134 research outputs found
Erratum: High-efficiency gene transfer into adult fish: A new tool to study fin regeneration (Genesis (January 2002) 32 (27-31))
No full textHigh-Efficiency Gene Transfer into Adult Fish : a New Tool to Study Fin Regeneration
No full textZebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to " knockdown " gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporation-based approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration
Comparison of sequentially measured Aloka echo-tracking one-point pulse wave velocity with SphygmoCor carotid-femoral pulse wave velocity.
No full textObjectives: Recently, echo-tracking-derived measures of arterial stiffness have been introduced in clinical practice for the
assessment of one-point pulse wave velocity. The purpose of this study was to find a relation between carotid–femoral pulse
wave velocity and one-point carotid pulse wave velocity, and to find a value of one-point carotid pulse wave velocity that
predicts carotid–femoral pulse wave velocity higher than 12 m/s.
Methods: A total of 160 consecutive subjects (112 male/48 female, mean age = 51.5 ± 14.1 years; 96 healthy, 44 hypertensives,
13 with aortic valve disease, and 7 with left ventricular dysfunction) were studied. Carotid–femoral pulse wave velocity was
measured with the SphygmoCor system and one-point carotid pulse wave velocity with high-definition echo-tracking system
(ProSound Alpha10; Aloka, Tokyo, Japan).
Results: Both carotid–femoral pulse wave velocity and one-point carotid pulse wave velocity correlated significantly with
each other (r = 0.539, p < 0.001) and with age (one-point carotid pulse wave velocity r = 0.618, carotid–femoral pulse wave
velocity r = 0.617, p < 0.0001 for both). Median value of carotid–femoral pulse wave velocity (7.2 m/s, 95% confidence interval
= 6.2–8.9) was systematically higher than that of one-point carotid pulse wave velocity (5.8 m/s, 95% confidence interval =
5–6.6). The area under the receiver operating characteristic curve was 0.85, identifying the cutoff for one-point pulse wave
velocity of 6.65 m/s as the best predictor of carotid–femoral pulse wave velocity more than 12 m/s (sensitivity = 0.818,
specificity = 0.819).
Conclusions: One-point carotid pulse wave velocity correlates with carotid–femoral pulse wave velocity, and the cutoff of
6.65 m/s was the best predictor of carotid–femoral pulse wave velocity over 12 m/s
AORTIC ROOT DIAMETERS AND AORTIC REGURGITATIONS IN HYPERTENSIVE PATIENTS COMPARE TO A CONTROL GROUP OF NORMAL SUBJECTS
No full textONE-POINT LOCAL ARTERIAL STIFFNESS IN SUBJECTS WITH CHRONIC SPINAL CORD INJURY AND NO OVERT CARDIOVASCULAR DISEASE
No full textNormal Values and Differences in Ascending Aortic Diameter in a Healthy Population of Adults as Measured by the Pediatric versus Adult American Society of Echocardiography Guidelines
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Right ventricular function in patients with systemic sclerosis without pulmonary arterial hypertension
No full textCarotid Artery Stiffness and Diastolic Function in Subjects without Known Cardiovascular Disease
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