1,720,983 research outputs found
Morphological Variation Analysis of the repeatability of soccer offensive schemes
No full textA landmark-based statistical method, morphological variation analysis, for the quantification of the repeatability in the arrangement of body segments during the execution of sport actions has recently been developed. A two-dimensional shape is produced and its morphology is studied. The method was used to measure the within-team variability of the relative positions of players during the execution of offensive schemes in soccer. Two junior soccer teams of different technical abilities (semi-professional vs. amateur), each playing two standardized offensive schemes of different difficulty (easier: throw-in; more difficult: wing attack) were filmed. Each scheme was repeated 25 (semi-professionals) or 10 (amateurs) times. For each repetition, the position of the players in a single significant frame was analysed using morphological variation analysis. The reproducibility of both schemes was higher among the semi-professionals than among the amateurs (two-way analysis of variance, P<0.005). The repeatability of the players' relative positions was related to the difficulty of the scheme and the technical level of the team. Among the amateurs, the throw-in was more reproducible than the wing attack (Student's t-test, P<0.005). The method not only allows the quantification of collective (team) coordination, but also the separation of the influence of individual player
Compartimenti intracellulari batterici e distribuzione intra-cellulare dei chemioantibiotici
No full text
A new model examining intracellular and extracellular activity of amoxicillin, azithromycin, and clarithromycin in infected cells
No full textAn in vitro infection model was created using a suspension of macrophages, polymorphonuclear leukocytes, lymphocytes, fibroblasts, and human serum to which pathogen and antibiotic were added. Separate intracellular and extracellular antibiotic concentrations and activity against Staphylococcus aureus and Legionella pneumophila were assessed for three antimicrobial agents: amoxicillin, azithromycin and clarithromycin. Amoxicillin was found almost exclusively in extracellular fluid, where it was active; intracellularly, it was ineffective. Azithromycin, in contrast, was primarily concentrated and active intracellularly, with little activity in extracellular fluid. Clarithromycin was present in both compartments and possessed significant activity both intracellularly and extracellularly
IN VITRO COMPARATIVE DYNAMICS OF MODIFIED RELEASE CLARITHROMYCIN AND OF AZITHROMYCIN
No full textAntibacterial kinetics of modified-release clarithromycin (CLA) and azithromycin (AZI) against respiratory tract pathogens were compared in relation to their pharmacokinetic profile. The study was carried out in three strains of Streptococcus pneumoniae, group A β-hemolytic Streptococcus pyogenes, Moraxella catarrhalis and Haemophilus influenzae, respectively, exposed to concentration gradients of CLA and AZI simulating human serum pharmacokinetics after administration of 500 mg p.o. in a single dose. Bactericidal kinetics were assessed by counting the number of survivors before each change in concentration over a period of 36 h. The minimal inhibitory concentrations (MICs) of CLA and AZI were evaluated at time 0 and after 36 h of exposure to antibiotics in the surviving organisms. The results showed that CLA and AZI, in the experimental conditions adopted, had different antibacterial kinetics. Moreover, the addition of the 14-OH metabolite of CLA at the same concentrations reached in human serum exerted a bactericidal effect against two strains of H. influenzae resistant to CLA and AZI. An increase in MICs was observed against S. pyogenes and H. influenzae, with higher values for AZI. Copyright (C) 2000 S. Karger AG, Basel
Ceftibuten concentrations in human tonsillar tissue
No full textIn a study designed to determine ceftibuten concentrations in tonsillar tissue, subjects scheduled to undergo tonsillectomy were administered 400 mg of ceftibuten in a single oral dose. Between 2 and 24 h after the dose was given, tonsillar tissue samples were taken during surgery and assayed for ceftibuten. Mean concentrations in tonsillar tissue 4.4 h and 24.6 h after the 400 mg dose were 5.3 ± 2.7 and 0.3 ± 0.3 mg/g, respectively. Concurrent mean serum concentrations were 7.42 ± 1.66 and 0.15 ± 0.13 mg/ml, respectively. The apparent half-life of drug in the tissue was 5.3 h. The presence of high ceftibuten concentrations in tonsillar tissue suggests that a once-daily regimen may be effective in treating tonsillitis and pharyngitis
Comparison of the potency of 10 different brands of Serenoa repens extracts
No full textBACKGROUND:
The extract of Serenoa repens is the phytopharmaceutical product most often used for the treatment of urological symptoms associated with benign prostatic hyperplasia (BPH). Several extracts are commercially available but extraction processes vary between manufacturers and thus not all these products are equivalent in terms of active ingredient content and composition of preparations.
AIM:
As there is a paucity of comparative studies, we compared the activity of different extracts of Serenoa repens widely available on the world market.
MATERIALS AND METHODS:
Beltrax Uno, Permicaps, Permixon, Prostadyn, Prostagutt, Prostamen, Prostamol Uno, ProstaX, Urocaps and Urogutt were assayed for 5-alpha-reductase activity on 10 day fibroblasts and epithelial cells cocultures. Human fibroblast growth factor (hFGF)-induced-proliferation inhibition was also assayed.
RESULTS:
As to extract activity, differences were observed between the tested extracts, but all were able to inhibit 5-a-reductase types I and II isoenzymes (5alphaR-I and 5alphaR-II) as well as fibroblast proliferation.
CONCLUSIONS:
Extract potency differs between products and so does proliferation inhibition potency. Quantitative and qualitative variations in the active ingredient are likely to account for these differences
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