29 research outputs found
Flexible cystourethroscopy is the best way for monitoring patients with urethral stricture disease
No full text
Synthesis of High-Molecular-Weight Poly(ether-<i>alt</i>-ester) by Selective Double Ring-Opening Polymerization of Spiroorthoesters
No full textWe report the selective double ring-opening
polymerization of presequenced
spiroorthoester monomers to form high-molecular-weight (≈90
kDa) poly(ether-alt-ester)s with a simple cationic
alkyl gallium catalyst. The selective formation of double ring-opened
polymer units was confirmed by NMR and IR spectroscopies. Thermal
and rheological properties of homo- and copolymers were further characterized
by differential scanning calorimetry, thermogravimetric analysis,
and stress-controlled rotational rheometry. Linear viscoelastic moduli
show that these systems are well entangled (plateau modulus), thereby
possessing nearly terminal relaxation at long time scales (low frequencies)
and Rouse segmental dynamics at short time scales (high frequencies)
with characteristic slopes. These are the highest-molecular-weight
poly(ether-alt-ester)s reported to date
Synthesis of High-Molecular-Weight Poly(ether-<i>alt</i>-ester) by Selective Double Ring-Opening Polymerization of Spiroorthoesters
No full textWe report the selective double ring-opening
polymerization of presequenced
spiroorthoester monomers to form high-molecular-weight (≈90
kDa) poly(ether-alt-ester)s with a simple cationic
alkyl gallium catalyst. The selective formation of double ring-opened
polymer units was confirmed by NMR and IR spectroscopies. Thermal
and rheological properties of homo- and copolymers were further characterized
by differential scanning calorimetry, thermogravimetric analysis,
and stress-controlled rotational rheometry. Linear viscoelastic moduli
show that these systems are well entangled (plateau modulus), thereby
possessing nearly terminal relaxation at long time scales (low frequencies)
and Rouse segmental dynamics at short time scales (high frequencies)
with characteristic slopes. These are the highest-molecular-weight
poly(ether-alt-ester)s reported to date
Pro-apoptotic Bid induces membrane perturbation by inserting selected lysolipids into the bilayer
No full textBid is a BH3-only member of the Bcl-2 family that regulates cell death at the level of mitochondrial membranes. Bid appears to link the mitochondrial pathway with the death receptor-mediated pathway of cell death. It is generally assumed that the f.l. (full-length) protein becomes activated after proteolytic cleavage, especially by apical caspases like caspase 8. The cleaved protein then relocates to mitochondria and promotes membrane permeabilization, presumably by interaction with mitochondrial lipids and other Bcl-2 proteins that facilitate the release of apoptogenic proteins like cytochrome c. Although the major action may reside in the C-terminus part, tBid (cleaved Bid), uncleaved Bid also has pro-apoptotic potential when ectopically expressed in cells or in vitro. This pro-apoptotic action of f.l. Bid has remained unexplained, especially at the biochemical level. In the present study, we show that f.l. (full-length) Bid can insert specific lysolipids into the membrane surface, thereby priming mitochondria for the release of apoptogenic factors. This is most effective for lysophosphatidylcholine species that we report to accumulate in mitochondria during apoptosis induction. A Bid mutant that is not pro-apoptotic in vivo is defective in lysophosphatidylcholine-mediated membrane perturbation in vitro. Our results thus provide a biochemical explanation for the pro-apoptotic action of f.l. Bid. © 2005 Biochemical Society
Incidence and factors associated with anemia among the geriatric population at a tertiary care hospital in southern India
No full textINTRODUCTION: Anaemia in geriatric populations poses challenges to healthcare systems
worldwide. Hence, this study was done to investigate the incidence of anemia among the geriatric
population of South India.
METHODS: Retrospective, cross-sectional study with 144 patients aged 65 years and above.
Socio-demographic data and laboratory findings were recorded in a validated proforma format.
Data were analyzed using SPSS.
RESULTS: 36.1% of the study population was anemic, and 63.9% were non-anemic. The prevalence
of anemia was higher among females (36.36%) than males (35.82%). The prevalence of anemia
was significantly correlated with advanced age (r=0.21; p < 0.05) and female sex (r=0.25; p <
0.05). Thirty-six patients were mild anemic (69%), 13 had moderate (25%), and 3 (6%) had severe
anemia. Nutritional anemia was most common (80%), followed by hemorrhagic anemia (18%) and
hemolytic anemia (2%). Among the 52 patients who were anemic, the most common comorbidities
associated were Type 2 diabetes mellitus (69.2%) and hypertension (53.8%). Liver diseases were
present in 5 cases (9.62%), renal insufficiency in 14 cases (26.9%), hypercholesterolemia in 5 cases
(9.62%), and 6 cases (11.5%) were associated with other diseases. All the patients were managed
conservatively with treatment, and anemia improved in 36.5% of cases.
CONCLUSION: Anaemia was frequently diagnosed in the studied geriatric population. Nutritional
deficiencies were the most common cause, followed by hemorrhagic and hemolytic anemia. The
impact of anemia on quality of life, recovery from illness, and functional abilities must be further
investigated in future geriatric studies
Analysis of MD simulation trajectories of 20 ns time scale.
No full text(A) RMSD plot displaying the molecular vibration of Cα backbone of wild type (red), and G78S mutant (black). (B) RMSD plot displaying the molecular vibration of Cα backbone of wild type mitochondrial ATPase (black), and T146A mutant (red). (C) RMSF plots show the fluctuations of respective amino acids throughout the simulation time 20 ns wild type (red), and G78S mutant (black). (D) RMSF plots show the fluctuations of respective amino acids throughout the simulation time 20 ns wild type mitochondrial ATPase (black), and T146A mutant (red). (E) The radius of gyration plots for the deduction of compactness of protein wild type (red), and G78S mutant (black). (F) Radius of gyration plots for wild type mitochondrial ATPase (black), and T146A mutant (red). (G) Number of hydrogen bonds formed between G78 and chain B (red) and S78 and chain B (black) during 20 ns simulation time scale. Principal Component Analysis (PCA) of (H) wild type chain A domain and (I) G78S mutant, (J) wild type ATPase and (K) mutant T146A, into unordered (yellow) to ordered (black) clusters, while comparing PC1 and PC2 for 20 ns simulation trajectories.</p
Unique combinations of co-existing missense variants and pathogenicity prediction by SIFT and Polyphen2 and reported in CADD and APOGEE.
No full textVariants for which SNAP 2 score was >50 or con-surf value was 8 or more are shown in bold.</p
Distribution of macro-haplogroups and haplogroups among the 30 matched pairs subjected to NGS analysis.
No full textDistribution of macro-haplogroups and haplogroups among the 30 matched pairs subjected to NGS analysis.</p
Variants identified at >5% within either population (patients: N = 30; controls: N = 30) in the coding region of the mt genome by next generation sequencing excluding genomic regions <i>MT-ND3</i>, <i>MT-ND6</i>, <i>MT-ND4L</i>, <i>MT-CYB</i>, <i>MT-TT</i>.
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