1,721,051 research outputs found
The energy dissipating systems of plant mitochondria are involved in plant defence against oxidative stress
No full textDifferent laser sensitivity of cytochrome c oxidase in isolated rat liver mitochondria irradiated either in the presence or the absence of respiratory chain inhibitors
No full textPlant mitochondrial Potassium Channel (PmitoKATP) and Uncoupling Protein (PUMP) in purified mitochondria are sensitive to reactive oxygen species
No full textLa citocromo ossidasi in mitocondri isolati è sensibile alla luce di un laser ad Elio Neon
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Specific helium-neon laser sensitivity of the purified cytochrome c oxidase
No full textPurpose: In order to gain some insight into the mechanism of interaction between Helium-Neon (He-Ne) laser light and mitochondrial cytochromes, the sensitivity of cytochrome electron transfer activity to He-Ne laser was tested. Materials and methods: Irradiation of solutions containing either purified cytochromes or dissolved rat liver mitochondria was carried out (wavelength 632.8 nm, fluence rate 10 mW cm-2, fluence 2 J cm-2); the irradiation conditions were the ones able to affect cytochrome c oxidase (COX) activity in mitochondria. Results: Cytochrome c oxidation catalysed by COX was affected by He-Ne laser irradiation of the purified enzyme. This result was obtained from measurements of the pseudo-first-order kinetic constant and from determinations of the turnover number of the enzyme, performed at different cytochrome c/COX ratios. Consistently, the kinetic parameters of COX changed. On the contrary, no alteration in the rate of electron transfer catalysed by either cytochrome c or bc1 complex was found. Conclusions: This study shows that purified COX is a specific target of He-Ne laser light; therefore, COX may be considered to be a mitochondrial photo-acceptor
The energy dose dependence of the activity of glutamate dehydrogenase irradiated with Helium Neon laser
No full textPurified glutamate dehydrogenase can interact with both coherent and non-coherent red light
No full textI mitocondri purificati da grano duro sono sensibili alla radiazione del laser ad Helium-Neon
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