12,531 research outputs found

    Herb Wharton, Aboriginal stockman and author, Mount Isa, Queensland, 1994 /

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    Title devised by cataloguer from information provided by photographer.; Part of: Cattle Camp series of portraits of Aboriginal drovers, Mount Isa, Queensland, 1994.; Mode of access: Online

    A dual infection of infectious salmon anaemia (ISA) virus and a togavirus-like virus in ISA of Atlantic salmon Salmo salar in New Brunswick, Canada

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    Two viruses, infectious salmon anaemia (ISA) virus and a novel togavirus-like virus, were isolated from ISA disease outbreaks that were first reported as a new syndrome, haemorrhagic kidney syndrome (HKS) affecting farmed Atlantic salmon Salmo salar L. on the East coast of Canada. Laboratory confirmation of ISA diagnosis was initially complicated by isolation of only the togavirus-like agent using the CHSE-214 cell line. Here we demonstrate that a clinical sample from a disease outbreak of ISA contained a mixture of ISA virus and togavirus-like virus. Reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed the presence of both viruses during serial passage of cultures in SHK-1 and CHSE-214 cells. Virus harvested at passage level 3 in both cell lines caused high mortalities and severe gross pathology consistent with ISA virus infection in experimentally inoculated Atlantic salmon parr (approximately 35 g) in freshwater, beginning 12 d post inoculation. ISA virus was detected by virus isolation from kidney and liver tissues of all dead or moribund fish tested. A comparison of virus isolation, 1-step procedure RT-PCR and RNA dot-blot hybridization for detection of ISA virus (ISAV) in fish tissues showed virus isolation to have 100% sensitivity, followed by RT-PCR (66 and 28% sensitivity in kidney and liver, respectively), with RNA dot-blot hybridization as the least sensitive method (20 and 10% sensitivity in kidney and liver, respectively). No togavirus-like virus was detected in these samples by virus isolation. Moreover, another togavirus-like virus isolate grown in CHSE-214 cells in the absence of any other detectable pathogen was non-pathogenic in experimentally inoculated fish. This study confirms that the original ISA outbreaks in New Brunswick, Canada, were caused solely by ISAV

    Wirkungsbegriffe und Wirkungsverständnisse

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    Albus S, Greschke H, Klingler B, et al. Wirkungsbegriffe und Wirkungsverständnisse. In: ISA Planung und Entwicklung GmbH, ed. Praxishilfe zur wirkungsorientierten Qualifizierung der Hilfen zur Erziehung. Wirkungsorientierte Jugendhilfe. Vol 9. Münster: ISA; 2009: 20-21

    Wirkungsorientierung in wissenschaftlicher Perspektive

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    Albus S, Greschke H, Klingler B, et al. Wirkungsorientierung in wissenschaftlicher Perspektive. In: ISA Planung und Entwicklung GmbH, ed. Praxishilfe zur wirkungsorientierten Qualifizierung der Hilfen zur Erziehung. Wirkungsorientierte Jugendhilfe. Vol 9. Münster: ISA; 2009: 11

    Elemente Wirkungsorientierter Jugendhilfe und ihre Wirkungsweisen: Erkenntnisse der wissenschaftlichen Evaluation des Bundesmodellprogramms (Universität Bielefeld)

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    Albus S, Greschke H, Klingler B, et al. Elemente Wirkungsorientierter Jugendhilfe und ihre Wirkungsweisen: Erkenntnisse der wissenschaftlichen Evaluation des Bundesmodellprogramms (Universität Bielefeld). In: ISA Planung und Entwicklung GmbH, ed. Praxishilfe zur wirkungsorientierten Qualifizierung der Hilfen zur Erziehung. Wirkungsorientierte Jugendhilfe. Vol 9. Münster: ISA; 2009: 24-60

    Schlaglichter wirkungsorientierter Jugendhilfe. Fazit der Evaluation zum Bundesmodellprogramm „Wirkungsorientierter Jugendhilfe“

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    Albus S, Greschke H, Klingler B, et al. Schlaglichter wirkungsorientierter Jugendhilfe. Fazit der Evaluation zum Bundesmodellprogramm „Wirkungsorientierter Jugendhilfe“. In: ISA Planung und Entwicklung GmbH, ed. Praxishilfe zur wirkungsorientierten Qualifizierung der Hilfen zur Erziehung. Wirkungsorientierte Jugendhilfe. Vol 9. Münster: ISA; 2009: 6-7

    Car drivers’ preferences for ISA policy measures

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    Intelligent Speed Adaptation (ISA), an in-vehicle system that can either warn the driver or directly limit the vehicle’s speed when the speed limit is reached, is generally believed to have a large potential to increase road safety. However, policy makers hesitate to take policy measures that may increase ISA use. Public acceptance of ISA or policy makers’ perception of it is regarded to play an important role in this. This paper aims to increase this insight by reporting car drivers’ preferences for ISA policy measures based on stated choice experiment conducted in the Netherlands. Respondents made choices between various implementation strategies (mandatory ISA and voluntary ISA with financial incentives) given a chosen policy measure. The policy measure describes which drivers group (speed offenders, professional drivers or all drivers) is targeted and which ISA type (warning or limiting) is stimulated. The results point out that car drivers especially prefer that policy makers would impose ISA on speed offenders and to a lesser extent also on professional drivers, while they prefer a voluntary ISA for themselves. Use of voluntary ISA can be stimulated by offering financial incentives, of which purchase subsidy is preferred above annual tax cuts. Furthermore, car drivers prefer warning ISA for themselves and also for professional drivers, while they prefer limiting ISA for speed offenders. In addition, the results indicate that females and the older age group prefer ISA policy measures more than males and young drivers, but overall car drivers’ preferences seem to be rather homogeneous.Engineering, Systems and ServicesTechnology, Policy and Managemen

    Estimated genetic diversity between Atak-S and isa brown chickens with SSR markers

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    Ankara Tavukçuluk Araştırma Enstitüsü tarafından geliştirilen Atak-S yumurtacı hibriti yerli hibritler içinde yumurta verimi en yüksek olan hibrittir. Ancak bu hibritin yumurta verimi Isa Brown gibi yabancı kaynaklı hibritlere göre daha düşüktür. Atak-S hibritinin yabancı kaynaklı hibritlerle yarışabilmesi için verim ile ilgili moleküler ıslah çalışmaları gerekmektedir. Bu çalışmanın amacı Atak-S ve Isa Brown tavuk hibritlerindeki genetik çeşitliliği belirlemek ve bu hibritler arasındaki genetik çeşitliliği karşılaştırmaktır. Bu amaç için, iki alt popülasyondan 200 tavukta 6 mikrosatelit lokusu ayrı ayrı genotiplendirildi. Bu çalışmada kullanılan 6 farklı SSR markörü için toplam da 85 polimorfik fragment bulundu. Ayrıca He değeri 0.61±0.05 olarak bulundu. İki hibrit arasında He ise; Atak-S’de 0.35±0.76, Isa Brown’da 0.26±0.68 olarak bulundu. Bu sonuç, Atak-S hibritindeki genetik çeşitliliğin Isa Brown hibritine göre daha yüksek olduğunu ortaya koymuştur. Ek olarak iki hibrite ait genetik farklılıklar ve popülasyon yapısı dendogram analizi yapılarak ortaya konmuş ve farklıllıklar belirlenmiştir. Sonuç olarak Atak-S ve Isa Brown hibritleri içindeki ve arasında genetik çeşitliliklerin iyi korunmuş olduğu ileri sürülebilir. Mikrosatellit analizi ile tahmin edilen Atak-S ve Isa Brown hibrtileri hakkındaki bilgiler, gelecekteki genetik varyasyon araştırmalarını tasarlamak ve koruma stratejileri geliştirmek için hedeflerin belirlenmesinde rehber olarak yararlı olabilir.Atak-S layered hybrid, developed by the "Ankara Poultry Research Institute", is the hybrid with the highest egg productivity among local hybrids. However, egg yield are lower compared to foreign-origin hybrids such as Isa brown. Molecular development studies as to productivity are required for Atak-S hybrid to be able to compete with foreign-origin hybrids. The objective of this study was to detect genetic diversity within the breeds and to compare the Atak-S and Isa Brown breeds. To achieve this goal, we individually genotyped 6 SSR loci in 200 chickens from two populations. 85 polymorphic fragments were found in total for 6 different SSR markers employed in this study. For all loci, high He was observed, and means He was 0.61±0.05 among loci. Among breeds, the mean He was 0.35±0.76 in Atak-S and 0.26±0.68 in Isa Brown. This result indicated that genetic diversity in the Atak-S breed is higher than in the Isa Brown breed. Furthermore, genetic differences and population structure of two hybrids were shown by virtue of dendogram analysis and differences were detected. As a result, we could be suggested that genetic diversity is well preserved within and between Atak-S and Isa Brown hybrids. Based on the Atak-S and Isa Brown breeds estimated by microsatellite analysis may be useful as a guide in setting goals for designing future genetic variation studies and for improving conservation strategies

    What drives the Acceptability of Intelligent Speed Assistance (ISA)?

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    To have knowledge about the acceptability of Intelligent Transport systems (ITS) is most beneficial for the development of supported implementation strategies. So far, different theories and methods, also stemming from other domains, have been used to define and conceptualize the notion of acceptability. In a previous paper, we developed a theoretical concept to define acceptability of ISA based on different theories and methods used in ITS & ISA research. In the current paper we aim to find out which predefined indicators are relevant to define the acceptability of ISA. Background factors, contextual issues and ISA-device related factors are used as indicators to predict the level of acceptability. Structural Equation Modelling (SEM) is used to define the direct and indirect effects.Infrastructures, Systems and ServicesTechnology, Policy and Managemen

    Isa Lake on the Continental Divide, Yellowstone National Park.

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    Isa Lake on the Continental Divide, Yellowstone National Park
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