620 (Russia), K-54455, DF 917 (Romania), K-61924, K-59179, K-51863, K-61869 (Moldova), K-63871 (Turkey), Kharkovskaya 909 (Ukraine), MVTD-15-99 (Hungary), 2196-1/04, 922/93, 589/13, 655/13, 683/13, 145/14, 322/14, 484/14, Gordeiform 1424/82 (Russia, FSBSI “ARC “Donskoy”), Beliy Parus (Ukraine), Belgorodskaya Yantarnaya (LLC “Sativa” in Belgorod, Russia), IR named after V. Ya. Yuriev (Kharkov, Ukraine)), Zhivitsa (Russia, Prikumskaya OSS), Pandur (Romania) and Elidur (Romania). There have been recommended using the identified samples of winter durum wheat with the Septoria leaf blotch resistance gene Stb2 in breeding programs
Synthesis and biological activity of N-phosphonacetyl-L-aspartate’s structural analogs N-(α-dietoxyphosphorylcyclopropylcarbonyl)-amino acids
No full textObjectives. With the development and improvement of new delivery systems for substances of various natures, organophosphorus compounds with an antimetabolic mechanism of action have become relevant again. A few examples of them are organophosphorus analogs of carboxylic acids, such as N-phosphonacetyl-L-aspartate (PALA) and N-phosphonacetyl-L-isoasparagine, both of which are bio-rationally developed analogs of the transition state of carbamoylaspartate in the biosynthesis of pyrimidine bases, which is catalyzed by the enzyme aspartate transcarbamoylase (ATCase). Despite their high activity, these compounds have not found widespread use as anticancer agents due to a large number of side-effects and low bioavailability. Given the emerging opportunities for the delivery of phosphate and phosphonate derivatives into target cells, obtaining more effective analogs of PALA seems to be an interesting and promising research objective. The goal of the present study was thus to synthesize and study the biological activities of novel PALA analogs that are derivatives of phosphonacetic acid.Methods. For directed work within the framework of the study, we used the molecular docking method, which allowed us to simulate the binding of N-(α-diethoxyphosphorylcyclopropylcarbonyl)-substituted amino acids to ATCase. The target compounds were synthesized using classical methods of organic synthesis. The obtained compounds’ cytotoxicity was probed in relation to cell lines of human breast cancer (MDA-MB-231), skin cancer (A-375), and glioblastoma (U-87 MG).Results. The synthesis of eight novel N-(α-diethoxyphosphorylcyclopropylcarbonyl)-substituted amino acids was carried out. A few of the synthesized derivatives were tested for anticancer activity, but none displayed significant cytotoxicity.Conclusions. N-(α-diethoxyphosphorylcyclopropylcarbonyl)-substituted amino acids are synthetically available analogs of PALA, a compound capable of strong interaction with ATCase. However, the compounds synthesized in this work did not display any pronounced anticancer properties. One of the reasons for the observed low activity may be the presence of ether groups in the phosphonate building block
Содержание масла в семенах нута Национальной коллекции Украины
No full textChickpea (Cicer arietinum L.) is an important legume crop grown and consumed worldwide. Oil content in chickpea seeds ranges from 4 % to 7 % according to various data sources. Considering the interest of breeders in this issue, as well as for the purpose of inventory of the presented chickpea genetic material in the collection of the National Center for Plant Genetic Resources of Ukraine, these studies were carried out. Field experiments were carried out in 2016-2018 in the scientific crop rotation of the Plant Production Institute n.a. V. Ya. Yuriev of NAAS (Kharkiv, Ukraine). 43 samples (21 - kabuli type and 22 - desi type) of different ecological and geographical origin were studied. Oil content in chickpea seeds was determined using gravimetric method of S. V. Rushkovsky (Yermakov, 1987) in the laboratory for genetics, biotechnology and quality of the Plant Production Institute n.a. V. Ya. Yuriev of NAAS. On average, over the years of study, in the kabuli type accessions, the oil content level in the seeds made 7.08 %; for accessions - 6.05 %. The range of variability of this trait for the kabuli chickpea ranged from 5.22 % to 8.69 %, and for desi - from 4.40 % to 7.26 %. A low variability of the studied trait was noted for both the kabuli (V = 6.88-15.04 %) and for desi (V = 8.98-14.15%) chickpea cultivars. The advantage in terms of oil content in seeds, regardless of the growing conditions, was retained for the kabuli type. The accessions with the maximum level of the “oil content in seeds” trait manifestation were selected as “reference” for each type: for kabuli - variety Pamyat (Ukraine) - 7.95 %, for desi - Yarina (Ukraine) - 7.13 %. The best oil-bearing chickpea samples can be used in specialized programs to create new genotypes with a higher oil content in seeds.Нут (Cicer arietinum L.) является важной зернобобовой культурой, которую выращивают и потребляют во всем мире. Содержание масла в семенах нута по различным данным колеблется от 4 до 7 %. Учитывая интерес к данному вопросу со стороны селекционеров, а также с целью инвентаризации представленного генетического материала нута в коллекции Национального центра генетических ресурсов растений Украины были проведены настоящие исследования. Полевые опыты были проведены в 2016–2018 гг. в научном севообороте Института растениеводства им. В.Я. Юрьева (Харьков, Украина). Исследовали 43 образца (21 тип kabuli и 22 типа – desi) разного эколого-географического происхождения. Содержания масла в семенах нута определяли гравиметрическим методом С.В. Рушковского (Ермаков, 1987) в лаборатории генетики, биотехнологии и качества Институра растениеводства им. В.Я. Юрьева НААН. В среднем за годы изучения у образцов морфотипа kabuli содержание масла в семенах составило 7,08 %; у образцов типа desi – 6,05 %. Диапазон изменчивости данного признака для морфотипа kabuli колебался от 5,22 до 8,69 %, а у desi – от 4,40 до 7,26 %. Отмечена невысокая вариативность изучаемого признака как для морфотипа kabuli (V = 6,88–15,04 %), так и для desi (V = 8,98–14,15 %). Преимущество по содержанию масла в семенах, независимо от условий вегетации, сохранялось за морфотипом kabuli. В качестве «эталонных» для каждого из морфотипов выделены образцы с максимальным уровнем проявления признака «содержанием масла в семенах»: для морфотипа kabuli – сорт Память (Украина) – 7,95 %, для desi – сорт Ярина (Украина) – 7,13 %. Лучшие по масличности образцы нута могут быть использованы в специализированных программах для согдания новых генотипов с повышенным содержанием жира в семенах
Meglumine Sodium Succinate to Correct COVID-19-Associated Coagulopathy: the Feasibility Study
No full textAim of the study: to evaluate the effect of meglumine sodium succinate (MSS) on the efficacy of anticoagulant therapy in patients with severe COVID-19 infection complicated by bilateral community-acquired pneumonia.Materials and methods. Overall efficacy of treatment was analyzed in 12 patients hospitalized to ICU with the diagnosis of severe confirmed COVID-19 coronavirus infection (U07.1) complicated by bilateral multisegmental pneumonia. All patients received prophylactic anticoagulation with unfractionated heparin. The patients were divided into two groups: 7 of them received a multi-electrolyte solution containing MSS 5 ml/kg daily for the entire ICU stay (3-10 days) as a part of therapy; 5 patients received a similar volume of a conventional multi-electrolyte solution containing no metabolically active substrates and comprised a control group. Coagulation parameters were measured in arterial and venous blood of all patients at the following stages: 1) upon admission to the ICU; 2) 2-4 hours after the first dose of heparin; 3) 8-12 hours after the second dose of heparin; 4) 24 hours after the beginning of intensive therapy. On the 28th day of follow-up, mortality, duration of ICU stay, and incidence of thrombotic complications in the groups were evaluated. Nonparametric methods of statistical analysis were used to assess intragroup changes and intergroup differences.Results. The group of patients administered with MSS had significantly fewer thromboembolic events during 28 days of treatment and shorter ICU stay. These patients responded faster to anticoagulant therapy, which was suggested by more distinct changes in coagulation parameters, i.e. increased APTT, persisting viable thrombocyte population, reduced D-dimer and fibrinogen levels.Conclusion. The metabolic action of succinate possibly increases endothelial resistance to damaging factors and reduces its procoagulant activity. The hypothesis requires testing in a larger clinical study with a design including laboratory evaluation of the efficacy of varying doses of the studied drug as well as aiming at elucidation of the mechanisms of its effect on specific pro- and anticoagulation system components
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Stages and Fracture Mechanisms of Lamellar Pearlite of 100-m-Long Differentially Hardened Rails Under Long-Term Operation Conditions
No full textUsing the methods of transmission electron microscopy, the carbide phase evolution in surface layers of the differentially quenched rails is studied after the passed tonnage of 691.8 million tons at the depth up to 10 mm along the central axis and fillet of rail head. The action of two mutual supplement mechanisms of steel carbide phase transformation in surface layers at rail operation is established: (1) cutting mechanism of cementite particles with the following departure in the volume of ferrite grains or plates (in pearlite structure); (2) cutting mechanism and following dissolution of cementite particles, transfer of carbon atoms on dislocations (in Cottrell atmospheres and dislocation cores), transfer of carbon atoms by moving dislocations into ferrite grains volume (or plates) with the following repeated formation of nanosized cementite particles. The first mechanism is accompanied by the change in linear sizes and morphology of carbide particles. Cementite element composition change is not essential. Carbide structure change can take place during the second mechanism
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