425 research outputs found
Fig. 4 in On the configuration and occurrence of 2,6-cyclocuparan-3-ols: Resolving a lasting discrepancy
No full textFig. 4. Unequivocally established stereostructures of cyclocuparanols found in M. polymorpha in this work.Published as part of Zlatkovi, Dragan B., c, Đorđevic, Miljana R., Zlatkovi, c & Radulovic, Niko S., 2023, On the configuration and occurrence of 2,6-cyclocuparan-3-ols: Resolving a lasting discrepancy, pp. 1-10 in Phytochemistry (113566) 207 on page 5, DOI: 10.1016/j.phytochem.2022.113566, http://zenodo.org/record/829050
Fig. 6 in On the configuration and occurrence of 2,6-cyclocuparan-3-ols: Resolving a lasting discrepancy
No full textFig. 6. IR fingerprint regions of cyclopropane cuparenols 1–4, isolated in this work, and structurally related cis- and trans-sabinene hydrate and trans-sesquisabinene hydrate. *IR spectrum of 12 was digitized from Terhune et al. (1975). The X-axis is in micrometers (μm), and the Y-axis represents reflectance.Published as part of Zlatkovi, Dragan B., c, Đorđevic, Miljana R., Zlatkovi, c & Radulovic, Niko S., 2023, On the configuration and occurrence of 2,6-cyclocuparan-3-ols: Resolving a lasting discrepancy, pp. 1-10 in Phytochemistry (113566) 207 on page 6, DOI: 10.1016/j.phytochem.2022.113566, http://zenodo.org/record/829050
Mitogen-activated protein kinase signaling in the hippocampus and its modulation by corticotropin-releasing factor receptor 2 : a possible link between stress and fear memory
No full textA coordinated activation of multiple interlinked signaling pathways involving cAMP-dependent protein kinase (PKA) and mitogen-activated extracellular signal-regulated kinases (Mek-1/2) regulates gene expression and neuronal changes underlying memory consolidation. In the present study we investigated whether these molecular cascades might mediate the effects of stress on memory formation. We also investigated the role of hippocampal corticotropin-releasing factor receptor 2 (CRF2) in stress-enhanced learning and molecular signaling mediated by PKA, Mek-1/2, and their downstream targets extracellularly regulated kinases 1 and 2 (Erk-1/2) and p90-ribosomal-s-kinase-1 (p90Rsk-1).Acute 1 hr immobilization was used as a stressful stimulus, and one-trial context-dependent fear conditioning was used as a model for associative learning. Training of BALB/c mice 3 hr after the end of immobilization resulted in an enhancement of conditioned fear, as indicated by significantly increased freezing behavior of stressed when compared with nonstressed mice. Interestingly, Erk-1/2 phosphorylation after conditioning of nonstressed and stressed mice depended on PKA and Mek-1/2, respectively. Intrahippocampal injection of the selective Mek-1/2 inhibitor U0126 or CRF2antagonist antisauvagine-30 (aSvg-30) prevented stress-enhanced fear conditioning and Mek-1/2-dependent activation of Erk-1/2 and p90Rsk-1. aSvg-30 did not affect the phosphorylation of the PKA regulatory subunit II of stressed mice. The molecular and behavioral effects of CRF2coincided with stress-induced upregulation ofCRF2mRNA.These results suggest that modulation of Mek-1/2-dependent signaling by hippocampal CRF2can be selectively involved in the delayed effects of stress on memory consolidation.</jats:p
Preparation of a water activation experiment at JET to support ITER
No full textFast neutron induced water activation will result in significant additional nuclear heating loads to cryogenic components in the ITER fusion reactor during operation, which are considered as an important issue in the ITER design. A water activation experiment has been proposed at the Joint European Torus (JET) which would enable measurements of radiation dose rates and 16N concentrations in water, activated in the most representative fusion environment achievable, and extrapolability to ITER conditions. This paper presents a study aimed at establishing the experiment feasibility, in terms of the identification and selection of an experimental location at JET, and the definition of an experimental setup enabling accurate measurements of the quantities of interest. A suitable experimental location in JET was identified in the basement of the JET Torus Hall. The expected 16 O(n, p)16N activation rates in the cooling water in the JET tokamak were determined through Monte Carlo particle transport calculations and the induced 16N activity in the water was propagated to the experimental location. Subsequent calculations were performed to determine the expected detector response to 16N γ rays for three different JET pulse scenarios. Two scintillator γ detectors were calibrated with standard γ calibration sources and tested with a 244Cm/13 C neutron source, also emitting 6.13 MeV γ rays, corresponding to the 16N γ ray energy. The presented work demonstrates the feasibility of a water activation experiment at JET and provides valuable information for the experiment design
Systematic Nucleo-Cytoplasmic Trafficking of Proteins Following Exposure of MCF7 Breast Cancer Cells to Estradiol
No full textLongitudinal S. aureus colonization in the LEAP Study: Relationship with eczema severity, resolution, IgE production and food allergy
No full textReply
No full textrefers to: (1) Brough HA, Caubet JC, Mazon A, Haddad D, Bergmann MM, Wassenberg J, Panetta V, Gourgey R, Radulovic S, Nieto M, Santos AF, Nieto A, Lack G, Eigenmann PA. Defining challenge-proven coexistent nut and sesame seed allergy: A prospective multicenter European study. J Allergy Clin Immunol. 2020 Apr;145(4):1231-1239. doi: 10.1016/j.jaci.2019.09.036. Epub 2019 Dec 20. PMID: 31866098. And (2) Elizur A, Nachshon L, Levy MB, Epstein-Rigbi N, Goldberg MR; Nut CRACKER study group. Translating tree nut cross-reactivity into clinical practice. J Allergy Clin Immunol. 2020 May;145(5):1480-1481. doi: 10.1016/j.jaci.2020.02.003. Epub 2020 Mar 26. PMID: 32223980
Plasma coagulation in cardiac surgery
No full textBackground: Cardiac surgery using cardiopulmonary bypass is a complex procedure, sometimes accompanied with excessive bleeding. The nature and pathophysiology of this bleeding is multifactorial and not completely understood. Fibrinogen is a final step in the coagulation cascade and its substitution can alter postoperative hemorrhage.
Aims: To investigate different aspects of plasma coagulation in cardiac surgery. Firstly, to assess potential changes in coagulation factor levels during surgery and its relation to bleeding. Secondly, to study plasma’s potential to generate thrombin postoperatively. Thirdly, to compare two different regiments to dose anticoagulant heparin in regard to thrombin building capacity. Fourthly, to investigate effects of recombinant human fibrinogen ex vivo.
Material and methods: In study I, coagulation factor activities were measured before and after surgery in a cohort of 57 patients undergoing first time elective coronary artery bypass graft (CABG). Study II comprises the same cohort, now measuring thrombin generation potential using calibrated automated thrombography (CAT). Study III is a prospective trial, randomizing 60 elective CABG patients to either anticoagulation with weight-based heparin dosing or using heparin and protamine titration with a bedside device. In study IV, plasma of 10 cardiac surgery patients was spiked with various concentrations of human plasma derived fibrinogen or recombinant human fibrinogen. Ex vivo clot formation was assessed by rotational thromboelastometry.
Results: There is pronounced variation in level of individual coagulation factors after surgery. Concentration of fibrinogen and FXIII showed a weak correlation to bleeding volume 12 hours post surgery. Pronounced deterioration of thrombin generation capacity was found, possibly caused by persistent heparin effect and/or heparin rebound. Different heparin dosing regiments had no effect on per- and postoperative plasma’s thrombin generation capacity or on bleeding volume. Ex vivo, there is no difference between the new recombinant human fibrinogen concentrate and the one plasma derived regarding clot formation ability.
Conclusions: Postoperative decline (or rise) of individual coagulation factors does not seem to affect the postoperative bleeding volume, with reservation for fibrinogen and FXIII. Heparin effect is still present at 2 and 4 hours after surgery, affecting thrombin generation. More precise heparin and protamin dosing does not influence this phenomenon. Recently manufactured recombinant human fibrinogen concentrate is able to generate a clot of similar viscoelastic properties as the one plasma derived
Characterization of the underwater noise produced by recreational and small fishing boats (<14 m) in the shallow-water of the Cres-Lošinj Natura 2000 SCI
No full textRecreational boats are a dominant source of underwater noise in coastal areas, but reliable boat noise assessment is generally lacking. Here the Underwater Radiated Noise (URN) of seven recreational and small fishing boats moving at two different speeds was measured in the shallow waters of the Cres-Lošinj Natura 2000 SCI (Croatia). Measurements were undertaken considering the internationally recognized standards and published guidelines for shallow waters. URN was provided in 1/3 octave band spectra and in narrow band spectra, to highlight the tonal components. Engine power and type rather than the boat length and design result to be more predictive of URN. Highest speeds induce highest noise levels only in a very limited frequency range and different boats with similar speed but different engines show a shift in the spectra. Relevance of the achieved results for the tested area is further discussed
Folk horror as re-enchantment of a disenchanted world
No full textThe last few years have seen an increased interest in everything remotely connected to folk horror. Folk horror is a subgenre of horror dealing with metaphysical entities and occult dangers that are imagined as coming from a distant, pagan past. Often folk horror has a focus on agricultural customs and rituals. The paper will start with a discussion of folk horror and its characteristics, its use of folklore to (re-)create an enchanted world of strange traditions and rituals. Furthermore I propose a rereading of Phil Rickman’s Merrily Watkins Series about a female Anglican priest turned diocesan exorcist (14 tomes, since 1998) The focus will be on a) the respective use of folklore and folklore like stories (i.d. the folkloresque) that help to develop a sense of eerie danger and b) on the way these books are steeped in occult knowledge thus showing how this genre is part of occulture and contributes to a continued re-enchantment
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