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Applicazione della microscopia elettronica a scansione a pressione variabile im micologia: morfologia e dinamica di sviluppo delle strutture riproduttive nei Boleti
No full textLa microsopia elettronica a scansione a pressione variabile nello studio delle strutture riproduttive di Basidiomiceti.
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Variability on Catellatospora spp. strains isolated from plant materials
No full textNumerous strains of actinomycetes belonging to the "rare" genera were isolated from different plant materials as leaf litters and internal root cortical tissues; among them 53 were identified as members of the genus Catellatospora on the basis of the micromorphology of the reproductive structures. The variability characterizing these strains was studied, in comparison to 5 type strains of the genus, evaluating the cultural features on agar media and the ARDRA profiles obtained by three endonucleases Cfol, HaeIII and HpaII. The different profiles highlight the wide phenotypic and genotypic variability of these strains and the remarkable differences among most of them in comparison to the described ones. Only few strains showed characteristics similar to a type strain, in particular to Catellatospora citrea subsp. citrea, whereas several of them could belong to new taxa. The results achieved were also particularly remarkable under the ecological point of view considering the high number of studied wild isolates, in comparison to the knowledge on these genus based mostly on the descriptions of the few type strains. Furthermore the examined population was strictly connected with plant materials, in particular with leaf litter, instead the type strains were all isolated from woodland soil
Further studies on the etiological agents of carpinus betulus decline
No full textATP-binding cassette (ABC) transporters are a large superfamily of proteins responsible for the ATP-dependent translocation of a wide variety of different en-dogenous and exogenous toxicants across cellular membranes. We used a PCR-based approach to isolate from Botrytis cinerea a DNA fragment that proved be an ABC type transporter gene following sequencing and Southern blot analyses. This gene, named BcatrO, was characterized by RT-PCR expression and knock-out mutant phenotype analysis. Treatment of B. cinerea with H2O2 and methyl viologen enhanced transcription of BcatrO. An increase of the gene transcripts was also observed in the early stages of pathogenesis after inoculation of the fungus to bean leaves. BcatrO knock-out mutants of B. cinerea were more sensitive than the wild type both to H2O2 and fungicide fenpiclonil, but displayed the same virulence. The possible role of BcatrO in a mechanism of resistance of B. cinerea to reactive oxygen species (ROS) or their products and in pleiotropic drug resistance phenomena is discussed
Indagini istologiche ed istochimiche mediante tecniche di microscopia ottica ed elettronica per il riconoscimento e la caratterizzazione dei danni da ozono a livello ultrastrutturale
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