142 research outputs found
Nurse Autonomy Pain Control and Discharge from Recovery
No full text Beverley Colwill, the author of this article, was faced with the question, ‘Is it always necessary for patients to remain in the recovery room for 30 minutes following their last intravenous bolus dose of morphine?’ To try to find an answer, she carried out the literature search which is reported here. </jats:p
Inheritance of evolved glyphosate resistance in Lolium rigidum (Gaud.)
No full textThe original publication can be found at www.springerlink.comResistance to the non-selective herbicide, glyphosate, has evolved recently in several populations of Lolium rigidum (Gaud.). Based upon the observed pattern of inheritance, glyphosate resistant and susceptible populations are most probably homozygous for glyphosate resistance and susceptibility, respectively. When these populations were crossed and the F1 progeny treated with glyphosate, the dose response behavior was intermediate to that of the parental populations. This observation, coupled with an absence of a difference between reciprocal F1 populations, suggests that glyphosate resistance is inherited as an incompletely dominant nuclear-encoded trait. The segregation of resistance in F12S backcrosses suggests that the major part of the observed resistance is conferred by a single gene, although at low glyphosate treatments other genes may also contribute to plant survival. It appears from this study that a single nuclear gene confers resistance to glyphosate in one population of L. rigidum.D. F. Lorraine-Colwill, S. B. Powles, T. R. Hawkes and C. Presto
Investigations into the mechanism of glyphosate resistance in Lolium rigidum
No full textGlyphosate is a widely used non-selective herbicide to which so far only three weed species have evolved resistance. Here, we report on the mechanism of glyphosate resistance in one resistant population of Lolium rigidum (Gaud.). Experiments demonstrate that glyphosate resistance in this population is directly correlated with increased transport of the herbicide to leaf tips. No significant differences in the level of expression of the herbicide target site, EPSP synthase, between resistant and susceptible plants were found and the enzyme is equally sensitive to inhibition by glyphosate in both populations. Similarly, plant metabolism of glyphosate does not contribute to resistance. Resistant and susceptible plants are equally capable of absorbing the applied herbicide. The most notable difference between resistant and susceptible populations is found in the translocation of glyphosate. Following treatment, an accumulation of glyphosate in the roots of susceptible plants is observed, whereas glyphosate accumulates in the leaf tips of resistant plants. Taken together, it seems likely that an alteration to the cellular transport of glyphosate confers resistance.D. F. Lorraine-Colwill, S. B. Powles, T. R. Hawkes, P. H. Hollinshead, S. A. J. Warner and C. Presto
Tolerance, intolerance, and fanaticism, W.D. Valgardson's reaction to the religious debate in New Iceland
No full textIn the 1870s, religious controversy and its resulting dissent created a division in the immigrant community of New Iceland. The heart of the religious debate centred on Pall porlaksson's orthodox teachings of the Norwegian Lutheran Synod and Jon Bjarnason's liberal views espoused by the Lutheran State Church of Iceland. The debate resulted in animosity between community members to the point that the community split. Although poor living conditions played some role, rancour over religious dissent was the primary dividing force, leading, in 1879, to a migration to North Dakota in the footsteps of porlaksson. This thesis will focus on the writings of Icelandic-Canadian author W. D. (William Dempsey) Valgardson and the religious events that took place in New Iceland long before his birth. It will show how Valgardson, drawing from this religious heritage, creates themes of tolerance, intolerance and fanaticism. Valgardson claims that a moral quality exists in his writing, a concern with Christian and non-Christian behaviour, and the use and abuse of religious power. This thesis will examine these themes in his novel, short stories, and poetry, in light of the historic events of religious strife and bonding in New Iceland
A roadmap to generate renewable protein binders to the human proteome
No full textDespite the wealth of commercially available antibodies to human proteins, research is often hindered by their inconsistent validation, their poor performance and the inadequate coverage of the proteome. These issues could be addressed by systematic, genome-wide efforts to generate and validate renewable protein binders. We report a multicenter study to assess the potential of hybridoma and phage-display technologies in a coordinated large-scale antibody generation and validation effort. We produced over 1,000 antibodies targeting 20 SH2 domain proteins and evaluated them for potency and specificity by enzyme-linked immunosorbent assay (ELISA), protein microarray and surface plasmon resonance (SPR). We also tested selected antibodies in immunoprecipitation, immunoblotting and immunofluorescence assays. Our results show that high-affinity, high-specificity renewable antibodies generated by different technologies can be produced quickly and efficiently. We believe that this work serves as a foundation and template for future larger-scale studies to create renewable protein binders
Extensive training, partial reinforcement, and temporal gaps do not affect S-S learning in second-order autoshaping
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Polyoma and Cell Chromatin Replication Studied in Mouse Cells which Exhibit Temperature-Sensitive DNA Synthesis because they are S ts or G1 ts
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