11,397 research outputs found

    Cha guan. [3 & 4]

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    1. 茶館 : side 3 -- 2. 茶館 : side 4.Section number supplied by cataloguer.Possibly reproduced from other commercial recording or radio broadcast (Pending for review)Electronic reproduction from Rulan Chao Pian Audio Cassette Collection.Spoken in Chinese.1. Cha guan : side 3 -- 2. Cha guan : side 4

    Cha guan. [1 & 2]

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    1. 茶館 : side 1 -- 2. 茶館 : side 2.Section number supplied by cataloguer.Possibly reproduced from other commercial recording or radio broadcast (Pending for review)Electronic reproduction from Rulan Chao Pian Audio Cassette Collection.Spoken in Chinese.1. Cha guan : side 1 -- 2. Cha guan : side 2

    Cha Cah Tana

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    Cha Cah Tana, head-and-shoulders portrait, facing front.Curtis no. 112.LC no. 26.Forms part of: Edward S. Curtis Collection (Library of Congress)

    CHA CHA A GOGO POUR LES FIDELES DE CASTRO / l'Orquesta Hermanos CASTRO

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    Titre uniforme : [Come prima]Titre uniforme : [Come prima]Titre uniforme : [Come prima]Comprend : TEMERIDAD : cha cha cha / M. JIMENEZ - SENORITA LUNA : cha cha cha / E. CASAS - PAYCA : guaracha / P. JAIMES - EL CHIPI CHIPI : mambo / R. d'ESCOT - COME PRIMA : cha cha / PANZERI - TACCANI - di PAOLA - ME VUELVO UN DENGUE : cha cha oriental / G. RAMIREZ - NENA : cha cha cha / N. JIMENEZ - MI GATICO VINAGRITO : cha cha cha / T. FERNANDEZ - LA ULTIMA NOCHE : mambo / B. COLLAZO - MAMBO CON CHA CHA CHA : mambo cha cha / S. RAMOS - OLVIDO : tango guapacha / L. RUBISTEINBnF-Partenariats, Collection sonore - BelieveContient une table des matière

    Cha-Cha-Cha: Variable Adhesive Activity of the <italic>Haemophilus</Italic> Cryptic Genospecies Trimeric Autotransporter Cha

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    Disease caused by the Gram-negative Haemophilus cryptic genospecies begins with colonization of the maternal genital or neonatal respiratory tract. The primary goal of this work was to identify and characterize the molecular determinant(s) of Haemophilus cryptic genospecies adherence as a means to better understand the specific adaptation of this species to the urogenital tract and neonatal respiratory tract. Using transposon mutagenesis of prototype strain 1595, we identified a locus that is essential for Haemophilus cryptic genospecies adherence to a variety of epithelial cell lines of both genital and respiratory origin. This locus encodes a protein called Cha that shares homology with trimeric autotransporters. Trimeric autotransporters are composed of an N-terminal signal peptide, an internal passenger domain that harbors adhesive activity, and a short C-terminal membrane anchor domain and are classically characterized by head-stalk-anchor domain architecture. By generating chimeric proteins, we demonstrated that the C-terminus of Cha trimerizes in the bacterial outer membrane and is capable presenting a heterologous passenger domain (Hia) in a functional form, thus confirming that Cha is a trimeric autotransporter. Southern analysis revealed that cha is unique to the Haemophilus cryptic genospecies and is ubiquitous among these strains. Similar to a number of trimeric autotransporters, the passenger domain of Cha contains scattered clusters of YadA-like head domains associated with head-to-stalk neck adaptor motifs, predicted coiled-coil stalks and a series of identical tandem coding repeats which are not required for adherence. By evaluating the adherence capacity of H. influenzae expressing Cha deletion derivatives, we established that the N-terminal 473 residues of Cha harbor the binding domains responsible for Cha-mediated adherence to epithelial cells. In additional studies, we demonstrated that this same N-terminal region mediates bacterial aggregation through inter-bacterial Cha-Cha binding. Further analysis revealed that variable Cha-mediated adherence is linked to spontaneous changes in the number of identical tandem repeats predicted to comprise a coiled-coil stalk domain. Variation in repeat copy number has a direct effect on Cha adhesive and aggregative activity, independent of an impact on transcription of the cha locus or surface localization of Cha protein. Moreover, length of Cha surface fibers correlates with repeat copy number expansion. We propose two hypotheses to explain how repeat expansion inhibits bacterial aggregation and host cell binding: 1) Variation in the number of 28-amino acid repeats may influence the conformation of Cha, thus changing the surface accessibility of the Cha binding pocket. 2) Repeat expansion results in the formation of long, flexible Cha fibers on the bacterial cell surface that may have a greater propensity to interact with neighboring Cha trimers at the N-terminus, thereby precluding adherence to other bacteria or host epithelial cells. In additional studies screening adherent cryptic genospecies isolates for expression of Cha protein, we identified an additional, antigenically-divergent Cha variant that we refer to as Cha2. Amino acid sequence and domain comparison of Cha2 with Cha (now Cha1) revealed that the structurally undefined N-terminal sequences (encompassing the Cha1 adhesive and aggregative domain) are strikingly divergent. Inspite of this, Cha2 mediates efficient adherence to human epithelial cells, similar to Cha1.Identification of Cha offers insight into the apparent tissue tropism associated with the Haemophilus cryptic genospecies. We speculate that the unique regulation of Cha adhesive activity enhances the adaptive capability of this pathogenic organism in the human host.</p

    TWIST, SLOW ET CHA-CHA-CHA

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    Titre uniforme : [Amor, amor]Titre uniforme : [Perfidia]Titre uniforme : [Perfidia]Titre uniforme : [Perfidia]Comprend : TWISTIN' U.S.A. / K. MANN - G. BERTRET et R. DESBOIS - DANSE LE TWIST AVEC MOI ("Dance the mess around" twist) / K. MANN - D. APPELL et J. DAMBROIS ; LES GUITARES DU DIABLE - WAP-DOU-WAP ("Tu aimes le twist") / JIL et JAN et Johnny HALLYDAY - SUNSET : slow-rock / WOODMAN ; Billy NASH ROCK BAND - DANIELA : slow-rock / G. GARVARENTZ et A. PASCAL ; TRUMPET BOY et sa trompette-succès - RIO BAMBA ("Cuba cha cha") / L. GONZALES et A. SALVET - BAILALO CHIQUITA : cha-cha / L. RECO - E. KERMONT et D. GALLEGO ; LOS MERECUMBES - REVE, MON REVE : boléro / Alec SINIAVINE et Pierre COUR ; Jean-Michel DEFAYE et son orchestre - PERFIDIA, boléro / DOMINGUEZ - CUGAT et HEAGNEY - AMOR AMOR : rock-béguine / G. RUIZ - S. SYKAR et R. LOPEZ MENDEZ ; les ALCARSON - VIENS DANSER LE TWIST ("Let's twist again") / K. MANN - D. APPELL et G. GOSSET ; TRUMPET BOY et sa trompette-succès - FICH' LE CAMP JACK ("Hit the road Jack") twist / P. MAYFIELD et G. ABER - THE FLY : twist / J. MADARA et D. WHITE ; LES GUITARES DU DIABLE - ROMEO : médium / R. STOLZ et J. BROUSSOLLE ; TRUMPET BOY et sa trompette-succès - ALMA DEL BANDONEON - tango / DISCEPOLO ; Antonio "Senor Tango" et son orchestre - LE RADAR A POGNON - rock / A. MESSIER ; Andrew WALRENCE et son orchestreBnF-Partenariats, Collection sonore - BelieveContient une table des matière

    Perdido cha cha ; Buenas Madres ; Mococo mambo ; Va mi négra / Nico Gomez et son orchestre typique

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    Comprend : Perdido cha-cha (2 min 30 s) - Buenas Madres (2 min 43 s) - Mococo mambo (2 min 35 s) - Va mi negra para la conga (2 min 10 s)BnF-Partenariats, Collection sonore - BelieveContient une table des matière

    Complete Mechanism for NO<sub><i>x</i></sub> Adsorption and Release on Atomically Dispersed Palladium in Pd-CHA

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    Palladium exchanged zeolites are an established class of passive NOx adsorber (PNA) that effectively stores and catalytically oxidizes NO at low temperature and releases NOx (x = 1, 2) at higher temperature, enabling its downstream selective catalytic reduction to N2. Notably, the presence of water leads to solvated and mobile Pd complexes that behave drastically different from the cationic species anchored to the zeolite framework under dry conditions. Herein, we use theoretical and experimental techniques to develop a complete reaction mechanism for NO adsorption and release on dynamically hydrated, isolated Pd ions within chabazite zeolites (Pd-CHA). van der Waals corrected density functional theory (DFT) calculations, subsequently verified with HSE06 single-point calculations, manifest that NO preferentially binds to the hydrated PdII site and facilitates the activation of water leading to the transient formation of a Brønsted acid site and hydrated palladium hydroxide. Adsorbed NO reacts with hydroxide to form HONO and Pd changes its formal oxidation state from +2 to +1 upon HONO desorption. HONO subsequently disproportionates to form NO, NO2, and H2O. The strong binding of NO to hydrated PdI leads to NO release at temperatures in excess of 200 °C with concomitant reoxidation by NO2 or O2. The complete NOx storage, oxidation, and release mechanisms identified by DFT are consistent with NOx uptake experiments on Pd-CHA. A consistent catalytic cycle for NO oxidation by water on isolated palladium active sites is described, and a mechanistic framework for further improvement of PNA performance is established

    Genung, Cha[rle]s H[arvey] an Herman Grimm (5 Briefe)

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    GENUNG, CHA[RLE]S H[ARVEY] AN HERMAN GRIMM (5 BRIEFE) Genung, Cha[rle]s H[arvey] an Herman Grimm (5 Briefe) (Br1123) Brief 1123 (Br1123) Brief 1124 (Br1124) Brief 1125 (Br1125) Brief 1126 (Br1126) Brief 1127 (Br1127

    Complete Mechanism for NO<sub><i>x</i></sub> Adsorption and Release on Atomically Dispersed Palladium in Pd-CHA

    No full text
    Palladium exchanged zeolites are an established class of passive NOx adsorber (PNA) that effectively stores and catalytically oxidizes NO at low temperature and releases NOx (x = 1, 2) at higher temperature, enabling its downstream selective catalytic reduction to N2. Notably, the presence of water leads to solvated and mobile Pd complexes that behave drastically different from the cationic species anchored to the zeolite framework under dry conditions. Herein, we use theoretical and experimental techniques to develop a complete reaction mechanism for NO adsorption and release on dynamically hydrated, isolated Pd ions within chabazite zeolites (Pd-CHA). van der Waals corrected density functional theory (DFT) calculations, subsequently verified with HSE06 single-point calculations, manifest that NO preferentially binds to the hydrated PdII site and facilitates the activation of water leading to the transient formation of a Brønsted acid site and hydrated palladium hydroxide. Adsorbed NO reacts with hydroxide to form HONO and Pd changes its formal oxidation state from +2 to +1 upon HONO desorption. HONO subsequently disproportionates to form NO, NO2, and H2O. The strong binding of NO to hydrated PdI leads to NO release at temperatures in excess of 200 °C with concomitant reoxidation by NO2 or O2. The complete NOx storage, oxidation, and release mechanisms identified by DFT are consistent with NOx uptake experiments on Pd-CHA. A consistent catalytic cycle for NO oxidation by water on isolated palladium active sites is described, and a mechanistic framework for further improvement of PNA performance is established
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