1,721,365 research outputs found
<i>OSH2</i> deletion in Δ-s-tether cells does not impact growth.
No full textWT (SEY6210), osh4Δ Δ-s-tether (CBY5988) and osh2Δ Δ-s-tether (CBY6734) cells that contain SCS2 on a URA3-marked plasmid (pSCS2) were streaked onto solid growth medium. Cells were cultured for 4 days at 30°C on growth medium (containing 5’-FOA) to select against the SCS2-containing plasmid (-SCS2). As compared to growth on standard synthetic medium (+SCS2), osh4Δ Δ-s-tether cells do not growth in the absence of SCS2, whereas growth of osh2Δ Δ-s-tether cells is not dependent on SCS2. (TIF)</p
KEGG pathway and heatmap analysis of <i>osh4-1</i><sup>ts</sup> Δ-s-tether, Δ-s-tether, and <i>osh4-1</i><sup><i>ts</i></sup> <i>osh</i>Δ genomic expression.
No full text(A) Gene expression relative to WT (SEY6210) in osh4-1ts Δ-s-tether (CBY6031), Δ-s-tether (CBY5838), and osh4-1ts oshΔ (CBY926) cells listed by KEGG category. (B) Relative to WT, heatmap analyses of transcriptional responses in osh4-1ts Δ-s-tether, Δ-s-tether, and osh4-1ts oshΔ cells affecting inositol metabolism and regulation, lipid metabolism, and ERAD gene expression. Blue bars indicate transcriptional induction and red indicates repression. (TIFF)</p
Correlative analysis of <i>osh4-1</i><sup><i>ts</i></sup> Δ-s-tether, Δ-s-tether, and <i>osh4-1</i><sup><i>ts</i></sup> <i>osh</i>Δ transcriptomic profiles.
No full textCorrelation matrix of relative transcript abundance in osh4-1ts Δ-s-tether (CBY6031), Δ-s-tether (CBY5898), and osh4-1ts oshΔ (CBY926) cells relative to WT (SEY6210) grown in synthetic minimal medium at 30°C; osh4-1ts Δ-s-tether and osh4-1ts oshΔ cells were then incubated at 37°C for 1 h, as was their comparative WT control. Pearson correlations of corresponding genotypes as shown. (TIF)</p
Transcriptomic profiles of <i>osh4-1</i><sup><i>ts</i></sup> Δ-s-tether, Δ-s-tether and <i>osh4-1</i><sup><i>ts</i></sup> <i>osh</i>Δ cells.
No full textVolcano plots showing relative transcript abundance in (A) osh4-1ts Δ-s-tether (CBY6031), (B) Δ-s-tether (CBY5898) and (C) osh4-1ts oshΔ (CBY926) cells grown in synthetic minimal media at 37°C. Plots show log2-fold expression change relative to WT (SEY6210) versus the negative log10-P value (y-axis). Transcript changes log2 ≥ or ≤ 1 are shown in black whereas representative stress pathway genes are blue and red, corresponding to induction or repression, respectively. (D) Venn Diagram showing overlapping subsets of upregulated (blue) or downregulated genes (red) in osh4-1ts Δ-s-tether, Δ-s-tether and osh4-1ts oshΔ cells.</p
<i>DGK1</i> is a multicopy suppressor of <i>osh4</i> Δ-s-tether lethality that alleviates rESR gene repression.
No full text(A) Left: WT (SEY6210) and osh4Δ Δ-s-tether cells (CBY5988) that contain SCS2 on a URA3-marked plasmid (pSCS2), transformed with a high-copy 2μ plasmid expressing DGK1 (pCB1346) or a vector control (YEplac181), onto solid growth medium. Cells were cultured for 5 days at 30°C on growth medium (containing 5’-FOA) to select against the SCS2-containing plasmid (-SCS2). As compared to growth on standard synthetic medium (+SCS2), osh4Δ Δ-s-tether cells are only viable when DGK1 was present without SCS2. Right: Similar to above, tenfold serial dilutions of WT and osh4Δ Δ-s-tether cells containing both the SCS2 plasmid and high-copy DGK1, or the vector control, spotted on solid growth media with 5’-FOA (-SCS2) or without (+SCS2). High-copy DGK1 rescues the lethality of osh4Δ Δ-s-tether mutations. (B) Venn Diagram indicating numbers of upregulated (↑UP) or downregulated genes (DOWN↓) in osh4-1ts Δ-s-tether cells with (+ DGK1; purple) or without (- DGK1; light red) high-copy DGK1 plasmids at 37°C for 1 h, relative to WT. (C) Heatmap analyses of iESR and rESR transcriptional responses in osh4-1ts Δ-s-tether cells with or without high-copy DGK1 expression relative to WT, at 37°C for 1 h. Downregulated genes shown in red; upregulated genes shown in blue. (D) Graphical representation of the distribution of iESR and rESR gene responses in osh4-1ts Δ-s-tether, with and without high-copy DGK1 expression. (E) Tenfold serial dilutions of WT and Δ-s-tether (CBY5838) cells transformed either with vector or high-copy DGK1, and osh4Δ Δ-s-tether cells suppressed with high copy DGK1 (CBY6506). Cells were grown at 30°C on synthetic medium with or without 4 mM DTT for 4–5 days.</p
Expression of <i>OSH</i>, tether, and high-copy suppressors genes in Δ-s-tether, <i>osh4-1</i><sup>ts</sup> <i>oshΔ</i>, <i>and osh4-1</i><sup>ts</sup> Δ-s-tether cells.
No full text(A) Transcriptional expression of OSH1-OSH7 in Δ-s-tether (CBY5838) cells relative to WT (SEY6210) cells cultured with or without 75 μM inositol. (B) Transcriptional expression of genes encoding primary tether proteins in osh4-1ts oshΔ (CBY926) cells relative to WT cells cultured with or without inositol. (C) DGK1 expression in osh4-1ts oshΔ, Δ-s-tether, and osh4-1ts Δ-s-tether (CBY6031) cells relative to WT. (D) Transcriptional expression of ER-PM tether genes in osh4-1 (CBY7177) and osh4-1ts oshΔ cells relative to WT. (TIFF)</p
Multicopy <i>OSH</i> gene suppressors of <i>osh4-1</i><sup>ts</sup> Δ-s-tether.
No full textTenfold serial dilutions of WT (SEY6210), Δ-s-tether (CBY5898), and osh4-1ts Δ-s-tether (CBY6031) transformed with the 2μ plasmid control (YEplac195), OSH1 (pCB240), OSH2 (pCB239), OSH3 (pCB238), OSH4 (pCB241), OSH5 (pCB242), OSH6 (pCB237), or OSH7 (pCB236). Cells were grown on synthetic complete medium for 3–5 days at 23 or 37°C (TIFF)</p
iESR and rESR gene expression in Δ-s-tether, <i>osh4-1</i>, <i>osh4-1</i><sup>ts</sup> <i>oshΔ</i>, <i>and osh4-1</i><sup>ts</sup> Δ-s-tether cells.
No full textHeatmap analyses of transcriptional responses relative to WT (SEY6210) at 37°C for 1 h in osh4-1 (CBY7177), osh4-1ts Δ-s-tether (CBY6031), Δ-s-tether (CBY5898), and osh4-1ts oshΔ (CBY926) cells affecting (A) iESR and (B) rESR genes. Downregulated genes are shown in red, upregulated genes are shown in blue. iESR and rESR regulated genes were curated from previous reports [52,87]. (TIFF)</p
Inositol effects on Δ-s-tether and <i>OSH</i> mutant cells.
No full text(A) Scatter plot analysis of gene expression in Δ-s-tether (CBY5838) cells, relative to WT (SEY6210), when cultured in the absence versus presence of exogenously added inositol. (B) Scatter plot analysis of osh4-1ts oshΔ (CBY926) gene expression, relative to WT, when cultured in the absence versus presence of exogenously added inositol. (C) Ten-fold serial dilutions of WT, Δ-s-tether, osh4-1ts oshΔ, osh1Δ osh2Δ osh3Δ (JRY6253), and osh4Δ osh5Δ osh6Δ osh7Δ (JRY6272) cells grown at 30 or 37°C on solid synthetic minimal media containing 75 μM myo-inositol, 1 mM choline, or 75 μM myo-inositol with 1 mM choline. (TIFF)</p
Transcriptional anticorrelation of specific gene groups in Δ-s-tether versus <i>osh4-1</i><sup>ts</sup> <i>osh</i> cells.
No full textAnticorrelation of transcriptional responses in Δ-s-tether (CBY5898) versus osh4-1ts oshΔ (CBY926) cells. Reciprocal expression of specific GO-term gene groups are shown either when reduced in osh4-1ts oshΔ cells but increased in Δ-s-tether cells, or increased in osh4-1ts oshΔ cells and decreased in Δ-s-tether cells. (TIFF)</p
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