1,721,057 research outputs found
Diarrea neonatale bovina : Escherichia coli e il fenomeno dell’antibiotico-resistenza
No full textEscherichia coli is a normal inhabitant of the gastrointestinal tract of humans and animals. Several highly adapted clones have acquired specific virulence characters and have become pathogenic. Pathotypes of E.coli represent the main cause of health problems and economical losses in calves. In the last years E. coli strains resistant to multiple drugs have been found, especially in calves affected by neonatal diarrhoea. Aim of the work was to evaluate the trend of antibiotic resistance of some E. coli strains isolated from calves. Results show a significant prevalence of multi antibiotic resistance in E. coli strains. Furthermore, the increasing level of resistance to antimicrobial agents important in treating human diseases, such as cephalospotins and fluoroquinolones, may represent a significant public health concern
Prevalence of feline bartonellosis and multilocus sequence typing of Bartonella henselae isolates in urban stray cats living in Milan, Italy
No full textCat scratch disease is a worldwide zoonosis caused predominatly by Bartonella henselae and in a lesser extent by B. clarridgeiae. Cats are the natural reservoir and vectors for B. henselae and B. clarridgeiae infections in humans. Genetic heterogeneity of B. henselae strains has been reported and multiple sequence types (STs) have been identified by the use of multilocus sequence typing (MLST). Particular sequence types have been more frequently associated with zoonosis than others. The aim of this study was to evaluate the prevalence of B. henselae and B. clarridgeiae infection in stray cats from Milan, Italy and to explore the genotypes of the B. henselae population for the evaluation of the potential risk of transmission to humans. Whole blood samples collected from 89 stray cats were cultured and analyzed by PCR. Sequence types of the feline B. henselae isolates were delineated using MLST. Bartonella henselae was detected in four (4.5%) cats and B. clarridgeiae was detected in one (1.1%) cat by PCR on blood samples. Coinfection by B. henselae type I and type II was identified in one cat. Four B. henselae isolates were cultured and were characterized as ST1 (2/4), ST5 (1/4) and ST8 (1/4), that are more commonly regarded as human associated or zoonosis associated STs. Typical feline associated B. henselae STs were not observed. Despite the low prevalence of B. henselae infection in stray cats from Milan, further investigation are needed to assess the risk for human health
Antibiotic resistance and patogenicity in Escherichia coli
No full textEscherichia Coli is a Gram negative bacterium, widely studied because it represents an integrating part of the human enteric flora, even if various strains are pathogen. Moreover such strains are zoonotic agents and they can be isolate also in ruminants in which cause diarrhoea and edema. Human infection occurs via fecal-oral pathway and animals are reservoirs for this human pathogen. Lesions are characterized by intimate bacterial attachment to the host cell membrane and the destruction of microvilli at the site of bacterial adherence, caused by the accumulation of signal proteins leading to the rearrangement of cytoskeletal proteins, in particular, filamentous actin, resulting in pedestal formation at the apical cell membrane.
In recent data, the evaluation of membrane proteins, phosphoproteome and the study of
oxidative stress, can contribute to understanding the phenomenon of antibiotic resistance to
molecular level and to define new strategies for the design of highly selective therapeutic
agents.
Evaluation of protein profiles respect to various mechanisms of stress, i.e. the resistance to
antibiotics or the modification related to the antibiotic resistance, represents a valid and
integrating approach for the study of new therapeutic strategies.
In the current study, comparative proteomics was applied to identify changes in proteins
responsible for antibiotic resistance in different in vivo isolates Escherichia coli. In particular
it has been studied strains with same virulence factors, but an antibiotic profile completely
different, isolates from different organs of the same animal
Proteomic analysis of multidrug resistance in Escherichia coli
No full textThe worldwide emergence of antimicrobial-resistant bacteria poses a serious threat to human health. The understanding of the mechanisms of antimicrobial resistance is extremely important for the control of these bacteria. Multidrug-resistant bacteria have frequently been reported, but information regarding proteome and its roles in the regulation of multidrug resistance is not yet available. In the current study, proteomic methodologies were used to characterize the proteome of multidrug-resistant Escherichia coli. Strains of Escherichia coli O157, O128, O111 and O26 isolated from buffalo feces and tested using antibiotic disk susceptibility methods were analyzed. Altered proteins of these E. coli strains were identified by 2-D gel based proteomic methodologies. The changes at the protein expression level detected by 2-D gel electrophoresis were validated by Mass Spectrometry. The information obtained from this study provides novel insights into mechanisms of antibiotic resistance
Serological testing to monitor immunity to canine vaccines: an useful tool in veterinary practice
No full textApplicazione della tecnologia DNA-microarray per la diagnosi e la caratterizzazione dei virus responsabili di neuropatologie negli equini
No full textMicroarray per l'identificazione simultanea di Equid herpesvirus, West Nile virus e Borna disease virus in equini
No full textPrevenzione delle EST (Encefalopatie spongiformi trasmissibili) con peptidi sintetici: effetti sulla progressione della patologia
No full textAntibody titers against panleukopenia, herpesvirus and calicivirus infections in stray cats of Milan
No full textA Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for feline coronavirus
No full textBACKGROUND
Loop-mediated isothermal amplification (LAMP) is a gene amplification technique that amplifies DNA in only one hour and under isothermal conditions, using a DNA polymerase with strand displacement activityand six primers targeting eight regions of the template sequence.
AIM OF THE WORK
The aim of this study was to develop a reverse transcription loop-mediated isothermal amplification assay for a rapid and inexpensive detection of feline coronavirus (FCoV).
METHODS
Six primers binding the 3' untranslated region (3'UTR) of the FCoV were designed. Thirty-two samples of RNA from cats (11 feces, 8 effusions, 9 blood samples and 4 tissues) were analyzed and a nested reverse transcription polymerase chain reaction (nRT-PCR) targeting the 3'UTR was also performed. The reaction mixture was incubated in a thermocycler at 63°C for 1 hour followed by 10 minutes at 80°C. LAMP results were evaluated both after electrophoresis migration on agarose gel stained with ethidium bromide and by naked eye after the addition of the hydroxynaphtol blue (HNB) dye. Results were compared with the nRTPCR, considered the gold standard.
RESULTS
The specificity was 100% on all specimens, while the sensitivity was 100% only on tissues. The overall sensitivity was 52.4% and 50% with gel electrophoresis and HNB, respectively. Discrepant results between the two visualization methods were recorded.
DISCUSSION/CONCLUSIONS
Except for tissues, the low sensitivity of the LAMP assay for FCoV limits a clinical application of this method. Additional experiments are needed in order to assess the analytical sensitivity and to develop a quantitative visualization technique
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