3 research outputs found
INSILICO CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASES AND THEIR INTERACTION STUDY IN MULBERRY SILKWORM, BOMBYX MORI.
No full textThe Glutathione S-transferases are cytosolic enzymes that are found in both prokaryotes and eukaryotes. The GST multigene family are phase II detoxification enzymes that mainly helps to detoxify the large range of xenobiotic compounds in many organisms. These enzymes play a major role in detoxification pathway along with their related genes. We characterized the each GSTs class of silkworm such as Delta, Epsilon, Omega, Theta, Zeta and the phylogentic tree of GSTs were consructed. Further, we identified the conserved domains of GSTs such as thioredoxin_like and GST_C_family superfamily. Also, the G-site, H-site and dimer inferace of GSTs were identified. Around 10 conserved motifs were found by analyzing all GSTs of the silkworm. The silkworm genome sequences were mapped into the kegg pathway database and found the silkworm genes that are found in the xenobiotics metabolism pathway. Additionally, 128 protein-protein interactions of silkworm were found from the GSTs and their related genes of metabolic pathways. Further, functional enrichment analysis was shown. This study paves ways to understand the phase II detoxification enzymes, glutathione S-transferases and xenobiotics detoxification metabolic pathway of the mulberry silkworm.</p
Profiling of nutrients and bioactive compounds in the pupae of silkworm, Bombyx mori.
No full textThe mulberry silkworm, Bombyx mori pupae, is a major by-product of the silk reeling industry and has received
considerable attention in recent years due to the presence of various essential nutrients and bioactive compounds.
The crude protein content in dried pupae ranged from 51 to 55% and contains most of the essential
amino acids. The lipid content of dried pupae is 25 to 32%, and there are 11 kinds of fatty acids, among which
the content of α-linolenic acid is relatively high. Desirable levels of inorganic elements have detected, heavy
metals were in below the permissible range. Sugar analysis revealed the presence of 15 different sugar molecules,
of which sorbitol is the main component, and silkworm pupae also contains most of the water-soluble and fatsoluble
vitamins, including vitamins C, E, K, D and vitamin B12 forms. A total of 18 different compounds of
phenolic acids, mainly ferulic acid and cinnamic acid, and 15 different compounds of flavonoids, including
luteolin, quercetin, myricetin, catechin and epicatechin have detected in silkworm pupae. Comprehensive
analysis of nutritional and bioactive compounds in silkworm pupae revealed a wide variety of compounds with
potential to be explored in pharmaceuticals, nutraceuticals, functional foods, and feed additives
