1,721,027 research outputs found
Aptamers for diagnostics, environmental and food quality applications
No full textsummary of application of aptamers based bioanalytical approache
Aptamer-Based Detection of Plasma Proteins by an Electrochemical Assay Coupled to Magnetic Beads
No full textThe DNA thrombin aptamer has been extensively investigated,
and the coupling of this aptamer to different
transduction principles has demonstrated the wide applicability
of aptamers as bioreceptors in bioanalytical
assays. The goal of this work was to design an aptamerbased
sandwich assay with electrochemical detection for
thrombin analysis in complex matrixes, using a simple
target capturing step by aptamer-functionalized magnetic
beads. The conditions for the aptamer immobilization and
for the protein binding have been first optimized by
surface plasmon resonance, and then transferred to the
electrochemical-based assay performed onto screenprinted
electrodes. The assay was then applied to the
analysis of thrombin in buffer, spiked serum, and plasma
and high sensitivity and specificity were found. Moreover,
thrombin was generated in situ in plasma by the conversion
of its precursor prothrombin, and the formation of
thrombin was followed at different times. The concentrations
detected by the electrochemical assay were in
agreement with a simulation software that mimics the
formation of thrombin over time (thrombogram). The
proposed work demonstrates that the high specificity of
aptamers together with the use of magnetic beads are the
key features for aptamer-based analysis in complex matrixes,
opening the possibility of a real application to
diagnostics or medical investigation
Transgenes monitoring in an industrial soybean processing chain by DNA-based conventional approaches and biosensors
No full text
Improved analytical performances of aptamer-based sensing for thrombin detection Analytical Chemistry
No full textAptamer-based assays represent a modern and attractive approach in bioanalytical chemistry. The DNA thrombin aptamer has been extensively investigated, and the coupling of this aptamer to different transduction principles has demonstrated the wide applicability of aptamers as bioreceptors in bioanalytical assays. The goal of this work was to critically evaluate all the parameters that can influence the sensor performances by using the thrombin
aptamer immobilized onto piezoelectric quartz crystals.
The optimization of the immobilization and the binding protocol was of paramount importance, and improvements in analytical performances could be obtained by
optimizing simple steps in immobilization and assay conditions. Moreover, the work demonstrated the possibility of using aptamer-based sensors in complex matrixes,
opening the possibility of a real application to diagnostics or medical investigation
BIOSENSORI PIEZOELETTRICI A BASE DI DNA PER L'ANALISI DI ORGANISMI GENETICAMENTE MODIFICATI(OGM)
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